Interleukin-10 and Th2 cytokines differentially regulate osteopontin expression in human monocytes and dendritic cells

Satoshi Konno, John A. Eckman, Beverly Plunkett, Xinfang Li, Jeffery S. Berman, John Schroeder, Shau Ku Huang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Osteopontin (OPN) is a pleiotrophic phosphoprotein involved in homeostatic and pathophysiologic responses. It is known to be a chemotactic cytokine for dendritic cells (DCs), a critical cell type in both innate and adaptive immune responses. We report herein a contrasting role of interleukin-10 (IL-10) and Th2 cytokines in the regulation of OPN expression in human monocytes and monocyte-derived DCs (Mo-DCs). Our results showed first that the expression of OPN in monocytes and Mo-DCs was induced in a time-dependent and dose-dependent manner by IL-10 but was inhibited by IL-4 or IL-13. Further, the basal level of OPN expression was also inhibited by IL-4. This inhibitory effect of IL-4 was associated with a faster decay of OPN transcripts and a decreased proximal promoter activity of OPN in IL-4-treated cells. These results demonstrate a novel role of IL-10 and Th2 cytokines in the regulation of DC function through their contrasting regulatory activities on the expression of OPN.

Original languageEnglish
Pages (from-to)562-567
Number of pages6
JournalJournal of Interferon and Cytokine Research
Volume26
Issue number8
DOIs
Publication statusPublished - Aug 1 2006
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Virology

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