Intrapersonal mutation of rmpA and rmpA2: A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates

Wen Liang Yu, Mei Feng Lee, Ming Chung Chang, Yin Ching Chuang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE). However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. A previously unrelated liver abscess K. pneumoniae hypermucoviscous isolate was used as a control. PCR, DNA cloning and sequencing for the plasmid-borne rmpA and rmpA2 genes and the chromosome-borne rmpA gene (c-rmpA) revealed negative c-rmpA with natural frame-shift mutation of rmpA and rmpA2 genes in the urine strain. The blood strain was negative for c-rmpA with rmpA2 mutation but no mutation in rmpA. The control strain was positive for c-rmpA with rmpA2 mutation but no mutation in rmpA and showed the highest virulence in mouse lethality experiments [median lethal dose (LD50) = 50 CFU], which was followed by the blood strain (LD50 = 2.47 × 103 CFU) and the urine strain (LD50 > 107 CFU). The control and blood strains were highly serum resistant, whereas the urine strain was sensitive to serum killing. In conclusion, intrapersonal concurrent mutation of rmpA and rmpA2 genes in the absence of c-rmpA could be a reason for the negative hypermucoviscosity phenotype and low virulence in rmpA-positive K. pneumoniae.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalJournal of Global Antimicrobial Resistance
Volume3
Issue number2
DOIs
Publication statusPublished - 2015

Fingerprint

Klebsiella pneumoniae
Virulence
Phenotype
Mutation
Lethal Dose 50
Chromosomes
Genes
Urine
Liver Abscess
Frameshift Mutation
Pulsed Field Gel Electrophoresis
Serum
DNA Sequence Analysis
Organism Cloning
Plasmids
Polymerase Chain Reaction

Keywords

  • Hypermucoviscosity
  • rmpA gene
  • Spontaneous mutation
  • Virulence

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Intrapersonal mutation of rmpA and rmpA2 : A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates. / Yu, Wen Liang; Lee, Mei Feng; Chang, Ming Chung; Chuang, Yin Ching.

In: Journal of Global Antimicrobial Resistance, Vol. 3, No. 2, 2015, p. 137-141.

Research output: Contribution to journalArticle

@article{f1f26340ef134554960a7295da7988ec,
title = "Intrapersonal mutation of rmpA and rmpA2: A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates",
abstract = "Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE). However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. A previously unrelated liver abscess K. pneumoniae hypermucoviscous isolate was used as a control. PCR, DNA cloning and sequencing for the plasmid-borne rmpA and rmpA2 genes and the chromosome-borne rmpA gene (c-rmpA) revealed negative c-rmpA with natural frame-shift mutation of rmpA and rmpA2 genes in the urine strain. The blood strain was negative for c-rmpA with rmpA2 mutation but no mutation in rmpA. The control strain was positive for c-rmpA with rmpA2 mutation but no mutation in rmpA and showed the highest virulence in mouse lethality experiments [median lethal dose (LD50) = 50 CFU], which was followed by the blood strain (LD50 = 2.47 × 103 CFU) and the urine strain (LD50 > 107 CFU). The control and blood strains were highly serum resistant, whereas the urine strain was sensitive to serum killing. In conclusion, intrapersonal concurrent mutation of rmpA and rmpA2 genes in the absence of c-rmpA could be a reason for the negative hypermucoviscosity phenotype and low virulence in rmpA-positive K. pneumoniae.",
keywords = "Hypermucoviscosity, rmpA gene, Spontaneous mutation, Virulence",
author = "Yu, {Wen Liang} and Lee, {Mei Feng} and Chang, {Ming Chung} and Chuang, {Yin Ching}",
year = "2015",
doi = "10.1016/j.jgar.2015.03.008",
language = "English",
volume = "3",
pages = "137--141",
journal = "Journal of Global Antimicrobial Resistance",
issn = "2213-7165",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Intrapersonal mutation of rmpA and rmpA2

T2 - A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates

AU - Yu, Wen Liang

AU - Lee, Mei Feng

AU - Chang, Ming Chung

AU - Chuang, Yin Ching

PY - 2015

Y1 - 2015

N2 - Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE). However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. A previously unrelated liver abscess K. pneumoniae hypermucoviscous isolate was used as a control. PCR, DNA cloning and sequencing for the plasmid-borne rmpA and rmpA2 genes and the chromosome-borne rmpA gene (c-rmpA) revealed negative c-rmpA with natural frame-shift mutation of rmpA and rmpA2 genes in the urine strain. The blood strain was negative for c-rmpA with rmpA2 mutation but no mutation in rmpA. The control strain was positive for c-rmpA with rmpA2 mutation but no mutation in rmpA and showed the highest virulence in mouse lethality experiments [median lethal dose (LD50) = 50 CFU], which was followed by the blood strain (LD50 = 2.47 × 103 CFU) and the urine strain (LD50 > 107 CFU). The control and blood strains were highly serum resistant, whereas the urine strain was sensitive to serum killing. In conclusion, intrapersonal concurrent mutation of rmpA and rmpA2 genes in the absence of c-rmpA could be a reason for the negative hypermucoviscosity phenotype and low virulence in rmpA-positive K. pneumoniae.

AB - Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE). However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. A previously unrelated liver abscess K. pneumoniae hypermucoviscous isolate was used as a control. PCR, DNA cloning and sequencing for the plasmid-borne rmpA and rmpA2 genes and the chromosome-borne rmpA gene (c-rmpA) revealed negative c-rmpA with natural frame-shift mutation of rmpA and rmpA2 genes in the urine strain. The blood strain was negative for c-rmpA with rmpA2 mutation but no mutation in rmpA. The control strain was positive for c-rmpA with rmpA2 mutation but no mutation in rmpA and showed the highest virulence in mouse lethality experiments [median lethal dose (LD50) = 50 CFU], which was followed by the blood strain (LD50 = 2.47 × 103 CFU) and the urine strain (LD50 > 107 CFU). The control and blood strains were highly serum resistant, whereas the urine strain was sensitive to serum killing. In conclusion, intrapersonal concurrent mutation of rmpA and rmpA2 genes in the absence of c-rmpA could be a reason for the negative hypermucoviscosity phenotype and low virulence in rmpA-positive K. pneumoniae.

KW - Hypermucoviscosity

KW - rmpA gene

KW - Spontaneous mutation

KW - Virulence

UR - http://www.scopus.com/inward/record.url?scp=84947017650&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947017650&partnerID=8YFLogxK

U2 - 10.1016/j.jgar.2015.03.008

DO - 10.1016/j.jgar.2015.03.008

M3 - Article

AN - SCOPUS:84947017650

VL - 3

SP - 137

EP - 141

JO - Journal of Global Antimicrobial Resistance

JF - Journal of Global Antimicrobial Resistance

SN - 2213-7165

IS - 2

ER -