Isoflurane attenuates dynorphin-induced cytotoxicity and downregulation of Bcl-2 expression in differentiated neuroblastoma SH-SY5Y cells

Gong-Jhe Wu, W. F. Chen, C. S. Sung, Y. H. Jean, C. H. Hung, F. A. Chen, M. H. Hsieh, Z. H. Wen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: It has been proposed that the volatile anesthetic isoflurane induces neuroprotection and that the endogenous opioid peptide dynorphin induces neurocytotoxicity in cells. The levels of dynorphin are often significantly elevated in neuropathophysiological conditions, and dynorphin can directly induce toxicity. However, the neuroprotective effects of isoflurane on dynorphin-induced cytotoxicity are still unclear. Methods: In order to determine the effect of isoflurane on dynorphin-induced cytotoxicity in neuronal cells, we have designed a device wherein cultured human neuroblastoma SH-SY5Y cells can be exposed to isoflurane. Fully differentiated SH-SY5Y cells were obtained by treating the cells with retinoic acid for 6 days. We examined SH-SY5Y cell survival, apoptosis, and antiapoptotic protein expression by cell viability, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling stain, and Western blot analysis, respectively. Results: After 16 h of dynorphin (10 μM) treatment, the SH-SY5Y cells showed significant cytotoxicity, apoptosis, and downregulation of the antiapoptotic Bcl-2 protein expression. These effects of dynorphin were significantly inhibited by isoflurane exposure for 32 h [pretreatment for 16 h and posttreatment (after dynorphin treatment) for 16 h]. Conclusion: Thus, our results suggest that isoflurane exerts neuroprotective effects in the case of dynorphin-induced pathophysiological disruption.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalActa Anaesthesiologica Scandinavica
Volume53
Issue number1
DOIs
Publication statusPublished - Jan 2009
Externally publishedYes

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Dynorphins
Isoflurane
Neuroblastoma
Down-Regulation
Neuroprotective Agents
Cell Survival
Apoptosis
Opioid Peptides
DNA Nucleotidylexotransferase
Tretinoin
Anesthetics
Proteins
Coloring Agents
Western Blotting
Equipment and Supplies

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Isoflurane attenuates dynorphin-induced cytotoxicity and downregulation of Bcl-2 expression in differentiated neuroblastoma SH-SY5Y cells. / Wu, Gong-Jhe; Chen, W. F.; Sung, C. S.; Jean, Y. H.; Hung, C. H.; Chen, F. A.; Hsieh, M. H.; Wen, Z. H.

In: Acta Anaesthesiologica Scandinavica, Vol. 53, No. 1, 01.2009, p. 55-60.

Research output: Contribution to journalArticle

Wu, Gong-Jhe ; Chen, W. F. ; Sung, C. S. ; Jean, Y. H. ; Hung, C. H. ; Chen, F. A. ; Hsieh, M. H. ; Wen, Z. H. / Isoflurane attenuates dynorphin-induced cytotoxicity and downregulation of Bcl-2 expression in differentiated neuroblastoma SH-SY5Y cells. In: Acta Anaesthesiologica Scandinavica. 2009 ; Vol. 53, No. 1. pp. 55-60.
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