Intrathecal miR-183 delivery suppresses mechanical allodynia in mononeuropathic rats

Chung Ren Lin, Kuan Hung Chen, Chien Hui Yang, Hui Wen Huang, Shyr Ming Sheen-Chen

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Members of the miR-183 family are unique in that they are highly abundant in sensory organs. In a recent study, significant downregulation was observed for miR-96 and miR-183 in the L5 dorsal root ganglion (DRG) 2 weeks after spinal nerve ligation (SNL). In this study, we focused on miR-183, which is the most regulated member of the miR-183 family, to look at the specific role on neuropathic pain. Persistent mechanical allodynia was induced with the L5 SNL model in 8-week-old male Sprague-Dawley rats. Paw withdrawal thresholds in response to mechanical stimuli were assessed with Von Frey filaments. Expression of miR-183 in the L5 DRG was assessed with quantitative real-time polymerase chain reaction (qPCR) analysis. Lentivirions expressing miR-183 were injected intrathecally into SNL rats. Changes in mechanical allodynia were assessed with Von Frey filaments. In addition, changes in the predicted target genes of miR-183 were assessed with qPCR. L5 SNL produced marked mechanical allodynia in the ipsilateral hindpaws of adult rats, beginning at postoperative day 1 and continuing to day 14. L5 SNL caused significant downregulation of miR-183 in adult DRG cells. Intrathecal administration of lentivirions expressing miR-183 downregulated SNL-induced increases in the expression of Nav1.3 and brain-derived neurotrophic factor (BDNF), which correlated with the significant attenuation of SNL-induced mechanical allodynia.

Original languageEnglish
Pages (from-to)1682-1689
Number of pages8
JournalEuropean Journal of Neuroscience
Volume39
Issue number10
DOIs
Publication statusPublished - May 2014
Externally publishedYes

Fingerprint

Spinal Nerves
Hyperalgesia
Ligation
Spinal Ganglia
Down-Regulation
Real-Time Polymerase Chain Reaction
Brain-Derived Neurotrophic Factor
Neuralgia
Sprague Dawley Rats
Genes

Keywords

  • Intrathecal delivery
  • Lentivirions
  • Neuropathic pain
  • Spinal nerve ligation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Intrathecal miR-183 delivery suppresses mechanical allodynia in mononeuropathic rats. / Lin, Chung Ren; Chen, Kuan Hung; Yang, Chien Hui; Huang, Hui Wen; Sheen-Chen, Shyr Ming.

In: European Journal of Neuroscience, Vol. 39, No. 10, 05.2014, p. 1682-1689.

Research output: Contribution to journalArticle

Lin, Chung Ren ; Chen, Kuan Hung ; Yang, Chien Hui ; Huang, Hui Wen ; Sheen-Chen, Shyr Ming. / Intrathecal miR-183 delivery suppresses mechanical allodynia in mononeuropathic rats. In: European Journal of Neuroscience. 2014 ; Vol. 39, No. 10. pp. 1682-1689.
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