Isocupressic acid blocks progesterone production from bovine luteal cells

L. S. Wu, Joyce C. Chen, S. Y. Sheu, C. C. Huang, Y. H. Kuo, C. H. Chiu, W. X. Lian, C. J. Yang, K. Kaphle, Jen Hsou Lin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The needles of ponderosa pine (Pinus ponderosa Laws.) were reported to induce abortions when fed to late-term pregnant beef cows in North America. An in vivo study of pregnant cows suggested that isocupressic acid (IA) was the main abortifacient isolated from needles and bark of the pine. However, the mechanism of abortifacient activity of IA is not clear yet. In a pregnant cow, the corpus luteum of the ovary helps the maintenance of pregnancy by its progesterone production. This study involved the IA extracted from the root of the Taiwan cypress (Juniperus formosana) and used a frozen-thawed bovine luteal cell culture system to investigate the action of IA on progesterone production. Thawed bovine luteal cells (1 × 105 cells/ml/well) in M199 medium were cultured in 24-well culture plates at 37°C in a 5% CO 2 incubator. Ten ml of tested drugs, IA at 1 to 1000 ng/ ml and/or ovine luteinizing hormone (oLH) at 1 to 100 ng/μ1 or 8-bromo-cyclic adenosine monophosphate (8-Br-cAMP) with 0.1-10 mM, were added into each well. After 4 hours of incubation, the media were harvested and assayed for progesterone by an enzyme immunoassay. Progesterone production from cells was the indicator used to evaluate the action of IA. All tested doses of IA significantly inhibited progesterone production in both basal and oLH stimulating conditions. Also those dosages inhibited cyclic adenosine-3',5'- monophosphate (cAMP) stimulation, suggesting a post-cAMP mechanism is involved in the IA action. We concluded that IA can induce pregnant cows to abort partly through blocking luteal function and may be identified as a new abortifacient chemical.

Original languageEnglish
Pages (from-to)533-541
Number of pages9
JournalAmerican Journal of Chinese Medicine
Volume30
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

Luteal Cells
Progesterone
Abortifacient Agents
Pinus ponderosa
Corpus Luteum
Luteinizing Hormone
Cyclic AMP
Needles
Sheep
Cupressus
Juniperus
Pregnancy Maintenance
8-Bromo Cyclic Adenosine Monophosphate
isocupressic acid
Incubators
Carbon Monoxide
North America
Immunoenzyme Techniques
Taiwan
Ovary

Keywords

  • Bovine Luteal Cells
  • Enzyme Immunoassay
  • Isocupressic Acid
  • Ponderosa Pine
  • Progesterone

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Isocupressic acid blocks progesterone production from bovine luteal cells. / Wu, L. S.; Chen, Joyce C.; Sheu, S. Y.; Huang, C. C.; Kuo, Y. H.; Chiu, C. H.; Lian, W. X.; Yang, C. J.; Kaphle, K.; Lin, Jen Hsou.

In: American Journal of Chinese Medicine, Vol. 30, No. 4, 2002, p. 533-541.

Research output: Contribution to journalArticle

Wu, LS, Chen, JC, Sheu, SY, Huang, CC, Kuo, YH, Chiu, CH, Lian, WX, Yang, CJ, Kaphle, K & Lin, JH 2002, 'Isocupressic acid blocks progesterone production from bovine luteal cells', American Journal of Chinese Medicine, vol. 30, no. 4, pp. 533-541. https://doi.org/10.1142/S0192415X02000508
Wu, L. S. ; Chen, Joyce C. ; Sheu, S. Y. ; Huang, C. C. ; Kuo, Y. H. ; Chiu, C. H. ; Lian, W. X. ; Yang, C. J. ; Kaphle, K. ; Lin, Jen Hsou. / Isocupressic acid blocks progesterone production from bovine luteal cells. In: American Journal of Chinese Medicine. 2002 ; Vol. 30, No. 4. pp. 533-541.
@article{dca954964d8941eeb16f5e78a2883838,
title = "Isocupressic acid blocks progesterone production from bovine luteal cells",
abstract = "The needles of ponderosa pine (Pinus ponderosa Laws.) were reported to induce abortions when fed to late-term pregnant beef cows in North America. An in vivo study of pregnant cows suggested that isocupressic acid (IA) was the main abortifacient isolated from needles and bark of the pine. However, the mechanism of abortifacient activity of IA is not clear yet. In a pregnant cow, the corpus luteum of the ovary helps the maintenance of pregnancy by its progesterone production. This study involved the IA extracted from the root of the Taiwan cypress (Juniperus formosana) and used a frozen-thawed bovine luteal cell culture system to investigate the action of IA on progesterone production. Thawed bovine luteal cells (1 × 105 cells/ml/well) in M199 medium were cultured in 24-well culture plates at 37°C in a 5{\%} CO 2 incubator. Ten ml of tested drugs, IA at 1 to 1000 ng/ ml and/or ovine luteinizing hormone (oLH) at 1 to 100 ng/μ1 or 8-bromo-cyclic adenosine monophosphate (8-Br-cAMP) with 0.1-10 mM, were added into each well. After 4 hours of incubation, the media were harvested and assayed for progesterone by an enzyme immunoassay. Progesterone production from cells was the indicator used to evaluate the action of IA. All tested doses of IA significantly inhibited progesterone production in both basal and oLH stimulating conditions. Also those dosages inhibited cyclic adenosine-3',5'- monophosphate (cAMP) stimulation, suggesting a post-cAMP mechanism is involved in the IA action. We concluded that IA can induce pregnant cows to abort partly through blocking luteal function and may be identified as a new abortifacient chemical.",
keywords = "Bovine Luteal Cells, Enzyme Immunoassay, Isocupressic Acid, Ponderosa Pine, Progesterone",
author = "Wu, {L. S.} and Chen, {Joyce C.} and Sheu, {S. Y.} and Huang, {C. C.} and Kuo, {Y. H.} and Chiu, {C. H.} and Lian, {W. X.} and Yang, {C. J.} and K. Kaphle and Lin, {Jen Hsou}",
year = "2002",
doi = "10.1142/S0192415X02000508",
language = "English",
volume = "30",
pages = "533--541",
journal = "American Journal of Chinese Medicine",
issn = "0192-415X",
publisher = "World Scientific Publishing Co. Pte Ltd",
number = "4",

}

TY - JOUR

T1 - Isocupressic acid blocks progesterone production from bovine luteal cells

AU - Wu, L. S.

AU - Chen, Joyce C.

AU - Sheu, S. Y.

AU - Huang, C. C.

AU - Kuo, Y. H.

AU - Chiu, C. H.

AU - Lian, W. X.

AU - Yang, C. J.

AU - Kaphle, K.

AU - Lin, Jen Hsou

PY - 2002

Y1 - 2002

N2 - The needles of ponderosa pine (Pinus ponderosa Laws.) were reported to induce abortions when fed to late-term pregnant beef cows in North America. An in vivo study of pregnant cows suggested that isocupressic acid (IA) was the main abortifacient isolated from needles and bark of the pine. However, the mechanism of abortifacient activity of IA is not clear yet. In a pregnant cow, the corpus luteum of the ovary helps the maintenance of pregnancy by its progesterone production. This study involved the IA extracted from the root of the Taiwan cypress (Juniperus formosana) and used a frozen-thawed bovine luteal cell culture system to investigate the action of IA on progesterone production. Thawed bovine luteal cells (1 × 105 cells/ml/well) in M199 medium were cultured in 24-well culture plates at 37°C in a 5% CO 2 incubator. Ten ml of tested drugs, IA at 1 to 1000 ng/ ml and/or ovine luteinizing hormone (oLH) at 1 to 100 ng/μ1 or 8-bromo-cyclic adenosine monophosphate (8-Br-cAMP) with 0.1-10 mM, were added into each well. After 4 hours of incubation, the media were harvested and assayed for progesterone by an enzyme immunoassay. Progesterone production from cells was the indicator used to evaluate the action of IA. All tested doses of IA significantly inhibited progesterone production in both basal and oLH stimulating conditions. Also those dosages inhibited cyclic adenosine-3',5'- monophosphate (cAMP) stimulation, suggesting a post-cAMP mechanism is involved in the IA action. We concluded that IA can induce pregnant cows to abort partly through blocking luteal function and may be identified as a new abortifacient chemical.

AB - The needles of ponderosa pine (Pinus ponderosa Laws.) were reported to induce abortions when fed to late-term pregnant beef cows in North America. An in vivo study of pregnant cows suggested that isocupressic acid (IA) was the main abortifacient isolated from needles and bark of the pine. However, the mechanism of abortifacient activity of IA is not clear yet. In a pregnant cow, the corpus luteum of the ovary helps the maintenance of pregnancy by its progesterone production. This study involved the IA extracted from the root of the Taiwan cypress (Juniperus formosana) and used a frozen-thawed bovine luteal cell culture system to investigate the action of IA on progesterone production. Thawed bovine luteal cells (1 × 105 cells/ml/well) in M199 medium were cultured in 24-well culture plates at 37°C in a 5% CO 2 incubator. Ten ml of tested drugs, IA at 1 to 1000 ng/ ml and/or ovine luteinizing hormone (oLH) at 1 to 100 ng/μ1 or 8-bromo-cyclic adenosine monophosphate (8-Br-cAMP) with 0.1-10 mM, were added into each well. After 4 hours of incubation, the media were harvested and assayed for progesterone by an enzyme immunoassay. Progesterone production from cells was the indicator used to evaluate the action of IA. All tested doses of IA significantly inhibited progesterone production in both basal and oLH stimulating conditions. Also those dosages inhibited cyclic adenosine-3',5'- monophosphate (cAMP) stimulation, suggesting a post-cAMP mechanism is involved in the IA action. We concluded that IA can induce pregnant cows to abort partly through blocking luteal function and may be identified as a new abortifacient chemical.

KW - Bovine Luteal Cells

KW - Enzyme Immunoassay

KW - Isocupressic Acid

KW - Ponderosa Pine

KW - Progesterone

UR - http://www.scopus.com/inward/record.url?scp=0037676216&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037676216&partnerID=8YFLogxK

U2 - 10.1142/S0192415X02000508

DO - 10.1142/S0192415X02000508

M3 - Article

C2 - 12568280

AN - SCOPUS:0037676216

VL - 30

SP - 533

EP - 541

JO - American Journal of Chinese Medicine

JF - American Journal of Chinese Medicine

SN - 0192-415X

IS - 4

ER -