Isolation and characterization of simian T-cell leukemia virus type II from New World monkeys

Yi Ming A. Chen, Yng Ju Jang, Phyllis J. Kanki, Qian Chun Yu, Jaang Jiun Wang, Richard J. Montali, Kenneth P. Samuel, Takis S. Papas

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Since the description of human T-cell leukemia virus type I (HTLV-I) and its simian counterpart, simian T-cell leukemia virus type I (STLV-I), the possible existence of other related simian retroviruses has been raised. Here, we report a new retrovirus, STLV-II, which we have identified in spider monkeys (Ateles fusciceps), a New World primate species. Initially, a recombinant HTLV-II envelope protein (RP-IIB) was used to identify anti- STLV-II antibodies in New World monkeys by Western blot (immunoblot) assays. Subsequently, the virus was characterized by Southern blot hybridization, which showed that STLV-II and HTLV-II have a high degree of nucleotide sequence homology but have different restriction enzyme patterns. Nucleotide sequence analysis of the pX-II region of STLV-II provirus revealed 3% variation with the corresponding region of HTLV-II. Electron micrographic studies revealed HTLV-like, type C retrovirus particles outside the cell membranes of STLV-II-infected cells. This study describes the first link between HTLV-II and a simian reservoir in the New World. Further molecular studies of STLV-II infection in different species of New World monkeys, especially from the wild, may provide valuable information about the origin and intragroup relationships of South American monkeys. Spider monkeys infected with STLV-II may serve as an important animal model for HTLV-II infection in humans.

Original languageEnglish
Pages (from-to)1149-1157
Number of pages9
JournalJournal of Virology
Volume68
Issue number2
Publication statusPublished - Feb 1 1994
Externally publishedYes

Fingerprint

Retroviridae
Platyrrhini
Human T-lymphotropic virus 2
T-Cell Leukemia
Cebidae
Atelinae
leukemia
monkeys
T-lymphocytes
Viruses
viruses
Araneae
Primate T-lymphotropic virus 1
proviruses
nucleotide sequences
Ateles
HTLV-II Infections
Simian Retroviruses
Western Blotting
sequence homology

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Chen, Y. M. A., Jang, Y. J., Kanki, P. J., Yu, Q. C., Wang, J. J., Montali, R. J., ... Papas, T. S. (1994). Isolation and characterization of simian T-cell leukemia virus type II from New World monkeys. Journal of Virology, 68(2), 1149-1157.

Isolation and characterization of simian T-cell leukemia virus type II from New World monkeys. / Chen, Yi Ming A.; Jang, Yng Ju; Kanki, Phyllis J.; Yu, Qian Chun; Wang, Jaang Jiun; Montali, Richard J.; Samuel, Kenneth P.; Papas, Takis S.

In: Journal of Virology, Vol. 68, No. 2, 01.02.1994, p. 1149-1157.

Research output: Contribution to journalArticle

Chen, YMA, Jang, YJ, Kanki, PJ, Yu, QC, Wang, JJ, Montali, RJ, Samuel, KP & Papas, TS 1994, 'Isolation and characterization of simian T-cell leukemia virus type II from New World monkeys', Journal of Virology, vol. 68, no. 2, pp. 1149-1157.
Chen YMA, Jang YJ, Kanki PJ, Yu QC, Wang JJ, Montali RJ et al. Isolation and characterization of simian T-cell leukemia virus type II from New World monkeys. Journal of Virology. 1994 Feb 1;68(2):1149-1157.
Chen, Yi Ming A. ; Jang, Yng Ju ; Kanki, Phyllis J. ; Yu, Qian Chun ; Wang, Jaang Jiun ; Montali, Richard J. ; Samuel, Kenneth P. ; Papas, Takis S. / Isolation and characterization of simian T-cell leukemia virus type II from New World monkeys. In: Journal of Virology. 1994 ; Vol. 68, No. 2. pp. 1149-1157.
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