Isolation and Characterization of Human Muscle-derived Cells

Shing Hwa Lu, Chou Fu Wei, An Hang Yang, Michael B. Chancellor, Liang Shun Wang, Kuang Kuo Chen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objectives: To isolate and characterize human muscle-derived cells (MDCs) for future management applications on lower urinary tract symptoms, including stress urinary incontinence and bladder reconstitution. The development of muscle stem cells for transplantation or gene transfer in patients with muscle disorders has become more attractive and challenging recently. Methods: Human MDCs were isolated from the skeletal muscles of the limbs. The muscle tissues were minced, digested at 37°C by 0.2% collagenase, trypsinized, filtered, and cultured in F12 medium with 15% fetal bovine serum at 37°C. Human MDCs were then isolated using a modified preplate technique. After isolation, the MDCs were characterized by immunohistochemistry, flow cytometry, and indirect immunofluorescence. Results: The growth doubling time of the MDCs was approximately 24 hours. Immunohistochemistry study was performed with the stem cell markers CD34, CD117, vascular cell adhesion molecule, and vascular endothelial growth factor receptor 2, and the relative stem cell position was identified. Positive immunofluorescence outcomes were found with the stem cell markers, myoblast markers CXCR4, CD56, desmin, and a fibroblast marker AB-1. Flow cytometry analysis identified markers CD34 and CD56 in the isolated MDCs, with a percentage of 5.12% and 10.34%, respectively. Conclusions: The isolation and characterization of human MDCs was successfully achieved. Human MDCs might have the potential to be a novel tool for the management of stress urinary incontinence and bladder reconstitution.

Original languageEnglish
Pages (from-to)440-445
Number of pages6
JournalUrology
Volume74
Issue number2
DOIs
Publication statusPublished - Aug 2009
Externally publishedYes

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Muscle Cells
Stress Urinary Incontinence
Stem Cells
Flow Cytometry
Urinary Bladder
Immunohistochemistry
Patient Transfer
Vascular Endothelial Growth Factor Receptor-2
Lower Urinary Tract Symptoms
Desmin
Vascular Cell Adhesion Molecule-1
Myoblasts
Stem Cell Transplantation
Collagenases
Muscular Diseases
Indirect Fluorescent Antibody Technique
Fluorescent Antibody Technique
Skeletal Muscle
Extremities
Fibroblasts

ASJC Scopus subject areas

  • Urology

Cite this

Lu, S. H., Wei, C. F., Yang, A. H., Chancellor, M. B., Wang, L. S., & Chen, K. K. (2009). Isolation and Characterization of Human Muscle-derived Cells. Urology, 74(2), 440-445. https://doi.org/10.1016/j.urology.2009.01.048

Isolation and Characterization of Human Muscle-derived Cells. / Lu, Shing Hwa; Wei, Chou Fu; Yang, An Hang; Chancellor, Michael B.; Wang, Liang Shun; Chen, Kuang Kuo.

In: Urology, Vol. 74, No. 2, 08.2009, p. 440-445.

Research output: Contribution to journalArticle

Lu, SH, Wei, CF, Yang, AH, Chancellor, MB, Wang, LS & Chen, KK 2009, 'Isolation and Characterization of Human Muscle-derived Cells', Urology, vol. 74, no. 2, pp. 440-445. https://doi.org/10.1016/j.urology.2009.01.048
Lu SH, Wei CF, Yang AH, Chancellor MB, Wang LS, Chen KK. Isolation and Characterization of Human Muscle-derived Cells. Urology. 2009 Aug;74(2):440-445. https://doi.org/10.1016/j.urology.2009.01.048
Lu, Shing Hwa ; Wei, Chou Fu ; Yang, An Hang ; Chancellor, Michael B. ; Wang, Liang Shun ; Chen, Kuang Kuo. / Isolation and Characterization of Human Muscle-derived Cells. In: Urology. 2009 ; Vol. 74, No. 2. pp. 440-445.
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