Intra-articular injection of collagenase induced experimental osteoarthritis of the lumbar facet joint in rats

Tsu Te Yeh, Zhi Hong Wen, Herng Sheng Lee, Chian Her Lee, Zhi Yang, Yen Hsuan Jean, Shing-Sheng Wu, Marcel E. Nimni, Bo Han

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We aimed to establish an animal model to investigate primary osteoarthritis of the lumbar facet joints after collagenase injection in rats and its effects on chondrocyte apoptosis. We hypothesized that osteoarthritic-like changes would be induced by collagenase injection and that apoptosis of chondrocytes would increase. Collagenase (1, 10, or 50 U) or saline (control) was injected into the lumbar facet joints. The histology and histochemistry of cartilage, synovium, and subchondral bone were examined at 1, 3, and 6 weeks after surgery. Apoptotic cells induced by 1 U of collagenase were quantified using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay. Degeneration of the cartilage and changes to the synovium and subchondral bone were dependent on both the doses of collagenase and the time after surgery. There were significantly more apoptotic chondrocytes in collagenase-treated joints than in control (P <0.001 at 1 and 3 weeks and P <0.05 at 6 weeks). Thus, lumbar facet joints subjected to collagenase developed osteoarthritic-like changes that could be quantified and compared. This model provides a useful tool for further study on the effects of compounds that have the potential to inhibit enzyme-associated damage to cartilage.

Original languageEnglish
Pages (from-to)734-742
Number of pages9
JournalEuropean Spine Journal
Volume17
Issue number5
DOIs
Publication statusPublished - May 2008

Fingerprint

Spine Osteoarthritis
Zygapophyseal Joint
Intra-Articular Injections
Collagenases
Chondrocytes
Cartilage
Synovial Membrane
Apoptosis
Bone and Bones
Injections
DNA Nucleotidylexotransferase
Histology
Animal Models
Joints
Enzymes

Keywords

  • Apoptosis
  • Collagenase
  • Facet joint
  • Osteoarthritis
  • Zygapophysial joint

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Intra-articular injection of collagenase induced experimental osteoarthritis of the lumbar facet joint in rats. / Yeh, Tsu Te; Wen, Zhi Hong; Lee, Herng Sheng; Lee, Chian Her; Yang, Zhi; Jean, Yen Hsuan; Wu, Shing-Sheng; Nimni, Marcel E.; Han, Bo.

In: European Spine Journal, Vol. 17, No. 5, 05.2008, p. 734-742.

Research output: Contribution to journalArticle

Yeh, Tsu Te ; Wen, Zhi Hong ; Lee, Herng Sheng ; Lee, Chian Her ; Yang, Zhi ; Jean, Yen Hsuan ; Wu, Shing-Sheng ; Nimni, Marcel E. ; Han, Bo. / Intra-articular injection of collagenase induced experimental osteoarthritis of the lumbar facet joint in rats. In: European Spine Journal. 2008 ; Vol. 17, No. 5. pp. 734-742.
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