Investigation on liquid chromatographic separation of basic compounds using silica column with aqueous/organic mobile phase containing triethylamine and acetic acid

An-Bang Wu, Ming Chun Huang, Hsiu O. Ho, Geng Cheng Yeh, Ming Thau Sheu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A high-performance liquid chromatography (HPLC) method using silica column eluted with aqueous solvent mobile phase containing triethylamine (TEA) and acetic acid (ACH) at trace percentages was characterized for the analysis of basic compounds. The key mechanism of this system is ion-exchange accompanying interaction of silanol groups. The increase in the ACH concentration in the mobile phase minimizes the ionization of the silanol group, leading to reduced retention time. However, the greater extent of ionization of silanol caused by the increase of TEA concentration helps to retain basic compounds in the column. Further, the protonated TEA that is positively charged also competes for the ionized silanol group with basic compounds, resulting in the modification of retention time. On the other hand, the retention becomes longer with increasing proportion of either organic or aqueous solvent in mobile phase, and partial replacement of methanol with acetonitrile.

Original languageEnglish
Pages (from-to)443-449
Number of pages7
JournalBiomedical Chromatography
Volume18
Issue number7
DOIs
Publication statusPublished - Sep 2004

Fingerprint

Silicon Dioxide
Acetic Acid
Liquids
Ionization
Ion Exchange
Exchange interactions
High performance liquid chromatography
Methanol
Ion exchange
High Pressure Liquid Chromatography
triethylamine
silanol

Keywords

  • Acetic acid
  • Aqueous eluents
  • Basic compounds
  • Silica column
  • TH, tetrahydrozoline HC1
  • Triethylamine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

Cite this

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title = "Investigation on liquid chromatographic separation of basic compounds using silica column with aqueous/organic mobile phase containing triethylamine and acetic acid",
abstract = "A high-performance liquid chromatography (HPLC) method using silica column eluted with aqueous solvent mobile phase containing triethylamine (TEA) and acetic acid (ACH) at trace percentages was characterized for the analysis of basic compounds. The key mechanism of this system is ion-exchange accompanying interaction of silanol groups. The increase in the ACH concentration in the mobile phase minimizes the ionization of the silanol group, leading to reduced retention time. However, the greater extent of ionization of silanol caused by the increase of TEA concentration helps to retain basic compounds in the column. Further, the protonated TEA that is positively charged also competes for the ionized silanol group with basic compounds, resulting in the modification of retention time. On the other hand, the retention becomes longer with increasing proportion of either organic or aqueous solvent in mobile phase, and partial replacement of methanol with acetonitrile.",
keywords = "Acetic acid, Aqueous eluents, Basic compounds, Silica column, TH, tetrahydrozoline HC1, Triethylamine",
author = "An-Bang Wu and Huang, {Ming Chun} and Ho, {Hsiu O.} and Yeh, {Geng Cheng} and Sheu, {Ming Thau}",
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T1 - Investigation on liquid chromatographic separation of basic compounds using silica column with aqueous/organic mobile phase containing triethylamine and acetic acid

AU - Wu, An-Bang

AU - Huang, Ming Chun

AU - Ho, Hsiu O.

AU - Yeh, Geng Cheng

AU - Sheu, Ming Thau

PY - 2004/9

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N2 - A high-performance liquid chromatography (HPLC) method using silica column eluted with aqueous solvent mobile phase containing triethylamine (TEA) and acetic acid (ACH) at trace percentages was characterized for the analysis of basic compounds. The key mechanism of this system is ion-exchange accompanying interaction of silanol groups. The increase in the ACH concentration in the mobile phase minimizes the ionization of the silanol group, leading to reduced retention time. However, the greater extent of ionization of silanol caused by the increase of TEA concentration helps to retain basic compounds in the column. Further, the protonated TEA that is positively charged also competes for the ionized silanol group with basic compounds, resulting in the modification of retention time. On the other hand, the retention becomes longer with increasing proportion of either organic or aqueous solvent in mobile phase, and partial replacement of methanol with acetonitrile.

AB - A high-performance liquid chromatography (HPLC) method using silica column eluted with aqueous solvent mobile phase containing triethylamine (TEA) and acetic acid (ACH) at trace percentages was characterized for the analysis of basic compounds. The key mechanism of this system is ion-exchange accompanying interaction of silanol groups. The increase in the ACH concentration in the mobile phase minimizes the ionization of the silanol group, leading to reduced retention time. However, the greater extent of ionization of silanol caused by the increase of TEA concentration helps to retain basic compounds in the column. Further, the protonated TEA that is positively charged also competes for the ionized silanol group with basic compounds, resulting in the modification of retention time. On the other hand, the retention becomes longer with increasing proportion of either organic or aqueous solvent in mobile phase, and partial replacement of methanol with acetonitrile.

KW - Acetic acid

KW - Aqueous eluents

KW - Basic compounds

KW - Silica column

KW - TH, tetrahydrozoline HC1

KW - Triethylamine

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