Interleukin-10 and Th2 cytokines differentially regulate osteopontin expression in human monocytes and dendritic cells

Satoshi Konno, John A. Eckman, Beverly Plunkett, Xinfang Li, Jeffery S. Berman, John Schroeder, Shau Ku Huang

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33 Citations (Scopus)


Osteopontin (OPN) is a pleiotrophic phosphoprotein involved in homeostatic and pathophysiologic responses. It is known to be a chemotactic cytokine for dendritic cells (DCs), a critical cell type in both innate and adaptive immune responses. We report herein a contrasting role of interleukin-10 (IL-10) and Th2 cytokines in the regulation of OPN expression in human monocytes and monocyte-derived DCs (Mo-DCs). Our results showed first that the expression of OPN in monocytes and Mo-DCs was induced in a time-dependent and dose-dependent manner by IL-10 but was inhibited by IL-4 or IL-13. Further, the basal level of OPN expression was also inhibited by IL-4. This inhibitory effect of IL-4 was associated with a faster decay of OPN transcripts and a decreased proximal promoter activity of OPN in IL-4-treated cells. These results demonstrate a novel role of IL-10 and Th2 cytokines in the regulation of DC function through their contrasting regulatory activities on the expression of OPN.

Original languageEnglish
Pages (from-to)562-567
Number of pages6
JournalJournal of Interferon and Cytokine Research
Issue number8
Publication statusPublished - Aug 1 2006
Externally publishedYes


ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Virology

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