Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages

Jam Inn Tzeng, Bing Chang Chen, Huey Mei Chang, Jhi Joung Wang, Munisamy Sureshbabu, Ming Hsien Chien, Ming Jen Hsu, Mauo Ying Bien, Wen Ta Chiu, Chuang Ye Hong, Chien Huang Lin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-κB (NF-κB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in κB-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-κB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages.

Original languageEnglish
Pages (from-to)162-166
Number of pages5
JournalPharmacological Research
Volume61
Issue number2
DOIs
Publication statusPublished - Feb 2010

Keywords

  • COX-2
  • Inflammation
  • NF-κB
  • Peptidoglycan
  • Protein kinase C

ASJC Scopus subject areas

  • Pharmacology

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