Ion-pair assisted recovery of matrix-assisted laser desorption/ionization mass spectral signals from SDS-containing peptide-protein mixtures

Rajendram V. Rajnarayanan, Kuan Wang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have developed a simple and effective means of using alkylammonium ion-pairing agents, such as cetyltetramethylammonium bromide, to recover matrix assisted laser desorption/ionization mass spectrometric (MALDI-MS) signals from sodium dodecyl sulfate (SDS)-containing protein and peptide samples. A two-layer method of matrix preparation, with a bottom matrix layer of ion-pairing agents and a top matrix layer of SDS protein samples, is essential for reproducible MALDI mass spectra with good recovery. Both buffer ions and ion-pairing agents have profound effects on signal recovery and can be rapidly and systematically optimized. This practical technique, termed ion-pair assisted recovery (IPAR), is compatible with major SDS-based biotechniques and can be easily incorporated into high-throughput proteomic analysis.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalJournal of Mass Spectrometry
Volume39
Issue number1
DOIs
Publication statusPublished - Jan 2004
Externally publishedYes

Keywords

  • Ion pair
  • Mass spectrometry
  • Matrix-assisted laser desorption/ionization
  • Peptide
  • Protein
  • Sodium dodecyl sulfate

ASJC Scopus subject areas

  • Spectroscopy

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