Internalization of mesoporous silica nanoparticles induces transient but not sufficient osteogenic signals in human mesenchymal stem cells

Dong Ming Huang, Tsai Hua Chung, Yann Hung, Fang Lu, Si Han Wu, Chung Yuan Mou, Ming Yao, Yao Chang Chen

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

The biocompatibility of nanoparticles is the prerequisite for their applications in biomedicine but can be misleading due to the absence of criteria for evaluating the safety and toxicity of those nanomaterials. Recent studies indicate that mesoporous silica nanoparticles (MSNs) can easily internalize into human mesenchymal stem cells (hMSCs) without apparent deleterious effects on cellular growth or differentiation, and hence are emerging as an ideal stem cell labeling agent. The objective of this study was to thoroughly investigate the effect of MSNs on osteogenesis induction and to examine their biocompatibility in hMSCs. Uptake of MSNs into hMSCs did not affect the cell viability, proliferation and regular osteogenic differentiation of the cells. However, the internalization of MSNs indeed induced actin polymerization and activated the small GTP-bound protein RhoA. The MSN-induced cellular protein responses as believed to cause osteogenesis of hMSCs did not result in promotion of regular osteogenic differentiation as analyzed by cytochemical stain and protein activity assay of alkaline phosphatase (ALP). When the effect of MSNs on ALP gene expression was further examined by reverse transcriptase polymerase chain reaction, MSN-treated hMSCs were shown to have significantly higher mRNA expression than control cells after 1-hour osteogenic induction. The induction of ALP gene expression by MSNs, however, was absent in cells after 1-day incubation with osteogenic differentiation. Together our results show that the internalization of MSNs had a significant effect on the transient protein response and osteogenic signal in hMSCs, thereby suggesting that the effects of nanoparticles on diverse aspects of cellular activities should be carefully evaluated even though the nanoparticles are generally considered as biocompatible at present.

Original languageEnglish
Pages (from-to)208-215
Number of pages8
JournalToxicology and Applied Pharmacology
Volume231
Issue number2
DOIs
Publication statusPublished - Sep 1 2008
Externally publishedYes

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Silicon Dioxide
Nanoparticles
Alkaline Phosphatase
Biocompatibility
Osteogenesis
Gene expression
rhoA GTP-Binding Protein
Gene Expression
Proteins
Nanostructures
Polymerase chain reaction
RNA-Directed DNA Polymerase
Cell proliferation
Guanosine Triphosphate
Reverse Transcriptase Polymerase Chain Reaction
Nanostructured materials
Polymerization
Labeling

Keywords

  • Actin cytoskeleton
  • Cytotoxicity
  • Mesenchymal stem cells
  • Nanoparticles
  • Osteogenic differentiation
  • RhoA

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Internalization of mesoporous silica nanoparticles induces transient but not sufficient osteogenic signals in human mesenchymal stem cells. / Huang, Dong Ming; Chung, Tsai Hua; Hung, Yann; Lu, Fang; Wu, Si Han; Mou, Chung Yuan; Yao, Ming; Chen, Yao Chang.

In: Toxicology and Applied Pharmacology, Vol. 231, No. 2, 01.09.2008, p. 208-215.

Research output: Contribution to journalArticle

Huang, Dong Ming ; Chung, Tsai Hua ; Hung, Yann ; Lu, Fang ; Wu, Si Han ; Mou, Chung Yuan ; Yao, Ming ; Chen, Yao Chang. / Internalization of mesoporous silica nanoparticles induces transient but not sufficient osteogenic signals in human mesenchymal stem cells. In: Toxicology and Applied Pharmacology. 2008 ; Vol. 231, No. 2. pp. 208-215.
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