Interaction of estrogen and progesterone in the regulation of sodium channels in collecting tubular cells

Chiz Tzung Chang, Chiao Yin Sun, Chen Yan Pong, Yi Chang Chen, Geng Ping Lin, Tzu Ching Chang, Mai Szu Wu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Renal Na+ handling differs between males and females. Further, within females Na+ metabolism changes during the menstrual cycle and pregnancy. Estrogen and progesterone could regulate α, β and γ amiloride-sensitive epithelial sodium channel (ENaC) subunit mRNA levels in female rat kidney. The aim of our study is to clarify the role of the female gender steroids in the regulation of ENaC activity. Methods: We conducted an in vitro study on cultured collecting tubular cells. The collecting tubular cells were cultured under different concentrations of estrogen and/or progesterone. We analyzed the mRNA expression and protein translation of ENaC by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Sodium channel activity was measured by short circuit current. Results: Estrogen and/or progesterone alone mildly increased ENaC activity. A low concentration of estrogen together with progesterone stimulated ENaC activity more than 2-fold. A high concentration of estrogen almost completely inhibited the stimulated ENaC activity. Conclusion: Female gender steroid hormones affect RNA expression, protein translation and ENaC activity in renal collecting tubule cells. The effects might suggest that pre-menstruation edema is a result of subtle interaction between the female gender steroid hormones on ENaC in renal collecting tubule cells.

Original languageEnglish
Pages (from-to)305-312
Number of pages8
JournalChang Gung Medical Journal
Volume30
Issue number4
Publication statusPublished - Jul 2007
Externally publishedYes

Fingerprint

Sodium Channels
Progesterone
Estrogens
Protein Biosynthesis
Kidney
Steroids
Hormones
Epithelial Sodium Channels
Menstruation
Menstrual Cycle
Reverse Transcription
Cultured Cells
Edema
Western Blotting
RNA
Pregnancy
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Collecting tubular cell
  • Female gender steroid
  • Sodium channel

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Chang, C. T., Sun, C. Y., Pong, C. Y., Chen, Y. C., Lin, G. P., Chang, T. C., & Wu, M. S. (2007). Interaction of estrogen and progesterone in the regulation of sodium channels in collecting tubular cells. Chang Gung Medical Journal, 30(4), 305-312.

Interaction of estrogen and progesterone in the regulation of sodium channels in collecting tubular cells. / Chang, Chiz Tzung; Sun, Chiao Yin; Pong, Chen Yan; Chen, Yi Chang; Lin, Geng Ping; Chang, Tzu Ching; Wu, Mai Szu.

In: Chang Gung Medical Journal, Vol. 30, No. 4, 07.2007, p. 305-312.

Research output: Contribution to journalArticle

Chang, CT, Sun, CY, Pong, CY, Chen, YC, Lin, GP, Chang, TC & Wu, MS 2007, 'Interaction of estrogen and progesterone in the regulation of sodium channels in collecting tubular cells', Chang Gung Medical Journal, vol. 30, no. 4, pp. 305-312.
Chang, Chiz Tzung ; Sun, Chiao Yin ; Pong, Chen Yan ; Chen, Yi Chang ; Lin, Geng Ping ; Chang, Tzu Ching ; Wu, Mai Szu. / Interaction of estrogen and progesterone in the regulation of sodium channels in collecting tubular cells. In: Chang Gung Medical Journal. 2007 ; Vol. 30, No. 4. pp. 305-312.
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