Intracellular acidification enhances neutrophil phagocytosis in chronic haemodialysis patients

Possible role of CD11b/CD18

Chien Sheng Lin, Joseph G. Wann, Cheng Wen Hsiao, Deborah W. Tai, Jin Shuen Chen, Yung Ho Hsu, Huei Chen Huang, Chung Faye Chao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background. We have demonstrated that uraemic neutrophils that exhibit a low intracellular pH (pHi) display enhanced phagocytosis. However, the underlying cellular mechanism is unclear. Methods. We used neutrophils from three groups of haemodialysis (HD) patients before dialysis (Groups A, B and C) and also from age- and sex-matched healthy individuals to determine pHi, phagocytosis and expression of CD11b, CD18, CD14 and toll-like receptors (TLR)-2 and TLR-4. The patients were categorized based on three consecutive monthly pre-dialysis plasma bicarbonate concentrations(PHCO3) and pH values; Groups A, B and C had a constant pre-dialysis PHCO3 of ≤21, 21-26 and ≥26 mmol/L (mEq/L), respectively. We also studied the effects induced by the correction of metabolic acidosis and monoclonal antibodies (mAbs) against CD11b/CD18 on neutrophils in Group A. Furthermore, we investigated the effect of intracellular acidification on uraemic neutrophils ex vivo. Results. We observed that the neutrophils in Group A exhibited significantly increased phagocytosis and expression of CD11b/CD18 compared with those in Groups B and C. Additionally, our ex vivo studies demonstrated that the mAbs against CD11b/CD18 partially blocked the enhancement of neutrophil phagocytosis in Group A. Moreover, the pHi of uraemic neutrophils is inversely correlated with phagocytosis and expression of CD11b/CD18. Conclusions. HD patients with a low PHCO3 exhibited low neutrophil pHi that in turn increased the expression of CD11b/CD18 compared with neutrophils with a normal or high pHi. This increased expression of CD11b/CD18 on the uraemic neutrophils may contribute to the pHi-mediated phagocytosis.

Original languageEnglish
Pages (from-to)1642-1649
Number of pages8
JournalNephrology Dialysis Transplantation
Volume23
Issue number5
DOIs
Publication statusPublished - May 2008

Fingerprint

Phagocytosis
Renal Dialysis
Neutrophils
Dialysis
Monoclonal Antibodies
Toll-Like Receptor 2
Toll-Like Receptor 4
Bicarbonates
Acidosis

Keywords

  • CD11b/CD18
  • Haemodialysis
  • Intracellular pH
  • Neutrophil
  • Phagocytosis

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Intracellular acidification enhances neutrophil phagocytosis in chronic haemodialysis patients : Possible role of CD11b/CD18. / Lin, Chien Sheng; Wann, Joseph G.; Hsiao, Cheng Wen; Tai, Deborah W.; Chen, Jin Shuen; Hsu, Yung Ho; Huang, Huei Chen; Chao, Chung Faye.

In: Nephrology Dialysis Transplantation, Vol. 23, No. 5, 05.2008, p. 1642-1649.

Research output: Contribution to journalArticle

Lin, Chien Sheng ; Wann, Joseph G. ; Hsiao, Cheng Wen ; Tai, Deborah W. ; Chen, Jin Shuen ; Hsu, Yung Ho ; Huang, Huei Chen ; Chao, Chung Faye. / Intracellular acidification enhances neutrophil phagocytosis in chronic haemodialysis patients : Possible role of CD11b/CD18. In: Nephrology Dialysis Transplantation. 2008 ; Vol. 23, No. 5. pp. 1642-1649.
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abstract = "Background. We have demonstrated that uraemic neutrophils that exhibit a low intracellular pH (pHi) display enhanced phagocytosis. However, the underlying cellular mechanism is unclear. Methods. We used neutrophils from three groups of haemodialysis (HD) patients before dialysis (Groups A, B and C) and also from age- and sex-matched healthy individuals to determine pHi, phagocytosis and expression of CD11b, CD18, CD14 and toll-like receptors (TLR)-2 and TLR-4. The patients were categorized based on three consecutive monthly pre-dialysis plasma bicarbonate concentrations(PHCO3) and pH values; Groups A, B and C had a constant pre-dialysis PHCO3 of ≤21, 21-26 and ≥26 mmol/L (mEq/L), respectively. We also studied the effects induced by the correction of metabolic acidosis and monoclonal antibodies (mAbs) against CD11b/CD18 on neutrophils in Group A. Furthermore, we investigated the effect of intracellular acidification on uraemic neutrophils ex vivo. Results. We observed that the neutrophils in Group A exhibited significantly increased phagocytosis and expression of CD11b/CD18 compared with those in Groups B and C. Additionally, our ex vivo studies demonstrated that the mAbs against CD11b/CD18 partially blocked the enhancement of neutrophil phagocytosis in Group A. Moreover, the pHi of uraemic neutrophils is inversely correlated with phagocytosis and expression of CD11b/CD18. Conclusions. HD patients with a low PHCO3 exhibited low neutrophil pHi that in turn increased the expression of CD11b/CD18 compared with neutrophils with a normal or high pHi. This increased expression of CD11b/CD18 on the uraemic neutrophils may contribute to the pHi-mediated phagocytosis.",
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T1 - Intracellular acidification enhances neutrophil phagocytosis in chronic haemodialysis patients

T2 - Possible role of CD11b/CD18

AU - Lin, Chien Sheng

AU - Wann, Joseph G.

AU - Hsiao, Cheng Wen

AU - Tai, Deborah W.

AU - Chen, Jin Shuen

AU - Hsu, Yung Ho

AU - Huang, Huei Chen

AU - Chao, Chung Faye

PY - 2008/5

Y1 - 2008/5

N2 - Background. We have demonstrated that uraemic neutrophils that exhibit a low intracellular pH (pHi) display enhanced phagocytosis. However, the underlying cellular mechanism is unclear. Methods. We used neutrophils from three groups of haemodialysis (HD) patients before dialysis (Groups A, B and C) and also from age- and sex-matched healthy individuals to determine pHi, phagocytosis and expression of CD11b, CD18, CD14 and toll-like receptors (TLR)-2 and TLR-4. The patients were categorized based on three consecutive monthly pre-dialysis plasma bicarbonate concentrations(PHCO3) and pH values; Groups A, B and C had a constant pre-dialysis PHCO3 of ≤21, 21-26 and ≥26 mmol/L (mEq/L), respectively. We also studied the effects induced by the correction of metabolic acidosis and monoclonal antibodies (mAbs) against CD11b/CD18 on neutrophils in Group A. Furthermore, we investigated the effect of intracellular acidification on uraemic neutrophils ex vivo. Results. We observed that the neutrophils in Group A exhibited significantly increased phagocytosis and expression of CD11b/CD18 compared with those in Groups B and C. Additionally, our ex vivo studies demonstrated that the mAbs against CD11b/CD18 partially blocked the enhancement of neutrophil phagocytosis in Group A. Moreover, the pHi of uraemic neutrophils is inversely correlated with phagocytosis and expression of CD11b/CD18. Conclusions. HD patients with a low PHCO3 exhibited low neutrophil pHi that in turn increased the expression of CD11b/CD18 compared with neutrophils with a normal or high pHi. This increased expression of CD11b/CD18 on the uraemic neutrophils may contribute to the pHi-mediated phagocytosis.

AB - Background. We have demonstrated that uraemic neutrophils that exhibit a low intracellular pH (pHi) display enhanced phagocytosis. However, the underlying cellular mechanism is unclear. Methods. We used neutrophils from three groups of haemodialysis (HD) patients before dialysis (Groups A, B and C) and also from age- and sex-matched healthy individuals to determine pHi, phagocytosis and expression of CD11b, CD18, CD14 and toll-like receptors (TLR)-2 and TLR-4. The patients were categorized based on three consecutive monthly pre-dialysis plasma bicarbonate concentrations(PHCO3) and pH values; Groups A, B and C had a constant pre-dialysis PHCO3 of ≤21, 21-26 and ≥26 mmol/L (mEq/L), respectively. We also studied the effects induced by the correction of metabolic acidosis and monoclonal antibodies (mAbs) against CD11b/CD18 on neutrophils in Group A. Furthermore, we investigated the effect of intracellular acidification on uraemic neutrophils ex vivo. Results. We observed that the neutrophils in Group A exhibited significantly increased phagocytosis and expression of CD11b/CD18 compared with those in Groups B and C. Additionally, our ex vivo studies demonstrated that the mAbs against CD11b/CD18 partially blocked the enhancement of neutrophil phagocytosis in Group A. Moreover, the pHi of uraemic neutrophils is inversely correlated with phagocytosis and expression of CD11b/CD18. Conclusions. HD patients with a low PHCO3 exhibited low neutrophil pHi that in turn increased the expression of CD11b/CD18 compared with neutrophils with a normal or high pHi. This increased expression of CD11b/CD18 on the uraemic neutrophils may contribute to the pHi-mediated phagocytosis.

KW - CD11b/CD18

KW - Haemodialysis

KW - Intracellular pH

KW - Neutrophil

KW - Phagocytosis

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DO - 10.1093/ndt/gfm852

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VL - 23

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JO - Nephrology Dialysis Transplantation

JF - Nephrology Dialysis Transplantation

SN - 0931-0509

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