Interleukin (IL)-19 promoted skin wound healing by increasing fibroblast keratinocyte growth factor expression

Ding Ping Sun, Ching Hua Yeh, Edmund So, Li Yun Wang, Tsui Shan Wei, Ming Shi Chang, Chung-Hsi Hsing

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Interleukin (IL)-19, a member of the IL-10 cytokine family, is involved in keratinocyte proliferation in psoriasis. Objectives: We investigated the role of IL-19 in the wound-healing process in vivo and in vitro. Methods: Two full-thickness circular wounds (4. mm in diameter) were punched into the skin of BALB/C mice. IL-19 and keratinocyte growth factor (KGF) mRNA in wounded skin were determined using real-time PCR. The wounds were treated with PBS, vehicle, IL-19 (400. ng/mL), or IL-20 (400. ng/mL) (. n=. 6 in each group) twice daily and the percentage of wound healing was measured daily for 7. days. In vitro, human skin fibroblast CCD966-SK cells and keratinocyte HaCaT cells were treated with IL-19 or KGF. Cell proliferation and migration were determined using bromodeoxyuridine (BrdU) and transwell assays, respectively. The expression of IL-19 and KGF mRNA was also analyzed. Results: In wounded mouse skin, IL-19 mRNA was upregulated at 12. h, and KGF at 24. h after the injury. Both increases in gene expression declined 72. h after the skin had been wounded. The percentage of wound healing in IL-19-treated mice was higher than in control mice. In vitro, IL-19 upregulated KGF expression in the CCD966-SK cells; IL-19 was upregulated in KGF-treated HaCaT cells. KGF but not IL-19 promoted HaCaT cell proliferation. However, IL-19 significantly increased the migration of HaCaT cells. HaCaT cells treated with the cultured supernatants of IL-19-stimulated CCD966-SK cells showed significantly more proliferation than in controls. Conclusions: IL-19 is important for cutaneous wound healing because it upregulates KGF expression.

Original languageEnglish
Pages (from-to)360-368
Number of pages9
JournalCytokine
Volume62
Issue number3
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Fibroblast Growth Factor 7
Fibroblast Growth Factors
Interleukins
Fibroblasts
Wound Healing
Skin
Cell proliferation
Keratinocytes
Messenger RNA
Cell Movement
Wounds and Injuries
Inbred BALB C Mouse
Cell Proliferation
Bromodeoxyuridine
Psoriasis

Keywords

  • Cytokines
  • Interleukin-19
  • Keratinocyte growth factor
  • Wound healing

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

Sun, D. P., Yeh, C. H., So, E., Wang, L. Y., Wei, T. S., Chang, M. S., & Hsing, C-H. (2013). Interleukin (IL)-19 promoted skin wound healing by increasing fibroblast keratinocyte growth factor expression. Cytokine, 62(3), 360-368. https://doi.org/10.1016/j.cyto.2013.03.017

Interleukin (IL)-19 promoted skin wound healing by increasing fibroblast keratinocyte growth factor expression. / Sun, Ding Ping; Yeh, Ching Hua; So, Edmund; Wang, Li Yun; Wei, Tsui Shan; Chang, Ming Shi; Hsing, Chung-Hsi.

In: Cytokine, Vol. 62, No. 3, 06.2013, p. 360-368.

Research output: Contribution to journalArticle

Sun, DP, Yeh, CH, So, E, Wang, LY, Wei, TS, Chang, MS & Hsing, C-H 2013, 'Interleukin (IL)-19 promoted skin wound healing by increasing fibroblast keratinocyte growth factor expression', Cytokine, vol. 62, no. 3, pp. 360-368. https://doi.org/10.1016/j.cyto.2013.03.017
Sun, Ding Ping ; Yeh, Ching Hua ; So, Edmund ; Wang, Li Yun ; Wei, Tsui Shan ; Chang, Ming Shi ; Hsing, Chung-Hsi. / Interleukin (IL)-19 promoted skin wound healing by increasing fibroblast keratinocyte growth factor expression. In: Cytokine. 2013 ; Vol. 62, No. 3. pp. 360-368.
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abstract = "Background: Interleukin (IL)-19, a member of the IL-10 cytokine family, is involved in keratinocyte proliferation in psoriasis. Objectives: We investigated the role of IL-19 in the wound-healing process in vivo and in vitro. Methods: Two full-thickness circular wounds (4. mm in diameter) were punched into the skin of BALB/C mice. IL-19 and keratinocyte growth factor (KGF) mRNA in wounded skin were determined using real-time PCR. The wounds were treated with PBS, vehicle, IL-19 (400. ng/mL), or IL-20 (400. ng/mL) (. n=. 6 in each group) twice daily and the percentage of wound healing was measured daily for 7. days. In vitro, human skin fibroblast CCD966-SK cells and keratinocyte HaCaT cells were treated with IL-19 or KGF. Cell proliferation and migration were determined using bromodeoxyuridine (BrdU) and transwell assays, respectively. The expression of IL-19 and KGF mRNA was also analyzed. Results: In wounded mouse skin, IL-19 mRNA was upregulated at 12. h, and KGF at 24. h after the injury. Both increases in gene expression declined 72. h after the skin had been wounded. The percentage of wound healing in IL-19-treated mice was higher than in control mice. In vitro, IL-19 upregulated KGF expression in the CCD966-SK cells; IL-19 was upregulated in KGF-treated HaCaT cells. KGF but not IL-19 promoted HaCaT cell proliferation. However, IL-19 significantly increased the migration of HaCaT cells. HaCaT cells treated with the cultured supernatants of IL-19-stimulated CCD966-SK cells showed significantly more proliferation than in controls. Conclusions: IL-19 is important for cutaneous wound healing because it upregulates KGF expression.",
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AU - Yeh, Ching Hua

AU - So, Edmund

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AU - Wei, Tsui Shan

AU - Chang, Ming Shi

AU - Hsing, Chung-Hsi

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AB - Background: Interleukin (IL)-19, a member of the IL-10 cytokine family, is involved in keratinocyte proliferation in psoriasis. Objectives: We investigated the role of IL-19 in the wound-healing process in vivo and in vitro. Methods: Two full-thickness circular wounds (4. mm in diameter) were punched into the skin of BALB/C mice. IL-19 and keratinocyte growth factor (KGF) mRNA in wounded skin were determined using real-time PCR. The wounds were treated with PBS, vehicle, IL-19 (400. ng/mL), or IL-20 (400. ng/mL) (. n=. 6 in each group) twice daily and the percentage of wound healing was measured daily for 7. days. In vitro, human skin fibroblast CCD966-SK cells and keratinocyte HaCaT cells were treated with IL-19 or KGF. Cell proliferation and migration were determined using bromodeoxyuridine (BrdU) and transwell assays, respectively. The expression of IL-19 and KGF mRNA was also analyzed. Results: In wounded mouse skin, IL-19 mRNA was upregulated at 12. h, and KGF at 24. h after the injury. Both increases in gene expression declined 72. h after the skin had been wounded. The percentage of wound healing in IL-19-treated mice was higher than in control mice. In vitro, IL-19 upregulated KGF expression in the CCD966-SK cells; IL-19 was upregulated in KGF-treated HaCaT cells. KGF but not IL-19 promoted HaCaT cell proliferation. However, IL-19 significantly increased the migration of HaCaT cells. HaCaT cells treated with the cultured supernatants of IL-19-stimulated CCD966-SK cells showed significantly more proliferation than in controls. Conclusions: IL-19 is important for cutaneous wound healing because it upregulates KGF expression.

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