Involvement of F-actin in chaperonin-containing t-complex 1 beta regulating mouse mesangial cell functions in a glucose-induction cell model

Jin Shuen Chen, Li Chien Chang, Chia Chao Wu, Lai King Yeung, Yuh Feng Lin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of this study is to investigate the role of chaperonin-containing t-complex polypeptide 1 beta (CCT2) in the regulation of mouse mesangial cell (mMC) contraction, proliferation, and migration with filamentous/globular-(F/G-) actin ratio under high glucose induction. A low CCT2mMC model induced by treatment of small interference RNA was established. Groups with and without low CCT2 induction examined in normal and high (H) glucose conditions revealed the following major results: (1) low CCT2 or H glucose showed the ability to attenuate F/G-actin ratio; (2) groups with low F/G-actin ratio all showed less cell contraction; (3) suppression of CCT2 may reduce the proliferation and migration which were originally induced by H glucose. In conclusion, CCT2 can be used as a specific regulator for mMC contraction, proliferation, and migration affected by glucose, which mechanism may involve the alteration of F-actin, particularly for cell contraction.

Original languageEnglish
Article number565647
JournalExperimental Diabetes Research
Volume2011
DOIs
Publication statusPublished - 2011

Fingerprint

Chaperonins
Mesangial Cells
Actins
Chaperonin Containing TCP-1
Glucose
Cell Movement
Cell Proliferation
RNA Interference
Contraction
Induction
Mouse
Cells

ASJC Scopus subject areas

  • Medicine(all)
  • Endocrinology, Diabetes and Metabolism
  • Music

Cite this

Involvement of F-actin in chaperonin-containing t-complex 1 beta regulating mouse mesangial cell functions in a glucose-induction cell model. / Chen, Jin Shuen; Chang, Li Chien; Wu, Chia Chao; Yeung, Lai King; Lin, Yuh Feng.

In: Experimental Diabetes Research, Vol. 2011, 565647, 2011.

Research output: Contribution to journalArticle

@article{759b9664c5794a1f9bb41b7e0cc10d07,
title = "Involvement of F-actin in chaperonin-containing t-complex 1 beta regulating mouse mesangial cell functions in a glucose-induction cell model",
abstract = "The aim of this study is to investigate the role of chaperonin-containing t-complex polypeptide 1 beta (CCT2) in the regulation of mouse mesangial cell (mMC) contraction, proliferation, and migration with filamentous/globular-(F/G-) actin ratio under high glucose induction. A low CCT2mMC model induced by treatment of small interference RNA was established. Groups with and without low CCT2 induction examined in normal and high (H) glucose conditions revealed the following major results: (1) low CCT2 or H glucose showed the ability to attenuate F/G-actin ratio; (2) groups with low F/G-actin ratio all showed less cell contraction; (3) suppression of CCT2 may reduce the proliferation and migration which were originally induced by H glucose. In conclusion, CCT2 can be used as a specific regulator for mMC contraction, proliferation, and migration affected by glucose, which mechanism may involve the alteration of F-actin, particularly for cell contraction.",
author = "Chen, {Jin Shuen} and Chang, {Li Chien} and Wu, {Chia Chao} and Yeung, {Lai King} and Lin, {Yuh Feng}",
year = "2011",
doi = "10.1155/2011/565647",
language = "English",
volume = "2011",
journal = "Experimental Diabesity Research",
issn = "1543-8600",
publisher = "Taylor and Francis Ltd.",

}

TY - JOUR

T1 - Involvement of F-actin in chaperonin-containing t-complex 1 beta regulating mouse mesangial cell functions in a glucose-induction cell model

AU - Chen, Jin Shuen

AU - Chang, Li Chien

AU - Wu, Chia Chao

AU - Yeung, Lai King

AU - Lin, Yuh Feng

PY - 2011

Y1 - 2011

N2 - The aim of this study is to investigate the role of chaperonin-containing t-complex polypeptide 1 beta (CCT2) in the regulation of mouse mesangial cell (mMC) contraction, proliferation, and migration with filamentous/globular-(F/G-) actin ratio under high glucose induction. A low CCT2mMC model induced by treatment of small interference RNA was established. Groups with and without low CCT2 induction examined in normal and high (H) glucose conditions revealed the following major results: (1) low CCT2 or H glucose showed the ability to attenuate F/G-actin ratio; (2) groups with low F/G-actin ratio all showed less cell contraction; (3) suppression of CCT2 may reduce the proliferation and migration which were originally induced by H glucose. In conclusion, CCT2 can be used as a specific regulator for mMC contraction, proliferation, and migration affected by glucose, which mechanism may involve the alteration of F-actin, particularly for cell contraction.

AB - The aim of this study is to investigate the role of chaperonin-containing t-complex polypeptide 1 beta (CCT2) in the regulation of mouse mesangial cell (mMC) contraction, proliferation, and migration with filamentous/globular-(F/G-) actin ratio under high glucose induction. A low CCT2mMC model induced by treatment of small interference RNA was established. Groups with and without low CCT2 induction examined in normal and high (H) glucose conditions revealed the following major results: (1) low CCT2 or H glucose showed the ability to attenuate F/G-actin ratio; (2) groups with low F/G-actin ratio all showed less cell contraction; (3) suppression of CCT2 may reduce the proliferation and migration which were originally induced by H glucose. In conclusion, CCT2 can be used as a specific regulator for mMC contraction, proliferation, and migration affected by glucose, which mechanism may involve the alteration of F-actin, particularly for cell contraction.

UR - http://www.scopus.com/inward/record.url?scp=84555188226&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84555188226&partnerID=8YFLogxK

U2 - 10.1155/2011/565647

DO - 10.1155/2011/565647

M3 - Article

VL - 2011

JO - Experimental Diabesity Research

JF - Experimental Diabesity Research

SN - 1543-8600

M1 - 565647

ER -