Abstract

The metal nickel (Ni2+) is found everywhere in our daily lives, including coins, costume jewelry, and even nuts and chocolates. Nickel poisoning can cause inflammatory reactions, respiratory diseases, and allergic contact dermatitis. To clarify the mechanism by which nickel induces mediators of inflammation, we used the human acute monocytic leukemia THP-1 cell line as a model. Interleukin (IL)-8 promoter activity as well as gene expression were tested by luciferase assay and real-time polymerase chain reaction. The underlying mechanisms of nickel-induced IL-8 were investigated. We found that nickel induced IL-8 gene expression via the L-type Ca2+ channel, Toll-like receptor-4 (TRL-4) and nuclear factor NF-κB signal transduction pathways. Nickel activated NF-κB expression through extracellular signal-regulated kinase 1/2 phosphorylation and then increased IL-8 expression. Thus, the L-type Ca2+ channel and TRL-4 play important roles in nickel-induced inflammatory gene expressions.

Original languageEnglish
Pages (from-to)5-12
Number of pages8
JournalEnvironmental Toxicology
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Toll-Like Receptor 4
Nickel
Interleukin-8
Gene expression
gene expression
nickel
Gene Expression
Jewelry
Dermatitis
Leukemia, Monocytic, Acute
Inflammation Mediators
Signal transduction
Pulmonary diseases
Numismatics
Phosphorylation
Allergic Contact Dermatitis
Nuts
respiratory disease
Mitogen-Activated Protein Kinase 3
nut

Keywords

  • Human acute monocytic leukemia
  • L-type Ca channel
  • Nickel
  • TLR4

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Management, Monitoring, Policy and Law

Cite this

Involvement of L-type Ca2+ Channel and Toll-Like Receptor-4 in Nickel-Induced Interleukin-8 Gene Expression. / Lin, Chia Hsien; Chung, Chih Ang; Wong, Jhen Hong; Chen, Ben Kuen; Chiu, Siou Jin; Klahan, Sukhontip; Lee, Yi Chao; Chang, Wei-Chiao.

In: Environmental Toxicology, Vol. 31, No. 1, 01.01.2016, p. 5-12.

Research output: Contribution to journalArticle

Lin, Chia Hsien ; Chung, Chih Ang ; Wong, Jhen Hong ; Chen, Ben Kuen ; Chiu, Siou Jin ; Klahan, Sukhontip ; Lee, Yi Chao ; Chang, Wei-Chiao. / Involvement of L-type Ca2+ Channel and Toll-Like Receptor-4 in Nickel-Induced Interleukin-8 Gene Expression. In: Environmental Toxicology. 2016 ; Vol. 31, No. 1. pp. 5-12.
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