Intratypic recombination among lineages of type 1 vaccine-derived poliovirus emerging during chronic infection of an immunodeficient patient

Chen Fu Yang, Hour Young Chen, Jaume Jorba, Hui Chih Sun, Su Ju Yang, Hsiang Chi Lee, Yhu Chering Huang, Tzou Yien Lin, Pei Jer Chen, Hiroyuki Shimizu, Yorihiro Nishimura, Andi Utama, Mark Pallansch, Tatsuo Miyamura, Olen Kew, Jyh Yuan Yang

Research output: Contribution to journalArticle

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Abstract

We determined the complete genomic sequences of nine type 1 immunodeficient vaccine-derived poliovirus (iVDPV) isolates obtained over a 337-day period from a poliomyelitis patient from Taiwan with common variable immunodeficiency. The iVDPV isolates differed from the Sabin type 1 oral poliovirus vaccine (OPV) strain at 1.84% to 3.15% of total open reading frame positions and had diverged into at least five distinct lineages. Phylogenetic analysis suggested that the chronic infection was initiated by the fifth and last OPV dose, given 567 days before onset of paralysis, and that divergence of major lineages began very early in the chronic infection. Key determinants of attenuation in Sabin 1 had reverted in the iVDPV isolates, and representative isolates of each lineage showed increased neurovirulence for PVR-Tg21 transgenic mice. None of the isolates had retained the temperature-sensitive phenotype of Sabin 1. All isolates were antigenic variants of Sabin 1, having multiple amino acid substitutions within or near neutralizing antigenic sites 1, 2, and 3a. Antigenic divergence of the iVDPV variants from Sabin 1 followed two major independent evolutionary pathways. The emergence of distinct coreplicating lineages suggests that iVDPVs can replicate for many months at separate sites in the gastrointestinal tract. Some isolates had mosaic genome structures indicative of recombination across and within lineages. iVDPV excretion apparently ceased after 30 to 35 months of chronic infection. The appearance of a chronic VDPV excretor in a tropical, developing country has important implications for the strategy to stop OPV immunization after eradication of wild polioviruses.

Original languageEnglish
Pages (from-to)12623-12634
Number of pages12
JournalJournal of Virology
Volume79
Issue number20
DOIs
Publication statusPublished - 2005
Externally publishedYes

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Poliovirus Vaccines
Enterovirus C
Genetic Recombination
Oral Poliovirus Vaccine
vaccines
Infection
infection
mouth
Common Variable Immunodeficiency
Poliovirus
Poliomyelitis
Amino Acid Substitution
Taiwan
Paralysis
Transgenic Mice
Developing Countries
Open Reading Frames
Gastrointestinal Tract
Immunization
Genome

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Intratypic recombination among lineages of type 1 vaccine-derived poliovirus emerging during chronic infection of an immunodeficient patient. / Yang, Chen Fu; Chen, Hour Young; Jorba, Jaume; Sun, Hui Chih; Yang, Su Ju; Lee, Hsiang Chi; Huang, Yhu Chering; Lin, Tzou Yien; Chen, Pei Jer; Shimizu, Hiroyuki; Nishimura, Yorihiro; Utama, Andi; Pallansch, Mark; Miyamura, Tatsuo; Kew, Olen; Yang, Jyh Yuan.

In: Journal of Virology, Vol. 79, No. 20, 2005, p. 12623-12634.

Research output: Contribution to journalArticle

Yang, CF, Chen, HY, Jorba, J, Sun, HC, Yang, SJ, Lee, HC, Huang, YC, Lin, TY, Chen, PJ, Shimizu, H, Nishimura, Y, Utama, A, Pallansch, M, Miyamura, T, Kew, O & Yang, JY 2005, 'Intratypic recombination among lineages of type 1 vaccine-derived poliovirus emerging during chronic infection of an immunodeficient patient', Journal of Virology, vol. 79, no. 20, pp. 12623-12634. https://doi.org/10.1128/JVI.79.20.12623-12634.2005
Yang, Chen Fu ; Chen, Hour Young ; Jorba, Jaume ; Sun, Hui Chih ; Yang, Su Ju ; Lee, Hsiang Chi ; Huang, Yhu Chering ; Lin, Tzou Yien ; Chen, Pei Jer ; Shimizu, Hiroyuki ; Nishimura, Yorihiro ; Utama, Andi ; Pallansch, Mark ; Miyamura, Tatsuo ; Kew, Olen ; Yang, Jyh Yuan. / Intratypic recombination among lineages of type 1 vaccine-derived poliovirus emerging during chronic infection of an immunodeficient patient. In: Journal of Virology. 2005 ; Vol. 79, No. 20. pp. 12623-12634.
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AU - Lee, Hsiang Chi

AU - Huang, Yhu Chering

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AU - Chen, Pei Jer

AU - Shimizu, Hiroyuki

AU - Nishimura, Yorihiro

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