Interaction effects of ultrafine carbon black with iron and nickel on heart rate variability in spontaneously hypertensive rats

Chuen-Chau Chang, Jing Shiang Hwang, Chang Chuan Chan, Tsun J. Cheng

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: Particulate matter (PM) has been reported to be associated with alterations in heart rate variability (HRV); however, the results are inconsistent. We propose that different components of PM cause the discrepancy. Objective: In this study, our goal was to determine whether different types exposure would cause different HRV effects, and to verify the interactions between co-exposing components. Methods: Ultrafine carbon black (ufCB; 14 nm; 415 μg and 830 μg), ferric sulfate [Fe2(SO4)3; 105 μg and 210 μg], nickel sulfate (NiSO4; 263 μg and 526 μg), and a combination of high-dose ufCB and low-dose Fe2(SO4)3 or NiSO4 were intratracheally instilled into spontaneously hypertensive rats. Radiotelemetry data were collected in rats for 72 hr at baseline and for 72 hr the following week to determine the response to exposure. Effects of exposure on 5-min average of normal-to-normal intervals (ANN), natural logarithm-transformed standard deviation of the normal-to-normal intervals (LnSDNN), and root mean square of successive differences of adjacent normal-to-normal intervals (LnRMSSD) were analyzed using self-control experimental designs. Results: Both high-and low-dose nfCB decreased ANN marginally around hour 30, with concurrent increases of LnSDNN. LnRMSSD returned to baseline levels after small initial increases. We observed minor effects after low-dose Fe and Ni instillation, whereas biphasic changes were noted after high-dose instillations. Combined exposures of ufCB and either Fe or Ni resulted in HRV trends different from values estimated from individual-component effects. Conclusions: Components in PM may induce different cardioregulatory responses, and a single component may induce different responses during different phases. Concurrent exposure to ufCB and Fe or Ni might introduce interactions on cardioregulatory effects. Also, the effect of PM may be mediated through complex interaction between different components of PM.

Original languageEnglish
Pages (from-to)1012-1017
Number of pages6
JournalEnvironmental Health Perspectives
Volume115
Issue number7
DOIs
Publication statusPublished - Jul 2007

Fingerprint

Soot
Particulate Matter
Inbred SHR Rats
black carbon
Nickel
Rats
nickel
Iron
Heart Rate
particulate matter
iron
sulfate
radiotelemetry
Design of experiments
Research Design
experimental design
Ultrafine
rate
effect
dose

Keywords

  • Ambient particles
  • Heart rate variability
  • Interaction
  • Iron
  • Nickel
  • Spontaneously hypertensive rats
  • Ultrafine carbon black

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Interaction effects of ultrafine carbon black with iron and nickel on heart rate variability in spontaneously hypertensive rats. / Chang, Chuen-Chau; Hwang, Jing Shiang; Chan, Chang Chuan; Cheng, Tsun J.

In: Environmental Health Perspectives, Vol. 115, No. 7, 07.2007, p. 1012-1017.

Research output: Contribution to journalArticle

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abstract = "Background: Particulate matter (PM) has been reported to be associated with alterations in heart rate variability (HRV); however, the results are inconsistent. We propose that different components of PM cause the discrepancy. Objective: In this study, our goal was to determine whether different types exposure would cause different HRV effects, and to verify the interactions between co-exposing components. Methods: Ultrafine carbon black (ufCB; 14 nm; 415 μg and 830 μg), ferric sulfate [Fe2(SO4)3; 105 μg and 210 μg], nickel sulfate (NiSO4; 263 μg and 526 μg), and a combination of high-dose ufCB and low-dose Fe2(SO4)3 or NiSO4 were intratracheally instilled into spontaneously hypertensive rats. Radiotelemetry data were collected in rats for 72 hr at baseline and for 72 hr the following week to determine the response to exposure. Effects of exposure on 5-min average of normal-to-normal intervals (ANN), natural logarithm-transformed standard deviation of the normal-to-normal intervals (LnSDNN), and root mean square of successive differences of adjacent normal-to-normal intervals (LnRMSSD) were analyzed using self-control experimental designs. Results: Both high-and low-dose nfCB decreased ANN marginally around hour 30, with concurrent increases of LnSDNN. LnRMSSD returned to baseline levels after small initial increases. We observed minor effects after low-dose Fe and Ni instillation, whereas biphasic changes were noted after high-dose instillations. Combined exposures of ufCB and either Fe or Ni resulted in HRV trends different from values estimated from individual-component effects. Conclusions: Components in PM may induce different cardioregulatory responses, and a single component may induce different responses during different phases. Concurrent exposure to ufCB and Fe or Ni might introduce interactions on cardioregulatory effects. Also, the effect of PM may be mediated through complex interaction between different components of PM.",
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N2 - Background: Particulate matter (PM) has been reported to be associated with alterations in heart rate variability (HRV); however, the results are inconsistent. We propose that different components of PM cause the discrepancy. Objective: In this study, our goal was to determine whether different types exposure would cause different HRV effects, and to verify the interactions between co-exposing components. Methods: Ultrafine carbon black (ufCB; 14 nm; 415 μg and 830 μg), ferric sulfate [Fe2(SO4)3; 105 μg and 210 μg], nickel sulfate (NiSO4; 263 μg and 526 μg), and a combination of high-dose ufCB and low-dose Fe2(SO4)3 or NiSO4 were intratracheally instilled into spontaneously hypertensive rats. Radiotelemetry data were collected in rats for 72 hr at baseline and for 72 hr the following week to determine the response to exposure. Effects of exposure on 5-min average of normal-to-normal intervals (ANN), natural logarithm-transformed standard deviation of the normal-to-normal intervals (LnSDNN), and root mean square of successive differences of adjacent normal-to-normal intervals (LnRMSSD) were analyzed using self-control experimental designs. Results: Both high-and low-dose nfCB decreased ANN marginally around hour 30, with concurrent increases of LnSDNN. LnRMSSD returned to baseline levels after small initial increases. We observed minor effects after low-dose Fe and Ni instillation, whereas biphasic changes were noted after high-dose instillations. Combined exposures of ufCB and either Fe or Ni resulted in HRV trends different from values estimated from individual-component effects. Conclusions: Components in PM may induce different cardioregulatory responses, and a single component may induce different responses during different phases. Concurrent exposure to ufCB and Fe or Ni might introduce interactions on cardioregulatory effects. Also, the effect of PM may be mediated through complex interaction between different components of PM.

AB - Background: Particulate matter (PM) has been reported to be associated with alterations in heart rate variability (HRV); however, the results are inconsistent. We propose that different components of PM cause the discrepancy. Objective: In this study, our goal was to determine whether different types exposure would cause different HRV effects, and to verify the interactions between co-exposing components. Methods: Ultrafine carbon black (ufCB; 14 nm; 415 μg and 830 μg), ferric sulfate [Fe2(SO4)3; 105 μg and 210 μg], nickel sulfate (NiSO4; 263 μg and 526 μg), and a combination of high-dose ufCB and low-dose Fe2(SO4)3 or NiSO4 were intratracheally instilled into spontaneously hypertensive rats. Radiotelemetry data were collected in rats for 72 hr at baseline and for 72 hr the following week to determine the response to exposure. Effects of exposure on 5-min average of normal-to-normal intervals (ANN), natural logarithm-transformed standard deviation of the normal-to-normal intervals (LnSDNN), and root mean square of successive differences of adjacent normal-to-normal intervals (LnRMSSD) were analyzed using self-control experimental designs. Results: Both high-and low-dose nfCB decreased ANN marginally around hour 30, with concurrent increases of LnSDNN. LnRMSSD returned to baseline levels after small initial increases. We observed minor effects after low-dose Fe and Ni instillation, whereas biphasic changes were noted after high-dose instillations. Combined exposures of ufCB and either Fe or Ni resulted in HRV trends different from values estimated from individual-component effects. Conclusions: Components in PM may induce different cardioregulatory responses, and a single component may induce different responses during different phases. Concurrent exposure to ufCB and Fe or Ni might introduce interactions on cardioregulatory effects. Also, the effect of PM may be mediated through complex interaction between different components of PM.

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KW - Nickel

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KW - Ultrafine carbon black

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