Abstract
We have developed a simple and effective means of using alkylammonium ion-pairing agents, such as cetyltetramethylammonium bromide, to recover matrix assisted laser desorption/ionization mass spectrometric (MALDI-MS) signals from sodium dodecyl sulfate (SDS)-containing protein and peptide samples. A two-layer method of matrix preparation, with a bottom matrix layer of ion-pairing agents and a top matrix layer of SDS protein samples, is essential for reproducible MALDI mass spectra with good recovery. Both buffer ions and ion-pairing agents have profound effects on signal recovery and can be rapidly and systematically optimized. This practical technique, termed ion-pair assisted recovery (IPAR), is compatible with major SDS-based biotechniques and can be easily incorporated into high-throughput proteomic analysis.
Original language | English |
---|---|
Pages (from-to) | 79-85 |
Number of pages | 7 |
Journal | Journal of Mass Spectrometry |
Volume | 39 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2004 |
Externally published | Yes |
Keywords
- Ion pair
- Mass spectrometry
- Matrix-assisted laser desorption/ionization
- Peptide
- Protein
- Sodium dodecyl sulfate
ASJC Scopus subject areas
- Spectroscopy