Interleukin 15 activates Akt to protect astrocytes from oxygen glucose deprivation-induced cell death

Gilbert Aaron Lee, Yein Gei Lai, Ray Jade Chen, Nan Shih Liao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Astrocytes play a pivotal role in neuronal survival under the condition of post-ischemic brain inflammation, but the relevant astrocyte-derived mediators of ischemic brain injury remain to be defined. IL-15 supports survival of multiple lymphocyte lineages in the peripheral immune system, but the role of IL-15 in inflammatory disease of the central nervous system is not well defined. Recent research has shown an increase of IL-15-expressing astrocytes in the ischemic brain. Since astrocytes promote neuron survival under cerebral ischemia by buffering excess extracellular glutamate and producing growth factors, recovery of astrocyte function could be of benefit for stroke therapy. Here, we report that IL-15 is the pro-survival cytokine that prevents astrocyte death from oxygen glucose deprivation (OGD)-induced damage. Astrocytes up-regulate expression of the IL-15/IL-15Rα complex under OGD, whereas OGD down-regulates the levels of pSTAT5 and pAkt in astrocytes. IL-15 treatment ameliorates the decline of pAkt, decreases the percentage of annexin V+ cells, inhibits the activation of caspase-3, and activates the Akt pathway to promote astrocyte survival in response to OGD. We further identified that activation of Akt, but not PKCα/βI, is essential for astrocyte survival under OGD. Taken together, this study reveals the function of IL-15 in astrocyte survival via Akt phosphorylation in response to OGD-induced damage.

Original languageEnglish
Pages (from-to)68-74
Number of pages7
JournalCytokine
Volume92
DOIs
Publication statusPublished - Apr 1 2017

Fingerprint

Interleukin-15
Cell death
Astrocytes
Cell Death
Oxygen
Glucose
Brain
Chemical activation
Phosphorylation
Lymphocytes
Immune system
Annexin A5
Central Nervous System Diseases
Recovery of Function
Neurology
Encephalitis
Brain Ischemia
Caspase 3
Brain Injuries
Neurons

Keywords

  • Akt
  • Astrocyte
  • IL-15
  • Oxygen glucose deprivation
  • PKC

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Interleukin 15 activates Akt to protect astrocytes from oxygen glucose deprivation-induced cell death. / Lee, Gilbert Aaron; Lai, Yein Gei; Chen, Ray Jade; Liao, Nan Shih.

In: Cytokine, Vol. 92, 01.04.2017, p. 68-74.

Research output: Contribution to journalArticle

Lee, Gilbert Aaron ; Lai, Yein Gei ; Chen, Ray Jade ; Liao, Nan Shih. / Interleukin 15 activates Akt to protect astrocytes from oxygen glucose deprivation-induced cell death. In: Cytokine. 2017 ; Vol. 92. pp. 68-74.
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