Intramedullary cavity as an implant site for bioartificial pancreas: An in vivo study on diabetic canine

Kai Chiang Yang, Chang Chin Wu, Shoichiro Sumi, Tzong Fu Kuo, Sheng Chuan Lin, Feng Huei Lin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background. Fibrous tissue outgrowth and hypoxia are the major restrictions for the application of bioartificial pancreas (BAP). Accordingly, the intramedullary cavity is proposed as an implant site, and a BAP constructed of calcium phosphate cement chamber was implanted. Methods. Mouse insulinoma cells were encapsulated in agarose gel and then enclosed in a calcium phosphate cement chamber to fabricate a BAP. BAPs were implanted in the femoral intramedullary cavity of diabetic dogs. Pre-and postprandial blood glucose levels were monitored perioperatively. Blood samples were collected for the analysis of C-peptide level, and physiological conditions were observed at predetermined intervals. BAPs were retrieved at 12 weeks postoperatively for histologic examination. Results. Preprandial blood glucose level of diabetic dogs decreased from 420±25 to 223±47 mg/dL at 1 day postoperatively and was maintained in the range of 259±36 mg/dL for 12 weeks. As serum C-peptide level increased from 5.3±2.8 to 105.7±19.4 pmol/L, the rate of decrease of postprandial blood glucose was accelerated. Histologic examination revealed that recipient bone tissues were binding to the surfaces of BAPs directly; there was no development of fibrous tissue. Immunohistochemical stain was positive for insulin in the enclosed insulinoma cells. CONCLUSIONS.: This study demonstrated that BAPs implanted into the intramedullary cavity functioned well during the experimental period. Thus, the intramedullary cavity can serve as an implant site for BAPs.

Original languageEnglish
Pages (from-to)604-611
Number of pages8
JournalTransplantation
Volume90
Issue number6
DOIs
Publication statusPublished - Sep 27 2010
Externally publishedYes

Fingerprint

Blood Glucose
Canidae
Pancreas
Insulinoma
C-Peptide
Dogs
Thigh
Sepharose
Coloring Agents
Gels
Insulin
Bone and Bones
Serum
calcium phosphate
Hypoxia

Keywords

  • Bioartificial pancreas
  • Calcium phosphate cement
  • Immunoisolation
  • Xenotransplantation

ASJC Scopus subject areas

  • Transplantation
  • Medicine(all)

Cite this

Intramedullary cavity as an implant site for bioartificial pancreas : An in vivo study on diabetic canine. / Yang, Kai Chiang; Wu, Chang Chin; Sumi, Shoichiro; Kuo, Tzong Fu; Lin, Sheng Chuan; Lin, Feng Huei.

In: Transplantation, Vol. 90, No. 6, 27.09.2010, p. 604-611.

Research output: Contribution to journalArticle

Yang, Kai Chiang ; Wu, Chang Chin ; Sumi, Shoichiro ; Kuo, Tzong Fu ; Lin, Sheng Chuan ; Lin, Feng Huei. / Intramedullary cavity as an implant site for bioartificial pancreas : An in vivo study on diabetic canine. In: Transplantation. 2010 ; Vol. 90, No. 6. pp. 604-611.
@article{c540cb6154df4332aec9f76a3f1c0864,
title = "Intramedullary cavity as an implant site for bioartificial pancreas: An in vivo study on diabetic canine",
abstract = "Background. Fibrous tissue outgrowth and hypoxia are the major restrictions for the application of bioartificial pancreas (BAP). Accordingly, the intramedullary cavity is proposed as an implant site, and a BAP constructed of calcium phosphate cement chamber was implanted. Methods. Mouse insulinoma cells were encapsulated in agarose gel and then enclosed in a calcium phosphate cement chamber to fabricate a BAP. BAPs were implanted in the femoral intramedullary cavity of diabetic dogs. Pre-and postprandial blood glucose levels were monitored perioperatively. Blood samples were collected for the analysis of C-peptide level, and physiological conditions were observed at predetermined intervals. BAPs were retrieved at 12 weeks postoperatively for histologic examination. Results. Preprandial blood glucose level of diabetic dogs decreased from 420±25 to 223±47 mg/dL at 1 day postoperatively and was maintained in the range of 259±36 mg/dL for 12 weeks. As serum C-peptide level increased from 5.3±2.8 to 105.7±19.4 pmol/L, the rate of decrease of postprandial blood glucose was accelerated. Histologic examination revealed that recipient bone tissues were binding to the surfaces of BAPs directly; there was no development of fibrous tissue. Immunohistochemical stain was positive for insulin in the enclosed insulinoma cells. CONCLUSIONS.: This study demonstrated that BAPs implanted into the intramedullary cavity functioned well during the experimental period. Thus, the intramedullary cavity can serve as an implant site for BAPs.",
keywords = "Bioartificial pancreas, Calcium phosphate cement, Immunoisolation, Xenotransplantation",
author = "Yang, {Kai Chiang} and Wu, {Chang Chin} and Shoichiro Sumi and Kuo, {Tzong Fu} and Lin, {Sheng Chuan} and Lin, {Feng Huei}",
year = "2010",
month = "9",
day = "27",
doi = "10.1097/TP.0b013e3181ca64d1",
language = "English",
volume = "90",
pages = "604--611",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Intramedullary cavity as an implant site for bioartificial pancreas

T2 - An in vivo study on diabetic canine

AU - Yang, Kai Chiang

AU - Wu, Chang Chin

AU - Sumi, Shoichiro

AU - Kuo, Tzong Fu

AU - Lin, Sheng Chuan

AU - Lin, Feng Huei

PY - 2010/9/27

Y1 - 2010/9/27

N2 - Background. Fibrous tissue outgrowth and hypoxia are the major restrictions for the application of bioartificial pancreas (BAP). Accordingly, the intramedullary cavity is proposed as an implant site, and a BAP constructed of calcium phosphate cement chamber was implanted. Methods. Mouse insulinoma cells were encapsulated in agarose gel and then enclosed in a calcium phosphate cement chamber to fabricate a BAP. BAPs were implanted in the femoral intramedullary cavity of diabetic dogs. Pre-and postprandial blood glucose levels were monitored perioperatively. Blood samples were collected for the analysis of C-peptide level, and physiological conditions were observed at predetermined intervals. BAPs were retrieved at 12 weeks postoperatively for histologic examination. Results. Preprandial blood glucose level of diabetic dogs decreased from 420±25 to 223±47 mg/dL at 1 day postoperatively and was maintained in the range of 259±36 mg/dL for 12 weeks. As serum C-peptide level increased from 5.3±2.8 to 105.7±19.4 pmol/L, the rate of decrease of postprandial blood glucose was accelerated. Histologic examination revealed that recipient bone tissues were binding to the surfaces of BAPs directly; there was no development of fibrous tissue. Immunohistochemical stain was positive for insulin in the enclosed insulinoma cells. CONCLUSIONS.: This study demonstrated that BAPs implanted into the intramedullary cavity functioned well during the experimental period. Thus, the intramedullary cavity can serve as an implant site for BAPs.

AB - Background. Fibrous tissue outgrowth and hypoxia are the major restrictions for the application of bioartificial pancreas (BAP). Accordingly, the intramedullary cavity is proposed as an implant site, and a BAP constructed of calcium phosphate cement chamber was implanted. Methods. Mouse insulinoma cells were encapsulated in agarose gel and then enclosed in a calcium phosphate cement chamber to fabricate a BAP. BAPs were implanted in the femoral intramedullary cavity of diabetic dogs. Pre-and postprandial blood glucose levels were monitored perioperatively. Blood samples were collected for the analysis of C-peptide level, and physiological conditions were observed at predetermined intervals. BAPs were retrieved at 12 weeks postoperatively for histologic examination. Results. Preprandial blood glucose level of diabetic dogs decreased from 420±25 to 223±47 mg/dL at 1 day postoperatively and was maintained in the range of 259±36 mg/dL for 12 weeks. As serum C-peptide level increased from 5.3±2.8 to 105.7±19.4 pmol/L, the rate of decrease of postprandial blood glucose was accelerated. Histologic examination revealed that recipient bone tissues were binding to the surfaces of BAPs directly; there was no development of fibrous tissue. Immunohistochemical stain was positive for insulin in the enclosed insulinoma cells. CONCLUSIONS.: This study demonstrated that BAPs implanted into the intramedullary cavity functioned well during the experimental period. Thus, the intramedullary cavity can serve as an implant site for BAPs.

KW - Bioartificial pancreas

KW - Calcium phosphate cement

KW - Immunoisolation

KW - Xenotransplantation

UR - http://www.scopus.com/inward/record.url?scp=77957267597&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957267597&partnerID=8YFLogxK

U2 - 10.1097/TP.0b013e3181ca64d1

DO - 10.1097/TP.0b013e3181ca64d1

M3 - Article

C2 - 20048693

AN - SCOPUS:77957267597

VL - 90

SP - 604

EP - 611

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 6

ER -