Interactions of aging and hydrogen peroxide on pulmonary vein electrical activity: Implications in the pathophysiology of atrial fibrillation

Yung Kuo Lin, Yao Chang Chen, Chen Chuan Cheng, Feng Zhi Lin, Cheng I. Lin, Shih Ann Chen, Yi Jen Chen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Aging and pulmonary veins (PVs) play critical roles in the pathophysiology of atrial fibrillation. Aging increases oxidative stress, which may induce atrial fibrillation. Since oxidative stress can increase PV arrhythmogenesis, the purpose of this study was to investigate whether aging may modulate the electrophysiological effects of oxidative stress on PVs. Methods and Results: Conventional microelectrodes were used to record the action potentials (APs) and contractility in isolated aged (age, 3 years) rabbit PV specimens before and after H2O2 administration. H 2O2 (0.02 and 0.2 mM) increased the aged PV spontaneous activity rates from 96 ± 12 to 108 ± 12 and 102 ± 12 beats/min (n = 9, p <0.05), respectively. In contrast, H2O 2 (2 mM) decreased aged PV spontaneous activity rates from 96 ± 12 to 72 ± 12 beats/min. H2O2 (0.02, 0.2 and 2 mM) induced PV burst firing (33%, 33%, and 67%) and early afterdepolarizations (11%, 33%, and 44%) in aged PVs with spontaneous activity (n = 6). In the aged PVs without spontaneous activity, H2O 2 (0.02, 0.2 and 2 mM) shortened the 90% of AP duration. H 2O2 at 2 mM, but not 0.02 or 0.2 mM, increased the contractile force. Conclusion: H2O2 modulates aged PV arrhythmogenesis, which may play a role in the genesis of atrial fibrillation.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalActa Cardiologica Sinica
Volume27
Issue number2
Publication statusPublished - Jun 2011

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Pulmonary Veins
Atrial Fibrillation
Hydrogen Peroxide
Oxidative Stress
Action Potentials
Microelectrodes
Rabbits

Keywords

  • Aging
  • Antioxidant
  • Atrial fibrillation
  • Hydrogen peroxide
  • Pulmonary vein

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Interactions of aging and hydrogen peroxide on pulmonary vein electrical activity : Implications in the pathophysiology of atrial fibrillation. / Lin, Yung Kuo; Chen, Yao Chang; Cheng, Chen Chuan; Lin, Feng Zhi; Lin, Cheng I.; Chen, Shih Ann; Chen, Yi Jen.

In: Acta Cardiologica Sinica, Vol. 27, No. 2, 06.2011, p. 109-114.

Research output: Contribution to journalArticle

Lin, Yung Kuo ; Chen, Yao Chang ; Cheng, Chen Chuan ; Lin, Feng Zhi ; Lin, Cheng I. ; Chen, Shih Ann ; Chen, Yi Jen. / Interactions of aging and hydrogen peroxide on pulmonary vein electrical activity : Implications in the pathophysiology of atrial fibrillation. In: Acta Cardiologica Sinica. 2011 ; Vol. 27, No. 2. pp. 109-114.
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abstract = "Background: Aging and pulmonary veins (PVs) play critical roles in the pathophysiology of atrial fibrillation. Aging increases oxidative stress, which may induce atrial fibrillation. Since oxidative stress can increase PV arrhythmogenesis, the purpose of this study was to investigate whether aging may modulate the electrophysiological effects of oxidative stress on PVs. Methods and Results: Conventional microelectrodes were used to record the action potentials (APs) and contractility in isolated aged (age, 3 years) rabbit PV specimens before and after H2O2 administration. H 2O2 (0.02 and 0.2 mM) increased the aged PV spontaneous activity rates from 96 ± 12 to 108 ± 12 and 102 ± 12 beats/min (n = 9, p <0.05), respectively. In contrast, H2O 2 (2 mM) decreased aged PV spontaneous activity rates from 96 ± 12 to 72 ± 12 beats/min. H2O2 (0.02, 0.2 and 2 mM) induced PV burst firing (33{\%}, 33{\%}, and 67{\%}) and early afterdepolarizations (11{\%}, 33{\%}, and 44{\%}) in aged PVs with spontaneous activity (n = 6). In the aged PVs without spontaneous activity, H2O 2 (0.02, 0.2 and 2 mM) shortened the 90{\%} of AP duration. H 2O2 at 2 mM, but not 0.02 or 0.2 mM, increased the contractile force. Conclusion: H2O2 modulates aged PV arrhythmogenesis, which may play a role in the genesis of atrial fibrillation.",
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N2 - Background: Aging and pulmonary veins (PVs) play critical roles in the pathophysiology of atrial fibrillation. Aging increases oxidative stress, which may induce atrial fibrillation. Since oxidative stress can increase PV arrhythmogenesis, the purpose of this study was to investigate whether aging may modulate the electrophysiological effects of oxidative stress on PVs. Methods and Results: Conventional microelectrodes were used to record the action potentials (APs) and contractility in isolated aged (age, 3 years) rabbit PV specimens before and after H2O2 administration. H 2O2 (0.02 and 0.2 mM) increased the aged PV spontaneous activity rates from 96 ± 12 to 108 ± 12 and 102 ± 12 beats/min (n = 9, p <0.05), respectively. In contrast, H2O 2 (2 mM) decreased aged PV spontaneous activity rates from 96 ± 12 to 72 ± 12 beats/min. H2O2 (0.02, 0.2 and 2 mM) induced PV burst firing (33%, 33%, and 67%) and early afterdepolarizations (11%, 33%, and 44%) in aged PVs with spontaneous activity (n = 6). In the aged PVs without spontaneous activity, H2O 2 (0.02, 0.2 and 2 mM) shortened the 90% of AP duration. H 2O2 at 2 mM, but not 0.02 or 0.2 mM, increased the contractile force. Conclusion: H2O2 modulates aged PV arrhythmogenesis, which may play a role in the genesis of atrial fibrillation.

AB - Background: Aging and pulmonary veins (PVs) play critical roles in the pathophysiology of atrial fibrillation. Aging increases oxidative stress, which may induce atrial fibrillation. Since oxidative stress can increase PV arrhythmogenesis, the purpose of this study was to investigate whether aging may modulate the electrophysiological effects of oxidative stress on PVs. Methods and Results: Conventional microelectrodes were used to record the action potentials (APs) and contractility in isolated aged (age, 3 years) rabbit PV specimens before and after H2O2 administration. H 2O2 (0.02 and 0.2 mM) increased the aged PV spontaneous activity rates from 96 ± 12 to 108 ± 12 and 102 ± 12 beats/min (n = 9, p <0.05), respectively. In contrast, H2O 2 (2 mM) decreased aged PV spontaneous activity rates from 96 ± 12 to 72 ± 12 beats/min. H2O2 (0.02, 0.2 and 2 mM) induced PV burst firing (33%, 33%, and 67%) and early afterdepolarizations (11%, 33%, and 44%) in aged PVs with spontaneous activity (n = 6). In the aged PVs without spontaneous activity, H2O 2 (0.02, 0.2 and 2 mM) shortened the 90% of AP duration. H 2O2 at 2 mM, but not 0.02 or 0.2 mM, increased the contractile force. Conclusion: H2O2 modulates aged PV arrhythmogenesis, which may play a role in the genesis of atrial fibrillation.

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