Is frying oil a dietary source of an endocrine disruptor? Anti-estrogenic effects of polar compounds from frying oil in rats

Yu Shun Lin, Shui Yuan Lu, Hai Ping Wu, Chi Fen Chang, Yung Tsung Chiu, Hui Ting Yang, Pei Min Chao

Research output: Contribution to journalArticle

Abstract

The objective was to investigate endocrine-disrupting effects of polar compounds from oxidized frying oil. Estrogenicity of polar compounds was tested with a rat uterotrophic bioassay. Dietary oxidized frying oil (containing 51% polar compounds) or polar compounds isolated from it were incorporated into feed (in lieu of fresh soybean oil) and fed to ovariectomized rats, with or without treatment with exogenous ethynyl estradiol. Exogenous estrogen restored uterine weight, and caused histological abnormalities (stratified epithelia and conglomerate glands) as well as proliferation of uterine epithelial cells. However, tamoxifen or polar compounds reduced these effects. Furthermore, tamoxifen or polar compounds down-regulated uterine mRNA expression of estrogen receptor (ER)-target genes, implicating reduced ER activity in this hypo-uterotrophic effect. Inhibition of ER signaling and mitosis by polar compounds were attributed to reduced MAPK and AKT activation, as well as a reduced ligand binding domain-transactivity of ERα/β. We concluded polar compounds from frying oil are potential endocrine-disrupting chemicals, with implications for food and environmental safety.

Original languageEnglish
Pages (from-to)18-27
Number of pages10
JournalEcotoxicology and Environmental Safety
Volume169
DOIs
Publication statusPublished - Mar 1 2019
Externally publishedYes

Fingerprint

Unsaturated Dietary Fats
Endocrine Disruptors
Estrogen Receptors
Rats
Estrogens
Oils
Tamoxifen
Soybean Oil
Ethinyl Estradiol
Food Safety
Soybean oil
Mitosis
Biological Assay
Bioassay
Epithelium
Epithelial Cells
Ligands
Weights and Measures
Messenger RNA
Genes

Keywords

  • Anti-estrogenic effect
  • Oxidized frying oil
  • Polar compounds
  • Uterotrophic bioassay

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Is frying oil a dietary source of an endocrine disruptor? Anti-estrogenic effects of polar compounds from frying oil in rats. / Lin, Yu Shun; Lu, Shui Yuan; Wu, Hai Ping; Chang, Chi Fen; Chiu, Yung Tsung; Yang, Hui Ting; Chao, Pei Min.

In: Ecotoxicology and Environmental Safety, Vol. 169, 01.03.2019, p. 18-27.

Research output: Contribution to journalArticle

Lin, Yu Shun ; Lu, Shui Yuan ; Wu, Hai Ping ; Chang, Chi Fen ; Chiu, Yung Tsung ; Yang, Hui Ting ; Chao, Pei Min. / Is frying oil a dietary source of an endocrine disruptor? Anti-estrogenic effects of polar compounds from frying oil in rats. In: Ecotoxicology and Environmental Safety. 2019 ; Vol. 169. pp. 18-27.
@article{0d67966edb8c46148b6b0b64e31ab9e3,
title = "Is frying oil a dietary source of an endocrine disruptor? Anti-estrogenic effects of polar compounds from frying oil in rats",
abstract = "The objective was to investigate endocrine-disrupting effects of polar compounds from oxidized frying oil. Estrogenicity of polar compounds was tested with a rat uterotrophic bioassay. Dietary oxidized frying oil (containing 51{\%} polar compounds) or polar compounds isolated from it were incorporated into feed (in lieu of fresh soybean oil) and fed to ovariectomized rats, with or without treatment with exogenous ethynyl estradiol. Exogenous estrogen restored uterine weight, and caused histological abnormalities (stratified epithelia and conglomerate glands) as well as proliferation of uterine epithelial cells. However, tamoxifen or polar compounds reduced these effects. Furthermore, tamoxifen or polar compounds down-regulated uterine mRNA expression of estrogen receptor (ER)-target genes, implicating reduced ER activity in this hypo-uterotrophic effect. Inhibition of ER signaling and mitosis by polar compounds were attributed to reduced MAPK and AKT activation, as well as a reduced ligand binding domain-transactivity of ERα/β. We concluded polar compounds from frying oil are potential endocrine-disrupting chemicals, with implications for food and environmental safety.",
keywords = "Anti-estrogenic effect, Oxidized frying oil, Polar compounds, Uterotrophic bioassay",
author = "Lin, {Yu Shun} and Lu, {Shui Yuan} and Wu, {Hai Ping} and Chang, {Chi Fen} and Chiu, {Yung Tsung} and Yang, {Hui Ting} and Chao, {Pei Min}",
year = "2019",
month = "3",
day = "1",
doi = "10.1016/j.ecoenv.2018.10.111",
language = "English",
volume = "169",
pages = "18--27",
journal = "Ecotoxicology and Environmental Safety",
issn = "0147-6513",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Is frying oil a dietary source of an endocrine disruptor? Anti-estrogenic effects of polar compounds from frying oil in rats

AU - Lin, Yu Shun

AU - Lu, Shui Yuan

AU - Wu, Hai Ping

AU - Chang, Chi Fen

AU - Chiu, Yung Tsung

AU - Yang, Hui Ting

AU - Chao, Pei Min

PY - 2019/3/1

Y1 - 2019/3/1

N2 - The objective was to investigate endocrine-disrupting effects of polar compounds from oxidized frying oil. Estrogenicity of polar compounds was tested with a rat uterotrophic bioassay. Dietary oxidized frying oil (containing 51% polar compounds) or polar compounds isolated from it were incorporated into feed (in lieu of fresh soybean oil) and fed to ovariectomized rats, with or without treatment with exogenous ethynyl estradiol. Exogenous estrogen restored uterine weight, and caused histological abnormalities (stratified epithelia and conglomerate glands) as well as proliferation of uterine epithelial cells. However, tamoxifen or polar compounds reduced these effects. Furthermore, tamoxifen or polar compounds down-regulated uterine mRNA expression of estrogen receptor (ER)-target genes, implicating reduced ER activity in this hypo-uterotrophic effect. Inhibition of ER signaling and mitosis by polar compounds were attributed to reduced MAPK and AKT activation, as well as a reduced ligand binding domain-transactivity of ERα/β. We concluded polar compounds from frying oil are potential endocrine-disrupting chemicals, with implications for food and environmental safety.

AB - The objective was to investigate endocrine-disrupting effects of polar compounds from oxidized frying oil. Estrogenicity of polar compounds was tested with a rat uterotrophic bioassay. Dietary oxidized frying oil (containing 51% polar compounds) or polar compounds isolated from it were incorporated into feed (in lieu of fresh soybean oil) and fed to ovariectomized rats, with or without treatment with exogenous ethynyl estradiol. Exogenous estrogen restored uterine weight, and caused histological abnormalities (stratified epithelia and conglomerate glands) as well as proliferation of uterine epithelial cells. However, tamoxifen or polar compounds reduced these effects. Furthermore, tamoxifen or polar compounds down-regulated uterine mRNA expression of estrogen receptor (ER)-target genes, implicating reduced ER activity in this hypo-uterotrophic effect. Inhibition of ER signaling and mitosis by polar compounds were attributed to reduced MAPK and AKT activation, as well as a reduced ligand binding domain-transactivity of ERα/β. We concluded polar compounds from frying oil are potential endocrine-disrupting chemicals, with implications for food and environmental safety.

KW - Anti-estrogenic effect

KW - Oxidized frying oil

KW - Polar compounds

KW - Uterotrophic bioassay

UR - http://www.scopus.com/inward/record.url?scp=85056199098&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056199098&partnerID=8YFLogxK

U2 - 10.1016/j.ecoenv.2018.10.111

DO - 10.1016/j.ecoenv.2018.10.111

M3 - Article

VL - 169

SP - 18

EP - 27

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

SN - 0147-6513

ER -