Involvement of heme oxygenase-1 participates in anti-inflammatory and analgesic effects of aqueous extract of hibiscus taiwanensis

Shu Ling Liu, Jeng Shyan Deng, Chuan Sung Chiu, Wen Chi Hou, Shyh Shyun Huang, Wang Ching Lin, Jung Chun Liao, Guan Jhong Huang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Anti-inflammatory effects of the aqueous extract of Hibiscus taiwanensis (AHT) were used in lipopolysaccharide (LPS-)stimulated mouse macrophage RAW264.7 cells and carrageenan (Carr-)induced mouse paw edema model. When RAW264.7 macrophages were treated with AHT together with LPS, a concentration-dependent inhibition of nitric oxide (NO), tumor necrosis factor (TNF-α), and prostaglandin E2 (PGE 2) levels productions were detected. Western blotting revealed that AHT blocked protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and elevated heme oxygenase-1 (HO-1), significantly. In the animal test, AHT decreased the paw edema at the 4th and the 5thh after Carr administration, and it increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the paw tissue. We also demonstrated AHT decreased the NO, TNF-, and PGE2 levels on the serum level at the 5th h after the Carr injection. Western blotting revealed that AHT decreased Carr-induced iNOS, and COX-2, and increased HO-1 expressions at the 5th h in the edema paw. These findings demonstrated that AHT has excellent anti-inflammatory activities in vitro and in vivo and thus it has great potential to be used as a source for natural health products.

Original languageEnglish
Article number132859
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012

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Hibiscus
Heme Oxygenase-1
Non-Steroidal Anti-Inflammatory Agents
Edema
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Dinoprostone
Nitric Oxide
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Western Blotting
Macrophages
Carrageenan
Glutathione Peroxidase
Catalase
Superoxide Dismutase
Lipopolysaccharides
Injections
Health
Serum

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Involvement of heme oxygenase-1 participates in anti-inflammatory and analgesic effects of aqueous extract of hibiscus taiwanensis. / Liu, Shu Ling; Deng, Jeng Shyan; Chiu, Chuan Sung; Hou, Wen Chi; Huang, Shyh Shyun; Lin, Wang Ching; Liao, Jung Chun; Huang, Guan Jhong.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 132859, 2012.

Research output: Contribution to journalArticle

Liu, Shu Ling ; Deng, Jeng Shyan ; Chiu, Chuan Sung ; Hou, Wen Chi ; Huang, Shyh Shyun ; Lin, Wang Ching ; Liao, Jung Chun ; Huang, Guan Jhong. / Involvement of heme oxygenase-1 participates in anti-inflammatory and analgesic effects of aqueous extract of hibiscus taiwanensis. In: Evidence-based Complementary and Alternative Medicine. 2012 ; Vol. 2012.
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