Involvement of cholesterol in Campylobacter jejuni cytolethal distending toxin-induced pathogenesis

Cheng Kuo Lai, Jing Chi Su, Yu Hsin Lin, Chia Shuo Chang, Chun Lung Feng, Hwai Jeng Lin, Chun Jung Lin, Guan Chin Tseng, Hsin Ho Liu, Jer Tsong Hsieh, Hong Lin Su, Chih Ho Lai

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aim: The aim of this study was to investigate whether cholesterol plays a pivotal role in cytolethal distending toxin (CDT) mediated pathogenic effects in hosts. Materials & methods: The molecular mechanisms underlying cholesterol sequestering conferred resistance to CDT-induced DNA double-strand breaks (DSBs) and cell cycle arrest were investigated. Histopathological analysis was conducted for evaluating CDT-induced intestinal inflammation in mouse. Results: CDT actions were attenuated by treatment of cells with methyl-β-cyclodextrin (MβCD). Severe intestinal inflammation induced by CDT treatment was observed in high-cholesterol diet-fed mice, but not in normal diet-fed mice, indicating that cholesterol is essential for CDT intoxication. Conclusion: Our findings demonstrate a molecular link between Campylobacter jejuni CDT and cholesterol, which is crucial to facilitate CDT-induced pathogenesis in hosts.

Original languageEnglish
Pages (from-to)489-501
Number of pages13
JournalFuture Microbiology
Volume10
Issue number4
DOIs
Publication statusPublished - Apr 1 2015

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Campylobacter jejuni
Cholesterol
Diet
Inflammation
Double-Stranded DNA Breaks
cytolethal distending toxin
Cyclodextrins
Cell Cycle Checkpoints

Keywords

  • Campylobacter jejuni
  • cell cycle
  • cholesterol
  • cytolethal distending toxin
  • DNA double-strand breaks

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Involvement of cholesterol in Campylobacter jejuni cytolethal distending toxin-induced pathogenesis. / Lai, Cheng Kuo; Su, Jing Chi; Lin, Yu Hsin; Chang, Chia Shuo; Feng, Chun Lung; Lin, Hwai Jeng; Lin, Chun Jung; Tseng, Guan Chin; Liu, Hsin Ho; Hsieh, Jer Tsong; Su, Hong Lin; Lai, Chih Ho.

In: Future Microbiology, Vol. 10, No. 4, 01.04.2015, p. 489-501.

Research output: Contribution to journalArticle

Lai, CK, Su, JC, Lin, YH, Chang, CS, Feng, CL, Lin, HJ, Lin, CJ, Tseng, GC, Liu, HH, Hsieh, JT, Su, HL & Lai, CH 2015, 'Involvement of cholesterol in Campylobacter jejuni cytolethal distending toxin-induced pathogenesis', Future Microbiology, vol. 10, no. 4, pp. 489-501. https://doi.org/10.2217/fmb.14.119
Lai, Cheng Kuo ; Su, Jing Chi ; Lin, Yu Hsin ; Chang, Chia Shuo ; Feng, Chun Lung ; Lin, Hwai Jeng ; Lin, Chun Jung ; Tseng, Guan Chin ; Liu, Hsin Ho ; Hsieh, Jer Tsong ; Su, Hong Lin ; Lai, Chih Ho. / Involvement of cholesterol in Campylobacter jejuni cytolethal distending toxin-induced pathogenesis. In: Future Microbiology. 2015 ; Vol. 10, No. 4. pp. 489-501.
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