Intranasal delivery of mannan-coated liposomes enhances antibody responses to a newcastle disease virus vaccine

Li Ping Tseng, Chwei Jang Chiou, Ming Chung Deng, Yi You Huang, Der Zen Liu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The polysaccharide, mannan, has been shown to be an effective immunoprotective agent and vaccine adjuvant in a variety of bacterial and viral diseases in different animal species. The objective of this study was to evaluate the immune response of chickens after being intranasal (i.n.) immunized using the Newcastle disease virus (NDV) encapsulated in liposomes and coated with mannan. Groups of chickens were vaccinated once intranasally with virus alone, then with uncoated liposome-encapsulated NDV (NC-lip), or mannan-coated liposome-encapsulated NDV (Man-lip). On day 14, velogenic viral challenge was carried out on vaccinated chickens. Chickens receiving virus alone had a 20% survival rate. In contrast, groups that received Man-lip had a higher survival rate of 70% (P <0.01). The titers of mucosal secretary immunoglobulin A (s-IgA) was the highest in the group for the birds that received Man-lip as mucosal adjuvant (P <0.01). These results suggest that liposomes coated with mannan may thus be used as a potential adjuvant for mucosal vaccination against NDV. This is the first study in which mannan has been demonstrated to enhance an adaptive immune response against ND virus infection in chickens.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalBiomedical Engineering - Applications, Basis and Communications
Volume22
Issue number1
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Mannans
Newcastle disease virus
Vaccines
Liposomes
Viruses
Antibodies
Antibody Formation
Chickens
Virus Diseases
Birds
Adaptive Immunity
Polysaccharides
Lip
Immunoglobulin A
Vaccination
Animals

Keywords

  • Adjuvant
  • Intranasal
  • Mannan-liposomes
  • Newcastle disease virus

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Bioengineering

Cite this

Intranasal delivery of mannan-coated liposomes enhances antibody responses to a newcastle disease virus vaccine. / Tseng, Li Ping; Chiou, Chwei Jang; Deng, Ming Chung; Huang, Yi You; Liu, Der Zen.

In: Biomedical Engineering - Applications, Basis and Communications, Vol. 22, No. 1, 02.2010, p. 1-7.

Research output: Contribution to journalArticle

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