Interferon-gamma Genetic Polymorphism and Expression in Kawasaki Disease

Ho Chang Kuo, Ying Hsien Huang, Yu Wen Hsu, Hsing Fang Lu, Henry Sung Ching Wong, Hong Ren Yu, Hsing Chun Kuo, Fu Chen Huang, Wei Chiao Chang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Kawasaki disease (KD) is a systemic vasculitis of unknown etiology. IFNG gene encoding interferon (IFN)-γ, produced by natural killer cells and T cells, has been suggested to play an important role in the immunopathogenesis of Kawasaki disease. The aim of this study was to examin the correlation of gene polymorphisms of the IFNG gene and plasma levels of IFN-γ in KD patients and their outcomes. A total of 950 subjects (381 KD and 569 controls) were recruited. Three tagging single-nucleotide polymorphisms (rs2069718, rs1861493, rs2069705) were selected for TaqMan allelic discrimination assay. Clinical phenotypes, coronary artery lesions (CAL), coronary artery aneurysms (CAA) and intravenous immunoglobulin (IVIG) treatment outcomes were collected for analysis. Plasma IFN-γ levels were also measured with an enzyme-linked immunosorbent assay. Polymorphisms of the IFNG gene were significantly different between the normal controls and KD patients. The G allele of rs1861493 conferred a better response to IVIG treatment in KD patients. AA allele frequencies of rs1861493 were also associated with a significantly higher risk of CAA in KD patients. Furthermore, the plasma IFN-γ level was lower in the AA allele than in the GG allele of rs1861493 both before and after IVIG treatment in KD patients. This study provides the first evidence supporting an association between IFNG gene polymorphisms, susceptibility of KD, IVIG responsiveness, and plasma IFN-γ levels in KD patients.

Original languageEnglish
Article numbere3501
JournalMedicine (United States)
Volume95
Issue number17
DOIs
Publication statusPublished - Apr 1 2016

Fingerprint

Mucocutaneous Lymph Node Syndrome
Genetic Polymorphisms
Interferon-gamma
Interferons
Intravenous Immunoglobulins
Coronary Aneurysm
Coronary Vessels
Genes
Alleles
Systemic Vasculitis
Gene Frequency
Natural Killer Cells
Single Nucleotide Polymorphism
Enzyme-Linked Immunosorbent Assay
T-Lymphocytes
Phenotype

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Interferon-gamma Genetic Polymorphism and Expression in Kawasaki Disease. / Kuo, Ho Chang; Huang, Ying Hsien; Hsu, Yu Wen; Lu, Hsing Fang; Wong, Henry Sung Ching; Yu, Hong Ren; Kuo, Hsing Chun; Huang, Fu Chen; Chang, Wei Chiao.

In: Medicine (United States), Vol. 95, No. 17, e3501, 01.04.2016.

Research output: Contribution to journalArticle

Kuo, HC, Huang, YH, Hsu, YW, Lu, HF, Wong, HSC, Yu, HR, Kuo, HC, Huang, FC & Chang, WC 2016, 'Interferon-gamma Genetic Polymorphism and Expression in Kawasaki Disease', Medicine (United States), vol. 95, no. 17, e3501. https://doi.org/10.1097/MD.0000000000003501
Kuo, Ho Chang ; Huang, Ying Hsien ; Hsu, Yu Wen ; Lu, Hsing Fang ; Wong, Henry Sung Ching ; Yu, Hong Ren ; Kuo, Hsing Chun ; Huang, Fu Chen ; Chang, Wei Chiao. / Interferon-gamma Genetic Polymorphism and Expression in Kawasaki Disease. In: Medicine (United States). 2016 ; Vol. 95, No. 17.
@article{f5560b0c7dda41008722f66ba264d2ef,
title = "Interferon-gamma Genetic Polymorphism and Expression in Kawasaki Disease",
abstract = "Kawasaki disease (KD) is a systemic vasculitis of unknown etiology. IFNG gene encoding interferon (IFN)-γ, produced by natural killer cells and T cells, has been suggested to play an important role in the immunopathogenesis of Kawasaki disease. The aim of this study was to examin the correlation of gene polymorphisms of the IFNG gene and plasma levels of IFN-γ in KD patients and their outcomes. A total of 950 subjects (381 KD and 569 controls) were recruited. Three tagging single-nucleotide polymorphisms (rs2069718, rs1861493, rs2069705) were selected for TaqMan allelic discrimination assay. Clinical phenotypes, coronary artery lesions (CAL), coronary artery aneurysms (CAA) and intravenous immunoglobulin (IVIG) treatment outcomes were collected for analysis. Plasma IFN-γ levels were also measured with an enzyme-linked immunosorbent assay. Polymorphisms of the IFNG gene were significantly different between the normal controls and KD patients. The G allele of rs1861493 conferred a better response to IVIG treatment in KD patients. AA allele frequencies of rs1861493 were also associated with a significantly higher risk of CAA in KD patients. Furthermore, the plasma IFN-γ level was lower in the AA allele than in the GG allele of rs1861493 both before and after IVIG treatment in KD patients. This study provides the first evidence supporting an association between IFNG gene polymorphisms, susceptibility of KD, IVIG responsiveness, and plasma IFN-γ levels in KD patients.",
author = "Kuo, {Ho Chang} and Huang, {Ying Hsien} and Hsu, {Yu Wen} and Lu, {Hsing Fang} and Wong, {Henry Sung Ching} and Yu, {Hong Ren} and Kuo, {Hsing Chun} and Huang, {Fu Chen} and Chang, {Wei Chiao}",
year = "2016",
month = "4",
day = "1",
doi = "10.1097/MD.0000000000003501",
language = "English",
volume = "95",
journal = "Medicine; analytical reviews of general medicine, neurology, psychiatry, dermatology, and pediatries",
issn = "0025-7974",
publisher = "Lippincott Williams and Wilkins",
number = "17",

}

TY - JOUR

T1 - Interferon-gamma Genetic Polymorphism and Expression in Kawasaki Disease

AU - Kuo, Ho Chang

AU - Huang, Ying Hsien

AU - Hsu, Yu Wen

AU - Lu, Hsing Fang

AU - Wong, Henry Sung Ching

AU - Yu, Hong Ren

AU - Kuo, Hsing Chun

AU - Huang, Fu Chen

AU - Chang, Wei Chiao

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Kawasaki disease (KD) is a systemic vasculitis of unknown etiology. IFNG gene encoding interferon (IFN)-γ, produced by natural killer cells and T cells, has been suggested to play an important role in the immunopathogenesis of Kawasaki disease. The aim of this study was to examin the correlation of gene polymorphisms of the IFNG gene and plasma levels of IFN-γ in KD patients and their outcomes. A total of 950 subjects (381 KD and 569 controls) were recruited. Three tagging single-nucleotide polymorphisms (rs2069718, rs1861493, rs2069705) were selected for TaqMan allelic discrimination assay. Clinical phenotypes, coronary artery lesions (CAL), coronary artery aneurysms (CAA) and intravenous immunoglobulin (IVIG) treatment outcomes were collected for analysis. Plasma IFN-γ levels were also measured with an enzyme-linked immunosorbent assay. Polymorphisms of the IFNG gene were significantly different between the normal controls and KD patients. The G allele of rs1861493 conferred a better response to IVIG treatment in KD patients. AA allele frequencies of rs1861493 were also associated with a significantly higher risk of CAA in KD patients. Furthermore, the plasma IFN-γ level was lower in the AA allele than in the GG allele of rs1861493 both before and after IVIG treatment in KD patients. This study provides the first evidence supporting an association between IFNG gene polymorphisms, susceptibility of KD, IVIG responsiveness, and plasma IFN-γ levels in KD patients.

AB - Kawasaki disease (KD) is a systemic vasculitis of unknown etiology. IFNG gene encoding interferon (IFN)-γ, produced by natural killer cells and T cells, has been suggested to play an important role in the immunopathogenesis of Kawasaki disease. The aim of this study was to examin the correlation of gene polymorphisms of the IFNG gene and plasma levels of IFN-γ in KD patients and their outcomes. A total of 950 subjects (381 KD and 569 controls) were recruited. Three tagging single-nucleotide polymorphisms (rs2069718, rs1861493, rs2069705) were selected for TaqMan allelic discrimination assay. Clinical phenotypes, coronary artery lesions (CAL), coronary artery aneurysms (CAA) and intravenous immunoglobulin (IVIG) treatment outcomes were collected for analysis. Plasma IFN-γ levels were also measured with an enzyme-linked immunosorbent assay. Polymorphisms of the IFNG gene were significantly different between the normal controls and KD patients. The G allele of rs1861493 conferred a better response to IVIG treatment in KD patients. AA allele frequencies of rs1861493 were also associated with a significantly higher risk of CAA in KD patients. Furthermore, the plasma IFN-γ level was lower in the AA allele than in the GG allele of rs1861493 both before and after IVIG treatment in KD patients. This study provides the first evidence supporting an association between IFNG gene polymorphisms, susceptibility of KD, IVIG responsiveness, and plasma IFN-γ levels in KD patients.

UR - http://www.scopus.com/inward/record.url?scp=84978179927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978179927&partnerID=8YFLogxK

U2 - 10.1097/MD.0000000000003501

DO - 10.1097/MD.0000000000003501

M3 - Article

VL - 95

JO - Medicine; analytical reviews of general medicine, neurology, psychiatry, dermatology, and pediatries

JF - Medicine; analytical reviews of general medicine, neurology, psychiatry, dermatology, and pediatries

SN - 0025-7974

IS - 17

M1 - e3501

ER -