Intraportal injection of insulin-producing cells generated from human bone marrow mesenchymal stem cells decreases blood glucose level in diabetic rats

Pei Jiun Tsai, Hwai Shi Wang, Chi Hung Lin, Zen Chung Weng, Tien Hua Chen, Jia Fwu Shyu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We studied the process of trans-differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs) into insulin-producing cells. Streptozotocin (STZ)-induced diabetic rat model was used to study the effect of portal vein transplantation of these insulin-producing cells on blood sugar levels. The BM-MSCs were differentiated into insulin-producing cells under defined conditions. Real-time PCR, immunocytochemistry and glucose challenge were used to evaluate in vitro differentiation. Flow cytometry showed that hBM-MSCs were strongly positive for CD44, CD105 and CD73 and negative for hematopoietic markers CD34, CD38 and CD45. Differentiated cells expressed C-peptide as well as β-cells specific genes and hormones. Glucose stimulation increased C-peptide secretion in these cells. The insulin-producing, differentiated cells were transplanted into the portal vein of STZ-induced diabetic rats using a Port-A catheter. The insulin-producing cells were localized in the liver of the recipient rat and expressed human C-peptide. Blood glucose levels were reduced in diabetic rats transplanted with insulin-producing cells. We concluded that hBM-MSCs could be trans-differentiated into insulin-producing cells in vitro. Portal vein transplantation of insulin-producing cells alleviated hyperglycemia in diabetic rats.

Original languageEnglish
Pages (from-to)26-33
Number of pages8
JournalEndocrine Research
Volume39
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Mesenchymal Stromal Cells
Blood Glucose
Bone Marrow
Insulin
Injections
C-Peptide
Portal Vein
Streptozocin
Transplantation
Glucose
Vascular Access Devices
Hyperglycemia
Real-Time Polymerase Chain Reaction
Flow Cytometry
Immunohistochemistry
Hormones
Liver

Keywords

  • Diabetes
  • Human bone marrow
  • Insulin-producing cells
  • Mesenchymal stem cells
  • Transplantation

ASJC Scopus subject areas

  • Endocrinology

Cite this

Intraportal injection of insulin-producing cells generated from human bone marrow mesenchymal stem cells decreases blood glucose level in diabetic rats. / Tsai, Pei Jiun; Wang, Hwai Shi; Lin, Chi Hung; Weng, Zen Chung; Chen, Tien Hua; Shyu, Jia Fwu.

In: Endocrine Research, Vol. 39, No. 1, 2014, p. 26-33.

Research output: Contribution to journalArticle

Tsai, Pei Jiun ; Wang, Hwai Shi ; Lin, Chi Hung ; Weng, Zen Chung ; Chen, Tien Hua ; Shyu, Jia Fwu. / Intraportal injection of insulin-producing cells generated from human bone marrow mesenchymal stem cells decreases blood glucose level in diabetic rats. In: Endocrine Research. 2014 ; Vol. 39, No. 1. pp. 26-33.
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