Investigation of the percutaneous penetration of prostaglandin E1 and its ethyl ester

Ming Thau Sheu, Lun Huei Lin, Brend W. Spur, Patrick Y K Wong, Han Sun Chiang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The optimization of percutaneous delivery of PGE1 and its ethyl ester in alcoholic buffer solution through hairless mouse skin was investigated. A reversed-phase HPLC system with a photodiode array detector was used to differentiate the UV spectra of the penetration products. By comparison of the UV spectrum for each chromatographic peak, the conversion of PGE1 ethyl ester to PGE1 by enzymatic hydrolysis was found to be the predominant degradation pathway during the in vitro penetration. The quantification of ethyl ester was developed based on the same principle as that for PGE1. It was then applied to monitor the penetration of prostaglandins through hairless mouse skin from the vehicles containing various fractions of alcohol. Results demonstrated that the alkyl group promoted the penetration mainly as a result of enhancing the drug partitioning into the stratum corneum at its maximal thermodynamic activity. The alcohol fraction around 20% seemed to be optimal for the percutaneous delivery of the ethyl ester. The use of collagen gel to carry PGE1 ethyl ester for percutaneous application was included for comparison. The effect of adding alcohol in the collagen gel on the penetration of PGE1 ethyl ester was found to be slightly lower than that from the same vehicle without collagen. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)153-160
Number of pages8
JournalJournal of Controlled Release
Volume55
Issue number2-3
DOIs
Publication statusPublished - Nov 13 1998

Fingerprint

Hairless Mouse
Collagen
Alprostadil
Alcohols
Esters
Gels
Skin
Thermodynamics
Cornea
Prostaglandins
Buffers
Hydrolysis
High Pressure Liquid Chromatography
prostaglandin E1 ethyl ester
Pharmaceutical Preparations
In Vitro Techniques

Keywords

  • Collagen gel
  • Cosolvent
  • Ethyl ester
  • Penetration
  • Prostaglandin E

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Investigation of the percutaneous penetration of prostaglandin E1 and its ethyl ester. / Sheu, Ming Thau; Lin, Lun Huei; Spur, Brend W.; Wong, Patrick Y K; Chiang, Han Sun.

In: Journal of Controlled Release, Vol. 55, No. 2-3, 13.11.1998, p. 153-160.

Research output: Contribution to journalArticle

Sheu, Ming Thau ; Lin, Lun Huei ; Spur, Brend W. ; Wong, Patrick Y K ; Chiang, Han Sun. / Investigation of the percutaneous penetration of prostaglandin E1 and its ethyl ester. In: Journal of Controlled Release. 1998 ; Vol. 55, No. 2-3. pp. 153-160.
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