Inversion/Dimerization of plasmids mediated by inverted repeats

Yi Lisa Lyu, Ching Tai Lin, Leroy-Fong Liu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In contrast with earlier studies on the lambda and Escherichia coli genomes, recombination between inverted repeats on plasmids is highly efficient and shown to be recA-independent. In addition, the recombination product is exclusively a head-to-head inverted dimer. Here, we show that this recombination/rearrangement event can occur on different plasmid replicons and is not specific to the particular sequence within the inverted repeats. Transcription readthrough into the inverted repeats has little effect on this event. Genetic analysis has also indicated that most known recombination enzymes are not involved in this process. Specifically, single or double mutants defective in Holliday junction resolution systems (RuvABC and/or RecG/RusA) do not abolish this recombination/rearrangement event. This result does not support the previous models (i.e. the reciprocal-strand-switching and the cruciform-dumbbell models) in which intermediates containing Holliday junctions are proposed. Further analysis has demonstrated that the recombination/ rearrangement frequency is dramatically (over 1000-fold) reduced if mismatches (2.8%) are present within the inverted repeats. Mutations in dam, mutH and mutL genes partially or completely restored the recombination/rearrangement frequency to the level exhibited by the perfect inverted repeats, suggesting the formation of heteroduplexes during recombination/rearrangement. Sequencing analysis of the recombination/rearrangement products have indicated that the majority of the products do not involve crossing-over. We discuss a possible mechanism in which blockage of the lagging strand polymerase by a hairpin triggers recombination/rearrangement mediated by inverted repeats.

Original languageEnglish
Pages (from-to)1485-1501
Number of pages17
JournalJournal of Molecular Biology
Volume285
Issue number4
DOIs
Publication statusPublished - Jan 29 1999
Externally publishedYes

Fingerprint

Dimerization
Genetic Recombination
Plasmids
Cruciform DNA
Inverted Repeat Sequences
Replicon
Genome
Escherichia coli
Mutation

Keywords

  • Dumbbell intermediate
  • Gene amplification
  • Holliday junction
  • Inverted repeats
  • Mismatch repair

ASJC Scopus subject areas

  • Virology

Cite this

Inversion/Dimerization of plasmids mediated by inverted repeats. / Lyu, Yi Lisa; Lin, Ching Tai; Liu, Leroy-Fong.

In: Journal of Molecular Biology, Vol. 285, No. 4, 29.01.1999, p. 1485-1501.

Research output: Contribution to journalArticle

Lyu, Yi Lisa ; Lin, Ching Tai ; Liu, Leroy-Fong. / Inversion/Dimerization of plasmids mediated by inverted repeats. In: Journal of Molecular Biology. 1999 ; Vol. 285, No. 4. pp. 1485-1501.
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