Interleukin-5 in growth and differentiation of blood eosinophil progenitors in asthma: Effect of glucocorticoids

Han Pin Kuo, Chun Hua Wang, Horng Chyuan Lin, Kuo Shiung Hwang, Shu Liang Liu, Kian Fan Chung

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

There are increased numbers of circulating CD34+ progenitor cells for eosinophils in patients with atopic asthma, with a further increase following allergen exposure or spontaneous worsening of asthma. We investigated the expression of IL-5 and IL-5Rα receptor in circulating CD34+ progenitor cells in allergic asthmatics and the effects of corticosteroids. Using double-staining techniques, up to 50% of CD34+ cells expressed intracellular IL-5, and by RT-PCR, there was significant expression of IL-5 mRNA. When cultured in a semi-liquid methylcellulose medium, there were more eosinophil colony-forming units grown from asthmatic non-adherent mononuclear cell depleted of T cells in the presence of the growth factors GM-CSF, SCF and IL-3, but not of IL-5. An anti-IL-5Rcα receptor antibody and an anti-sense IL-5 oligonucleotide reduced the number of eosinophil colony forming units. No IL-5 mRNA or protein expression on T cells was observed in asthmatics or normal subjects. In the presence of growth factors including IL-5, there were significantly greater colony numbers with eosinophilic lineage grown from either asthmatics or normal subjects. Dexamethasone (10-6 M) suppressed IL-5 mRNA and protein expression in CD34+ cells, and reduced eosinophil colony-forming units in asthmatics, but not in normal subjects. Dexamethasone did not change the expression of IL-5Rα on CD34+ cells. We conclude that there is increased expression of IL-5 on blood CD34+ cells of patients with asthma and that this expression may auto-regulate eosinophilic colony formation from these progenitor cells. Corticosteroids inhibit the expression of IL-5 in circulating CD34+ progenitor cells.

Original languageEnglish
Pages (from-to)1539-1547
Number of pages9
JournalBritish Journal of Pharmacology
Volume134
Issue number7
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

Fingerprint

Interleukin-5
Eosinophils
Glucocorticoids
Asthma
Stem Cells
Growth
Messenger RNA
Dexamethasone
Intercellular Signaling Peptides and Proteins
Adrenal Cortex Hormones
T-Lymphocytes
Methylcellulose
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Oligonucleotides
Allergens
Anti-Idiotypic Antibodies
Blood Cells
Proteins
Staining and Labeling

Keywords

  • Asthma
  • Corticosteroids
  • Eosinophil
  • Eosinophil progenitors
  • Interleukin-5
  • Interleukin-5 receptor

ASJC Scopus subject areas

  • Pharmacology

Cite this

Interleukin-5 in growth and differentiation of blood eosinophil progenitors in asthma : Effect of glucocorticoids. / Kuo, Han Pin; Wang, Chun Hua; Lin, Horng Chyuan; Hwang, Kuo Shiung; Liu, Shu Liang; Chung, Kian Fan.

In: British Journal of Pharmacology, Vol. 134, No. 7, 01.01.2001, p. 1539-1547.

Research output: Contribution to journalArticle

Kuo, Han Pin ; Wang, Chun Hua ; Lin, Horng Chyuan ; Hwang, Kuo Shiung ; Liu, Shu Liang ; Chung, Kian Fan. / Interleukin-5 in growth and differentiation of blood eosinophil progenitors in asthma : Effect of glucocorticoids. In: British Journal of Pharmacology. 2001 ; Vol. 134, No. 7. pp. 1539-1547.
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