Interleukin-5 in growth and differentiation of blood eosinophil progenitors in asthma

Effect of glucocorticoids

Han Pin Kuo, Chun Hua Wang, Horng Chyuan Lin, Kuo Shiung Hwang, Shu Liang Liu, Kian Fan Chung

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

There are increased numbers of circulating CD34+ progenitor cells for eosinophils in patients with atopic asthma, with a further increase following allergen exposure or spontaneous worsening of asthma. We investigated the expression of IL-5 and IL-5Rα receptor in circulating CD34+ progenitor cells in allergic asthmatics and the effects of corticosteroids. Using double-staining techniques, up to 50% of CD34+ cells expressed intracellular IL-5, and by RT-PCR, there was significant expression of IL-5 mRNA. When cultured in a semi-liquid methylcellulose medium, there were more eosinophil colony-forming units grown from asthmatic non-adherent mononuclear cell depleted of T cells in the presence of the growth factors GM-CSF, SCF and IL-3, but not of IL-5. An anti-IL-5Rcα receptor antibody and an anti-sense IL-5 oligonucleotide reduced the number of eosinophil colony forming units. No IL-5 mRNA or protein expression on T cells was observed in asthmatics or normal subjects. In the presence of growth factors including IL-5, there were significantly greater colony numbers with eosinophilic lineage grown from either asthmatics or normal subjects. Dexamethasone (10-6 M) suppressed IL-5 mRNA and protein expression in CD34+ cells, and reduced eosinophil colony-forming units in asthmatics, but not in normal subjects. Dexamethasone did not change the expression of IL-5Rα on CD34+ cells. We conclude that there is increased expression of IL-5 on blood CD34+ cells of patients with asthma and that this expression may auto-regulate eosinophilic colony formation from these progenitor cells. Corticosteroids inhibit the expression of IL-5 in circulating CD34+ progenitor cells.

Original languageEnglish
Pages (from-to)1539-1547
Number of pages9
JournalBritish Journal of Pharmacology
Volume134
Issue number7
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

Fingerprint

Interleukin-5
Eosinophils
Glucocorticoids
Asthma
Stem Cells
Growth
Messenger RNA
Dexamethasone
Intercellular Signaling Peptides and Proteins
Adrenal Cortex Hormones
T-Lymphocytes
Methylcellulose
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Oligonucleotides
Allergens
Anti-Idiotypic Antibodies
Blood Cells
Proteins
Staining and Labeling

Keywords

  • Asthma
  • Corticosteroids
  • Eosinophil
  • Eosinophil progenitors
  • Interleukin-5
  • Interleukin-5 receptor

ASJC Scopus subject areas

  • Pharmacology

Cite this

Interleukin-5 in growth and differentiation of blood eosinophil progenitors in asthma : Effect of glucocorticoids. / Kuo, Han Pin; Wang, Chun Hua; Lin, Horng Chyuan; Hwang, Kuo Shiung; Liu, Shu Liang; Chung, Kian Fan.

In: British Journal of Pharmacology, Vol. 134, No. 7, 01.01.2001, p. 1539-1547.

Research output: Contribution to journalArticle

Kuo, Han Pin ; Wang, Chun Hua ; Lin, Horng Chyuan ; Hwang, Kuo Shiung ; Liu, Shu Liang ; Chung, Kian Fan. / Interleukin-5 in growth and differentiation of blood eosinophil progenitors in asthma : Effect of glucocorticoids. In: British Journal of Pharmacology. 2001 ; Vol. 134, No. 7. pp. 1539-1547.
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