Abstract

Introduction: Recent studies have revealed that nanoscale structure (nanotopography) of extracellular matrix is an important regulator of cell biology. However, it remains unknown whether nanotopography regulates cancer cell behaviors upon exposure to ionizing radiation (IR). Materials and Methods: Hepatoma cell lines and a squamous cell carcinoma cell line were cultured on nanopatterned polycaprolactone surfaces and were irradiated. Sphere formation was counted and expression of cancer stemness-related genes (Sox2, Nanog and Oct3/4) was measured with quantitative polymerase chain reaction. Results: In this study, we showed that IR induced expression of stemness genes including Sox2, Nanog and Oct3/4 in several cancer cell lines. Growing J5 hepatoma cells on a square lattice of evenly-spaced nanopits made of polycaprolactone attenuates cancer stemness genes expression induced by IR. Growing J5 cells on a near-square lattice of nanopits has smaller effects on IR-induced cancer stemness genes expression. The phenomenon was reproducible in another cell line derived from squamous cell carcinoma. Conclusions: We provided experimental evidence that radiation-induced expression of cancer stemness genes in cancer cells is regulated by nanotopography of the attaching surface. Further research is warranted to analyze molecular interaction between integrin signaling and cancer stemness, as well as the implications for cancer radiotherapy.
Original languageEnglish
Pages (from-to)13-20
Number of pages8
Journal放射治療與腫瘤學
Volume25
Issue number1
DOIs
Publication statusPublished - 2018

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Radiation-Induced Neoplasms
Neoplasm Genes
Ionizing Radiation
Cell Line
Neoplasms
Gene Expression
Hepatocellular Carcinoma
Squamous Cell Carcinoma
Integrins
Extracellular Matrix
Cell Biology
Radiotherapy
Polymerase Chain Reaction
Research
Genes

Keywords

  • Nanotopography
  • Extracellular matrix
  • Cancer stemness
  • Ionizing radiation
  • 奈米表面結構
  • 細胞外間質
  • 癌幹細胞特性
  • 游離輻射

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IONIZING RADIATION-INDUCED CANCER STEMNESS GENE EXPRESSI ON IS REGULATED BY MATRI X NANOTOPOGRAPHY. / Lu, Long-Sheng; Chen, Yin-Ju ; Lin, Yun-Tien ; Lee, Jing-Ting ; Chiou, Jeng-Fong .

In: 放射治療與腫瘤學, Vol. 25, No. 1, 2018, p. 13-20.

Research output: Contribution to journalArticle

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title = "IONIZING RADIATION-INDUCED CANCER STEMNESS GENE EXPRESSI ON IS REGULATED BY MATRI X NANOTOPOGRAPHY",
abstract = "Introduction: Recent studies have revealed that nanoscale structure (nanotopography) of extracellular matrix is an important regulator of cell biology. However, it remains unknown whether nanotopography regulates cancer cell behaviors upon exposure to ionizing radiation (IR). Materials and Methods: Hepatoma cell lines and a squamous cell carcinoma cell line were cultured on nanopatterned polycaprolactone surfaces and were irradiated. Sphere formation was counted and expression of cancer stemness-related genes (Sox2, Nanog and Oct3/4) was measured with quantitative polymerase chain reaction. Results: In this study, we showed that IR induced expression of stemness genes including Sox2, Nanog and Oct3/4 in several cancer cell lines. Growing J5 hepatoma cells on a square lattice of evenly-spaced nanopits made of polycaprolactone attenuates cancer stemness genes expression induced by IR. Growing J5 cells on a near-square lattice of nanopits has smaller effects on IR-induced cancer stemness genes expression. The phenomenon was reproducible in another cell line derived from squamous cell carcinoma. Conclusions: We provided experimental evidence that radiation-induced expression of cancer stemness genes in cancer cells is regulated by nanotopography of the attaching surface. Further research is warranted to analyze molecular interaction between integrin signaling and cancer stemness, as well as the implications for cancer radiotherapy.",
keywords = "Nanotopography, Extracellular matrix, Cancer stemness, Ionizing radiation, 奈米表面結構, 細胞外間質, 癌幹細胞特性, 游離輻射, Nanotopography, Extracellular matrix, Cancer stemness, Ionizing radiation",
author = "Long-Sheng Lu and Yin-Ju Chen and Yun-Tien Lin and Jing-Ting Lee and Jeng-Fong Chiou",
year = "2018",
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language = "English",
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pages = "13--20",
journal = "放射治療與腫瘤學",
issn = "1023-988x",
publisher = "台灣放射腫瘤學會",
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T1 - IONIZING RADIATION-INDUCED CANCER STEMNESS GENE EXPRESSI ON IS REGULATED BY MATRI X NANOTOPOGRAPHY

AU - Lu, Long-Sheng

AU - Chen, Yin-Ju

AU - Lin, Yun-Tien

AU - Lee, Jing-Ting

AU - Chiou, Jeng-Fong

PY - 2018

Y1 - 2018

N2 - Introduction: Recent studies have revealed that nanoscale structure (nanotopography) of extracellular matrix is an important regulator of cell biology. However, it remains unknown whether nanotopography regulates cancer cell behaviors upon exposure to ionizing radiation (IR). Materials and Methods: Hepatoma cell lines and a squamous cell carcinoma cell line were cultured on nanopatterned polycaprolactone surfaces and were irradiated. Sphere formation was counted and expression of cancer stemness-related genes (Sox2, Nanog and Oct3/4) was measured with quantitative polymerase chain reaction. Results: In this study, we showed that IR induced expression of stemness genes including Sox2, Nanog and Oct3/4 in several cancer cell lines. Growing J5 hepatoma cells on a square lattice of evenly-spaced nanopits made of polycaprolactone attenuates cancer stemness genes expression induced by IR. Growing J5 cells on a near-square lattice of nanopits has smaller effects on IR-induced cancer stemness genes expression. The phenomenon was reproducible in another cell line derived from squamous cell carcinoma. Conclusions: We provided experimental evidence that radiation-induced expression of cancer stemness genes in cancer cells is regulated by nanotopography of the attaching surface. Further research is warranted to analyze molecular interaction between integrin signaling and cancer stemness, as well as the implications for cancer radiotherapy.

AB - Introduction: Recent studies have revealed that nanoscale structure (nanotopography) of extracellular matrix is an important regulator of cell biology. However, it remains unknown whether nanotopography regulates cancer cell behaviors upon exposure to ionizing radiation (IR). Materials and Methods: Hepatoma cell lines and a squamous cell carcinoma cell line were cultured on nanopatterned polycaprolactone surfaces and were irradiated. Sphere formation was counted and expression of cancer stemness-related genes (Sox2, Nanog and Oct3/4) was measured with quantitative polymerase chain reaction. Results: In this study, we showed that IR induced expression of stemness genes including Sox2, Nanog and Oct3/4 in several cancer cell lines. Growing J5 hepatoma cells on a square lattice of evenly-spaced nanopits made of polycaprolactone attenuates cancer stemness genes expression induced by IR. Growing J5 cells on a near-square lattice of nanopits has smaller effects on IR-induced cancer stemness genes expression. The phenomenon was reproducible in another cell line derived from squamous cell carcinoma. Conclusions: We provided experimental evidence that radiation-induced expression of cancer stemness genes in cancer cells is regulated by nanotopography of the attaching surface. Further research is warranted to analyze molecular interaction between integrin signaling and cancer stemness, as well as the implications for cancer radiotherapy.

KW - Nanotopography

KW - Extracellular matrix

KW - Cancer stemness

KW - Ionizing radiation

KW - 奈米表面結構

KW - 細胞外間質

KW - 癌幹細胞特性

KW - 游離輻射

KW - Nanotopography

KW - Extracellular matrix

KW - Cancer stemness

KW - Ionizing radiation

U2 - 10.6316/TRO/201825

DO - 10.6316/TRO/201825

M3 - Article

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JF - 放射治療與腫瘤學

SN - 1023-988x

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ER -