Intravascular infusion of acid promotes intrapulmonary inducible nitric oxide synthase activity and impairs blood oxygenation in rats

Ikram U. Haque, Chun Jen Huang, Philip O. Scumpia, Omer Nasiroglu, Jeffrey W. Skimming

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: To test the hypothesis that intravascular acid infusion promotes intrapulmonary nitric oxide formation by promoting inducible nitric oxide synthase (iNOS) and inhibiting endothelial nitric oxide synthase (eNOS) expression in rats. Design: Prospective, placebo controlled, randomized laboratory study. Setting: University laboratory. Subjects: Twelve male Sprague-Dawley rats weighing 317 ± 30 g served as study subjects. All animals were anesthetized, paralyzed, and mechanically ventilated throughout the experiment. Interventions: The animals were randomized to receive either 0.1 N hydrochloric acid or 0.9% saline intravenously. The infusions were initially given at a rate of 11 mL/kg/hr for 15 mins and then at a rate of 0.95 mL/kg/hr for the remainder of the experiment. Exhaled nitric oxide concentrations and hemodynamic measurements were monitored throughout the experiment. Lung tissues were harvested for Western blot analysis and immunostaining 4 hrs after starting the intravascular infusion. Measurement and Main Results: At the end of the experiment, we found more than a four-fold higher concentration of exhaled nitric oxide in the acid-treated animals than in the saline-treated animals (p < .001). Western blot analysis revealed that the acid infusion increased intrapulmonary iNOS concentrations (p < .001), yet it decreased intrapulmonary eNOS concentrations (p = .009). Acid-related lung injury manifested as a decrease in blood oxygen tensions (p = .045) and as an increase in lung homogenate interleukin-6 concentrations (p = .003). Conclusions: Our results reveal that hydrochloric acid infusion stimulates intrapulmonary nitric oxide formation at least in part by promoting the expression of iNOS. Our findings suggest that correcting acidosis should attenuate iNOS formation. Our data also support the idea that metabolic acidosis itself can lead to impaired intrapulmonary gas exchange and increased expression of pro-inflammatory cytokines such as interleukin-6. Whether the induction of intrapulmonary nitric oxide formation mediates or simply indicates lung injury warrants further investigation.

Original languageEnglish
Pages (from-to)1454-1460
Number of pages7
JournalCritical Care Medicine
Volume31
Issue number5
DOIs
Publication statusPublished - May 1 2003
Externally publishedYes

Fingerprint

Nitric Oxide Synthase Type II
Nitric Oxide
Acids
Hydrochloric Acid
Nitric Oxide Synthase Type III
Lung Injury
Acidosis
Interleukin-6
Western Blotting
Lung
Sprague Dawley Rats
Gases
Hemodynamics
Placebos
Cytokines
Oxygen

Keywords

  • Acidosis
  • Interleukin-6
  • Lung
  • Lung diseases
  • Nitric oxide
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Intravascular infusion of acid promotes intrapulmonary inducible nitric oxide synthase activity and impairs blood oxygenation in rats. / Haque, Ikram U.; Huang, Chun Jen; Scumpia, Philip O.; Nasiroglu, Omer; Skimming, Jeffrey W.

In: Critical Care Medicine, Vol. 31, No. 5, 01.05.2003, p. 1454-1460.

Research output: Contribution to journalArticle

Haque, Ikram U. ; Huang, Chun Jen ; Scumpia, Philip O. ; Nasiroglu, Omer ; Skimming, Jeffrey W. / Intravascular infusion of acid promotes intrapulmonary inducible nitric oxide synthase activity and impairs blood oxygenation in rats. In: Critical Care Medicine. 2003 ; Vol. 31, No. 5. pp. 1454-1460.
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AU - Haque, Ikram U.

AU - Huang, Chun Jen

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AU - Skimming, Jeffrey W.

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N2 - Objective: To test the hypothesis that intravascular acid infusion promotes intrapulmonary nitric oxide formation by promoting inducible nitric oxide synthase (iNOS) and inhibiting endothelial nitric oxide synthase (eNOS) expression in rats. Design: Prospective, placebo controlled, randomized laboratory study. Setting: University laboratory. Subjects: Twelve male Sprague-Dawley rats weighing 317 ± 30 g served as study subjects. All animals were anesthetized, paralyzed, and mechanically ventilated throughout the experiment. Interventions: The animals were randomized to receive either 0.1 N hydrochloric acid or 0.9% saline intravenously. The infusions were initially given at a rate of 11 mL/kg/hr for 15 mins and then at a rate of 0.95 mL/kg/hr for the remainder of the experiment. Exhaled nitric oxide concentrations and hemodynamic measurements were monitored throughout the experiment. Lung tissues were harvested for Western blot analysis and immunostaining 4 hrs after starting the intravascular infusion. Measurement and Main Results: At the end of the experiment, we found more than a four-fold higher concentration of exhaled nitric oxide in the acid-treated animals than in the saline-treated animals (p < .001). Western blot analysis revealed that the acid infusion increased intrapulmonary iNOS concentrations (p < .001), yet it decreased intrapulmonary eNOS concentrations (p = .009). Acid-related lung injury manifested as a decrease in blood oxygen tensions (p = .045) and as an increase in lung homogenate interleukin-6 concentrations (p = .003). Conclusions: Our results reveal that hydrochloric acid infusion stimulates intrapulmonary nitric oxide formation at least in part by promoting the expression of iNOS. Our findings suggest that correcting acidosis should attenuate iNOS formation. Our data also support the idea that metabolic acidosis itself can lead to impaired intrapulmonary gas exchange and increased expression of pro-inflammatory cytokines such as interleukin-6. Whether the induction of intrapulmonary nitric oxide formation mediates or simply indicates lung injury warrants further investigation.

AB - Objective: To test the hypothesis that intravascular acid infusion promotes intrapulmonary nitric oxide formation by promoting inducible nitric oxide synthase (iNOS) and inhibiting endothelial nitric oxide synthase (eNOS) expression in rats. Design: Prospective, placebo controlled, randomized laboratory study. Setting: University laboratory. Subjects: Twelve male Sprague-Dawley rats weighing 317 ± 30 g served as study subjects. All animals were anesthetized, paralyzed, and mechanically ventilated throughout the experiment. Interventions: The animals were randomized to receive either 0.1 N hydrochloric acid or 0.9% saline intravenously. The infusions were initially given at a rate of 11 mL/kg/hr for 15 mins and then at a rate of 0.95 mL/kg/hr for the remainder of the experiment. Exhaled nitric oxide concentrations and hemodynamic measurements were monitored throughout the experiment. Lung tissues were harvested for Western blot analysis and immunostaining 4 hrs after starting the intravascular infusion. Measurement and Main Results: At the end of the experiment, we found more than a four-fold higher concentration of exhaled nitric oxide in the acid-treated animals than in the saline-treated animals (p < .001). Western blot analysis revealed that the acid infusion increased intrapulmonary iNOS concentrations (p < .001), yet it decreased intrapulmonary eNOS concentrations (p = .009). Acid-related lung injury manifested as a decrease in blood oxygen tensions (p = .045) and as an increase in lung homogenate interleukin-6 concentrations (p = .003). Conclusions: Our results reveal that hydrochloric acid infusion stimulates intrapulmonary nitric oxide formation at least in part by promoting the expression of iNOS. Our findings suggest that correcting acidosis should attenuate iNOS formation. Our data also support the idea that metabolic acidosis itself can lead to impaired intrapulmonary gas exchange and increased expression of pro-inflammatory cytokines such as interleukin-6. Whether the induction of intrapulmonary nitric oxide formation mediates or simply indicates lung injury warrants further investigation.

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KW - Interleukin-6

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KW - Lung diseases

KW - Nitric oxide

KW - Nitric oxide synthase

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