Interplay of posttranslational modifications in Sp1 mediates Sp1 stability during cell cycle progression

Yi Ting Wang, Wen Bin Yang, Wen Chang Chang, Jan Jong Hung

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Although Sp1 is known to undergo posttranslational modifications such as phosphorylation, glycosylation, acetylation, sumoylation, and ubiquitination, little is known about the possible interplay between the different forms of Sp1 that may affect its overall levels. It is also unknown whether changes in the levels of Sp1 influence any biological cell processes. Here, we identified RNF4 as the ubiquitin E3 ligase of Sp1. From in vitro and in vivo experiments, we found that sumoylated Sp1 can recruit RNF4 as a ubiquitin E3 ligase that subjects sumoylated Sp1 to proteasomal degradation. Sp1 mapping revealed two ubiquitination-related domains: a small ubiquitin-like modifier in the N-terminus of Sp1(Lys16) and the C-terminus of Sp1 that directly interacts with RNF4. Interestingly, when Sp1 was phosphorylated at Thr739 by c-Jun NH 2-terminal kinase 1 during mitosis, this phosphorylated form of Sp1 abolished the Sp1-RNF4 interaction. Our results show that, while sumoylated Sp1 subjects to proteasomal degradation, the phosphorylation that occurs during the cell cycle can protect Sp1 from degradation by repressing the Sp1-RNF4 interaction. Thus, we propose that the interplay between posttranslational modifications of Sp1 plays an important role in cell cycle progression and keeps Sp1 at a critical level for mitosis.

Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalJournal of Molecular Biology
Volume414
Issue number1
DOIs
Publication statusPublished - Nov 18 2011

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Ubiquitin-Protein Ligases
Ubiquitination
Post Translational Protein Processing
Mitosis
Cell Cycle
Phosphorylation
Sumoylation
Biological Phenomena
Acetylation
Ubiquitin
Glycosylation
Phosphotransferases
In Vitro Techniques

Keywords

  • phosphorylation
  • RNF4
  • sumoylation
  • ubiquitin E3 ligase

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Interplay of posttranslational modifications in Sp1 mediates Sp1 stability during cell cycle progression. / Wang, Yi Ting; Yang, Wen Bin; Chang, Wen Chang; Hung, Jan Jong.

In: Journal of Molecular Biology, Vol. 414, No. 1, 18.11.2011, p. 1-14.

Research output: Contribution to journalArticle

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