Integrins α2β1 and α4β1 can mediate SA11 rotavirus attachment and entry into cells

Marilyn J. Hewish, Yoshikazu Takada, Barbara S. Coulson

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Most mammalian rotaviruses contain tripeptide amino acid sequences in outer capsid proteins VP4 and VP7 which have been shown to act as ligands for integrins α2β1 and α4β1. Peptides containing these sequences and monoclonal antibodies directed to these integrins block rotavirus infection of cells. Here we report that SAIl rotavirus binding to and infection of K562 cells expressing α2β1 or α4β1 integrins via transfection is increased over virus binding to and infection of cells transfected with α3 integrin or parent cells. The increased binding and growth were specifically blocked by a monoclonal antibody to the transfected integrin subunit but not by irrelevant antibodies. In our experiments, integrin activation with phorbol ester did not affect virus binding to cells. However, phorbol ester treatment of K562 parent and transfected cells induced endogenous gene expression of α2β1 integrin, which was detectable by flow cytometry 16 h after treatment and quantitatively correlated with the increased level of SAIl virus growth observed after this time. Virus binding to K562 cells treated with phorbol ester 24 h previously and expressing α2β1 was elevated over binding to control cells and was specifically blocked by the anti-α2 monoclonal antibody AK7. Virus growth in α4-transfected K562 cells which had also been induced to express α2β1 integrin with phorbol ester occurred at a level approaching that in the permissive MA104 cell line. We therefore have demonstrated that two integrins, α2β1 and α4β1, are capable of acting as cellular receptors for SA11 rotavirus.

Original languageEnglish
Pages (from-to)228-236
Number of pages9
JournalJournal of Virology
Volume74
Issue number1
Publication statusPublished - 2000
Externally publishedYes

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Rotavirus
integrins
Integrins
Phorbol Esters
Virus Attachment
K562 Cells
cells
viruses
esters
monoclonal antibodies
Monoclonal Antibodies
Growth
infection
Viruses
Rotavirus Infections
tripeptides
Capsid Proteins
Virus Diseases
transfection
coat proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Hewish, M. J., Takada, Y., & Coulson, B. S. (2000). Integrins α2β1 and α4β1 can mediate SA11 rotavirus attachment and entry into cells. Journal of Virology, 74(1), 228-236.

Integrins α2β1 and α4β1 can mediate SA11 rotavirus attachment and entry into cells. / Hewish, Marilyn J.; Takada, Yoshikazu; Coulson, Barbara S.

In: Journal of Virology, Vol. 74, No. 1, 2000, p. 228-236.

Research output: Contribution to journalArticle

Hewish, MJ, Takada, Y & Coulson, BS 2000, 'Integrins α2β1 and α4β1 can mediate SA11 rotavirus attachment and entry into cells', Journal of Virology, vol. 74, no. 1, pp. 228-236.
Hewish, Marilyn J. ; Takada, Yoshikazu ; Coulson, Barbara S. / Integrins α2β1 and α4β1 can mediate SA11 rotavirus attachment and entry into cells. In: Journal of Virology. 2000 ; Vol. 74, No. 1. pp. 228-236.
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