Intravesical delivery of 5-aminolevulinic acid from water-in-oil nano/submicron-emulsion systems

Jia You Fang, Pao Chu Wu, Chia Lang Fang, Chao Huang Chen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The present work reports on the development of water-in-oil (w/o) emulsions for the intravesical administration of 5-aminolevulinic acid (ALA). The physicochemical properties of droplet size, zeta potential, and viscosity of the emulsions are characterized and the ability of the emulsions to release ALA following in vitro application is tested. The delivery systems are administered intravesically for 1 and 3 h in rats to examine the drug accumulation in bladder tissue. The mean size and zeta potential of the emulsions are 50-200nm and -3 to -14 mV, respectively. The loading of ALA into the emulsions resulted in a slower and sustained release. The release extent was found to be inversely related to the droplet size of the emulsions. The emulsions did not increase the drug permeation into tissues during short exposure duration (1 h). When the dwell time was extended to 3 h, the systems showed a 2.7-fold increase in the ALA concentration in the bladder wall. Images of confocal laser scanning microscopy demonstrated a higher and deeper fluorescence signal, with emulsion administration, as compared to the aqueous control. Intravesical emulsion delivery provides a significant advantage for drugs targeting bladder tissues.

Original languageEnglish
Pages (from-to)2375-2385
Number of pages11
JournalJournal of Pharmaceutical Sciences
Volume99
Issue number5
DOIs
Publication statusPublished - May 2010

Fingerprint

Aminolevulinic Acid
Emulsions
Oils
Water
Urinary Bladder
Intravesical Administration
Drug Delivery Systems
Confocal Microscopy
Viscosity
Pharmaceutical Preparations
Fluorescence

Keywords

  • 5-aminolevulinic acid
  • Bladder
  • Emulsion
  • Intravesical delivery
  • Sustained release

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Intravesical delivery of 5-aminolevulinic acid from water-in-oil nano/submicron-emulsion systems. / Fang, Jia You; Wu, Pao Chu; Fang, Chia Lang; Chen, Chao Huang.

In: Journal of Pharmaceutical Sciences, Vol. 99, No. 5, 05.2010, p. 2375-2385.

Research output: Contribution to journalArticle

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