Integron-associated imipenem resistance in Acinetobacter baumannii isolated from a regional hospital in Taiwan

S. Y. Liu, J. Y. Lin, C. Chu, L. H. Su, T. Y. Lin, C. H. Chiu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We investigated the genetic properties of imipenem-resistant Acinetobacter baumannii collected from a regional hospital in Taiwan. Pulsed-field gel electrophoresis demonstrated that the isolates were genetically diverse. Polymerase chain reaction, DNA sequencing, and DNA-DNA hybridisation showed that the blaIMP-1 gene resided as a cassette in a plasmid-borne class 1 integron in two isolates. The majority of the resistant isolates were plasmid-less and carried no blaIMP, blaVIM or bla CFI genes, indicating that other uncharacterised metallo-β- lactamases or mechanisms other than enzyme production are involved in carbapenem resistance in this group of A. baumannii. We conclude that multidrug resistance of A. baumannii was a combined effect of lateral gene transfer and clonal spread of multiple resistant clones. Strict measures should be implemented to control the further spread of resistance.

Original languageEnglish
Pages (from-to)81-84
Number of pages4
JournalInternational Journal of Antimicrobial Agents
Volume27
Issue number1
DOIs
Publication statusPublished - Jan 2006
Externally publishedYes

Fingerprint

Integrons
Acinetobacter baumannii
Imipenem
Taiwan
Plasmids
Horizontal Gene Transfer
Carbapenems
Pulsed Field Gel Electrophoresis
DNA
Multiple Drug Resistance
DNA Sequence Analysis
Genes
Clone Cells
Polymerase Chain Reaction
Enzymes

Keywords

  • Acinetobacter baumannii
  • Imipenem resistance
  • Integron

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Integron-associated imipenem resistance in Acinetobacter baumannii isolated from a regional hospital in Taiwan. / Liu, S. Y.; Lin, J. Y.; Chu, C.; Su, L. H.; Lin, T. Y.; Chiu, C. H.

In: International Journal of Antimicrobial Agents, Vol. 27, No. 1, 01.2006, p. 81-84.

Research output: Contribution to journalArticle

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