Abstract

We have investigated the role of protein kinase C (PKC) and nuclear factor-κB (NF-κB) in cyclooxygenase-2 (COX-2) expression caused by Staphylococcus aureus lipoteichoic acid in RAW 264.7 macrophages. A phosphatidylcholine-phospholipase C (PC-PLC) inhibitor (D-609) and a phosphatidyl-inositol-phospholipase C (PI-PLC) inhibitor (U-73122) attenuated lipoteichoic acid-induced COX-2 expression, while a phosphatidate phosphohydrolase inhibitor (propranolol) had no effect. Two PKC inhibitors (Go 6976 and Ro 31-8220) and the NF-κB inhibitor, pyrrolidine dithiocarbamate (PDTC), also attenuated lipoteichoic acid-induced COX-2 expression. Lipoteichoic acid resulted in a decrease in PKC activity in the cytosol and an increase in PKC activity in membranes. The lipoteichoic acid-induced translocation of p65 NF-κB from the cytosol to the nucleus was inhibited by D-609, U-73122, Go 6976, Ro 31-8220, and PDTC, but not by propranolol. The results suggested that lipoteichoic acid might have activated PC-PLC and PI-PLC to induce PKC activation, which in turn initiated NF-κB activation, and finally induced COX-2 expression in RAW 264.7 macrophages.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
JournalJournal of Pharmacy and Pharmacology
Volume55
Issue number1
Publication statusPublished - Jan 1 2003

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Cyclooxygenase 2
Protein Kinase C
Macrophages
Type C Phospholipases
Phosphatidylinositols
Phosphatidylcholines
Propranolol
Cytosol
Phosphatidate Phosphatase
Protein C Inhibitor
Protein Kinase Inhibitors
Staphylococcus aureus
lipoteichoic acid
Membranes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

Involvement of nuclear factor-κB in lipoteichoic acid-induced cyclooxygenase-2 expression in RAW 264.7 macrophages. / Chiang, L. L.; Kuo, C. T.; Wang, C. H.; Chen, Tzeng-Fu; Ho, Y. S.; Kuo, H. P.; Lin, C. H.

In: Journal of Pharmacy and Pharmacology, Vol. 55, No. 1, 01.01.2003, p. 115-123.

Research output: Contribution to journalArticle

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AU - Chen, Tzeng-Fu

AU - Ho, Y. S.

AU - Kuo, H. P.

AU - Lin, C. H.

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