Interaction between human topoisomerase I and a novel RING finger/arginine-serine protein

Paul Haluska, Ahamed Saleem, Zeshaan Rasheed, Farheena Ahmed, Emily W. Su, Leroy-Fong Liu, Eric H. Rubin

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The N-terminus of human topoisomerase I participates in the binding of this enzyme to helicases and other proteins. Using the N-terminal 250 amino acids of human topoisomerase I and a yeast two-hybrid/ in vitro binding screen, a novel arginine-serine-rich peptide was identified as a human topoisomerase I-binding protein. The corresponding full-length protein, named topors, contains a consensus RING zinc finger domain and nuclear localization signals in addition to the arginine-serine-rich region. The RING finger domain of topors is homologous to a similar domain in a family of viral proteins that are involved in the regulation of viral transcription. When expressed in HeLa cells as a green fluorescent protein fusion, topors localizes in the nucleus in a punctate pattern and co-immunoprecipitates with topoisomerase I. These data suggest that topors is involved in transcription, possibly recruiting topoisomerase I to RNA polymerase II transcriptional complexes.

Original languageEnglish
Pages (from-to)2538-2544
Number of pages7
JournalNucleic Acids Research
Volume27
Issue number12
DOIs
Publication statusPublished - Jun 15 1999
Externally publishedYes

Fingerprint

Type I DNA Topoisomerase
Serine
Arginine
RING Finger Domains
Proteins
Nuclear Localization Signals
RNA Polymerase II
Zinc Fingers
Viral Proteins
Green Fluorescent Proteins
HeLa Cells
Carrier Proteins
Yeasts
Amino Acids
Peptides
Enzymes

ASJC Scopus subject areas

  • Genetics

Cite this

Interaction between human topoisomerase I and a novel RING finger/arginine-serine protein. / Haluska, Paul; Saleem, Ahamed; Rasheed, Zeshaan; Ahmed, Farheena; Su, Emily W.; Liu, Leroy-Fong; Rubin, Eric H.

In: Nucleic Acids Research, Vol. 27, No. 12, 15.06.1999, p. 2538-2544.

Research output: Contribution to journalArticle

Haluska, P, Saleem, A, Rasheed, Z, Ahmed, F, Su, EW, Liu, L-F & Rubin, EH 1999, 'Interaction between human topoisomerase I and a novel RING finger/arginine-serine protein', Nucleic Acids Research, vol. 27, no. 12, pp. 2538-2544. https://doi.org/10.1093/nar/27.12.2538
Haluska, Paul ; Saleem, Ahamed ; Rasheed, Zeshaan ; Ahmed, Farheena ; Su, Emily W. ; Liu, Leroy-Fong ; Rubin, Eric H. / Interaction between human topoisomerase I and a novel RING finger/arginine-serine protein. In: Nucleic Acids Research. 1999 ; Vol. 27, No. 12. pp. 2538-2544.
@article{58ed88e4f8a347f3bfe60f8d81abc445,
title = "Interaction between human topoisomerase I and a novel RING finger/arginine-serine protein",
abstract = "The N-terminus of human topoisomerase I participates in the binding of this enzyme to helicases and other proteins. Using the N-terminal 250 amino acids of human topoisomerase I and a yeast two-hybrid/ in vitro binding screen, a novel arginine-serine-rich peptide was identified as a human topoisomerase I-binding protein. The corresponding full-length protein, named topors, contains a consensus RING zinc finger domain and nuclear localization signals in addition to the arginine-serine-rich region. The RING finger domain of topors is homologous to a similar domain in a family of viral proteins that are involved in the regulation of viral transcription. When expressed in HeLa cells as a green fluorescent protein fusion, topors localizes in the nucleus in a punctate pattern and co-immunoprecipitates with topoisomerase I. These data suggest that topors is involved in transcription, possibly recruiting topoisomerase I to RNA polymerase II transcriptional complexes.",
author = "Paul Haluska and Ahamed Saleem and Zeshaan Rasheed and Farheena Ahmed and Su, {Emily W.} and Leroy-Fong Liu and Rubin, {Eric H.}",
year = "1999",
month = "6",
day = "15",
doi = "10.1093/nar/27.12.2538",
language = "English",
volume = "27",
pages = "2538--2544",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "12",

}

TY - JOUR

T1 - Interaction between human topoisomerase I and a novel RING finger/arginine-serine protein

AU - Haluska, Paul

AU - Saleem, Ahamed

AU - Rasheed, Zeshaan

AU - Ahmed, Farheena

AU - Su, Emily W.

AU - Liu, Leroy-Fong

AU - Rubin, Eric H.

PY - 1999/6/15

Y1 - 1999/6/15

N2 - The N-terminus of human topoisomerase I participates in the binding of this enzyme to helicases and other proteins. Using the N-terminal 250 amino acids of human topoisomerase I and a yeast two-hybrid/ in vitro binding screen, a novel arginine-serine-rich peptide was identified as a human topoisomerase I-binding protein. The corresponding full-length protein, named topors, contains a consensus RING zinc finger domain and nuclear localization signals in addition to the arginine-serine-rich region. The RING finger domain of topors is homologous to a similar domain in a family of viral proteins that are involved in the regulation of viral transcription. When expressed in HeLa cells as a green fluorescent protein fusion, topors localizes in the nucleus in a punctate pattern and co-immunoprecipitates with topoisomerase I. These data suggest that topors is involved in transcription, possibly recruiting topoisomerase I to RNA polymerase II transcriptional complexes.

AB - The N-terminus of human topoisomerase I participates in the binding of this enzyme to helicases and other proteins. Using the N-terminal 250 amino acids of human topoisomerase I and a yeast two-hybrid/ in vitro binding screen, a novel arginine-serine-rich peptide was identified as a human topoisomerase I-binding protein. The corresponding full-length protein, named topors, contains a consensus RING zinc finger domain and nuclear localization signals in addition to the arginine-serine-rich region. The RING finger domain of topors is homologous to a similar domain in a family of viral proteins that are involved in the regulation of viral transcription. When expressed in HeLa cells as a green fluorescent protein fusion, topors localizes in the nucleus in a punctate pattern and co-immunoprecipitates with topoisomerase I. These data suggest that topors is involved in transcription, possibly recruiting topoisomerase I to RNA polymerase II transcriptional complexes.

UR - http://www.scopus.com/inward/record.url?scp=0345298272&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345298272&partnerID=8YFLogxK

U2 - 10.1093/nar/27.12.2538

DO - 10.1093/nar/27.12.2538

M3 - Article

C2 - 10352183

AN - SCOPUS:0345298272

VL - 27

SP - 2538

EP - 2544

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 12

ER -