Ischemic preconditioning in hepatic ischemic–reperfusion injury

Sheng Chih Kuo, Yueh Wei Liu, Ching Hua Tsai, Shyr Ming Sheen-Chen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background/Introduction Ischemic preconditioning is a method in which brief periods of ischemia render tissues resistant to injury resulting from prolonged ischemia and reperfusion, so-called ischemia–reperfusion injury. Purpose To elucidate the possible protective role of ischemic preconditioning in rat livers with ischemia–reperfusion injury. Methods Rats were first allocated to either a sham control or an ischemic preconditioning group. On the following day, the rats from each group were administered either 30 minutes or 45 minutes hepatic ischemia. Next, rat livers were harvested for measuring proliferating cell nuclear antigen, heme oxygenase-1, inducible nitric oxide synthase, and heat shock protein 70 mRNA levels. Results Both proliferating cell nuclear antigen and hemeoxygenase-1 expression increased significantly after 45 minutes hepatic ischemia compared with those after 30 minutes hepatic ischemia, but they decreased significantly with ischemic preconditioning. However, ischemic preconditioning did not affect inducible nitric oxide synthase or heat shock protein 70 expression. Conclusion From the preliminary findings, further elucidation is warranted.

Original languageEnglish
Pages (from-to)169-173
Number of pages5
JournalFormosan Journal of Surgery
Volume49
Issue number5
DOIs
Publication statusPublished - Oct 1 2016
Externally publishedYes

Fingerprint

Ischemic Preconditioning
Ischemia
Liver
Wounds and Injuries
Heme Oxygenase-1
HSP70 Heat-Shock Proteins
Proliferating Cell Nuclear Antigen
Nitric Oxide Synthase Type II
Reperfusion
Messenger RNA

Keywords

  • ischemia–reperfusion injury
  • liver
  • preconditioning

ASJC Scopus subject areas

  • Surgery

Cite this

Ischemic preconditioning in hepatic ischemic–reperfusion injury. / Kuo, Sheng Chih; Liu, Yueh Wei; Tsai, Ching Hua; Sheen-Chen, Shyr Ming.

In: Formosan Journal of Surgery, Vol. 49, No. 5, 01.10.2016, p. 169-173.

Research output: Contribution to journalArticle

Kuo, Sheng Chih ; Liu, Yueh Wei ; Tsai, Ching Hua ; Sheen-Chen, Shyr Ming. / Ischemic preconditioning in hepatic ischemic–reperfusion injury. In: Formosan Journal of Surgery. 2016 ; Vol. 49, No. 5. pp. 169-173.
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