Interleukin-10 protects lipopolysaccharide-induced neurotoxicity in primary midbrain cultures by inhibiting the function of NADPH oxidase

Li Qian, Michelle L. Block, Sung Jen Wei, Chiou Feng Lin, Jeffrey Reece, Hao Pang, Belinda Wilson, Jau Shyong Hong, Patrick M. Flood

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Abstract

The role of anti-inflammatory cytokines in Parkinson's disease is not completely understood. In this study, using mesencephalic neuron-glia cultures, we report that both pretreatment and post-treatment of rat mesencephalic neuron-glia cultures with interleukin (IL)-10, a natural immune modulator, reduced lipopolysaccharide (LPS)-induced DA neurotoxicity. The main purpose of this study was to elucidate the molecular mechanism underlying IL-10-elicited neuroprotection. IL-10 significantly inhibited LPS-induced production of tumor necrosis factor-α, nitric oxide, and extracellular superoxide in microglia cells. In addition, using reconstituted neuron and glia cell cultures, IL-10 was shown to be neuroprotective only in the presence of microglia. More importantly, IL-10 failed to protect DA neurons in cultures from mice lacking NADPH oxidase (PHOX), a key enzyme for extracellular superoxide production in immune cells, suggesting the critical role of PHOX in IL-10 neuroprotection. This conclusion was further supported by the finding that IL-10 inhibited LPS-induced translocation of the cytosolic subunit of NADPH oxidase p47 phox to the membrane. When the Janus tyrosine kinase (JAK) 1 signaling pathway was blocked, IL-10 failed to attenuate LPS-induced superoxide production, indicating that the JAK1 signaling cascade mediates the inhibitory effect of IL-10. Together, our results suggest that IL-10 inhibits LPS-induced DA neurotoxicity through the inhibition of PHOX activity in a JAK1-dependent mechanism.

Original languageEnglish
Pages (from-to)44-52
Number of pages9
JournalJournal of Pharmacology and Experimental Therapeutics
Volume319
Issue number1
DOIs
Publication statusPublished - 2006
Externally publishedYes

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NADPH Oxidase
Mesencephalon
Interleukin-10
Lipopolysaccharides
Neuroglia
Superoxides
Neurons
Microglia
Janus Kinase 1
Protein-Tyrosine Kinases
Parkinson Disease
Nitric Oxide
Anti-Inflammatory Agents
Cell Culture Techniques
Tumor Necrosis Factor-alpha
Cytokines
Membranes

ASJC Scopus subject areas

  • Pharmacology

Cite this

Interleukin-10 protects lipopolysaccharide-induced neurotoxicity in primary midbrain cultures by inhibiting the function of NADPH oxidase. / Qian, Li; Block, Michelle L.; Wei, Sung Jen; Lin, Chiou Feng; Reece, Jeffrey; Pang, Hao; Wilson, Belinda; Hong, Jau Shyong; Flood, Patrick M.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 319, No. 1, 2006, p. 44-52.

Research output: Contribution to journalArticle

Qian, Li ; Block, Michelle L. ; Wei, Sung Jen ; Lin, Chiou Feng ; Reece, Jeffrey ; Pang, Hao ; Wilson, Belinda ; Hong, Jau Shyong ; Flood, Patrick M. / Interleukin-10 protects lipopolysaccharide-induced neurotoxicity in primary midbrain cultures by inhibiting the function of NADPH oxidase. In: Journal of Pharmacology and Experimental Therapeutics. 2006 ; Vol. 319, No. 1. pp. 44-52.
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