Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment

Li-Chern Pan, Fang Chi Hsu, Yun Ying Wu, Fan Gang Tseng, Da Jen Yao, Yieh Loong Tsai, Jiann-Loung Hwang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Since male abnormality has now been reported to account at least thirty percent of couples with fertility problem. Therefore, distinguishing and classification of sperm has become more and more important, especially when Assisted Reproductive Technology (ART) like in vitro fertilization (IVF) is involved. However, traditional sperm sorting methods all requires manual selection, which is tedious and often cause sperm damage during the screen process. Although, new design from our recent study, seemed to point for a possibility to make these kind of MISS device capable of separating sperms into various mobility sub-class. In addition, the Hoechst 33258 and propidium iodide double staining results, also confirmed that the most motile and health sperm is indeed able to leave the laminar stream and being sorted out at outlet-h, with least dead sperm or debris. Meanwhile, we observed the dead sperm had been dominate from clear red stains and live sperm are not as active as is corresponded with more diffused blue stains. In order to confirm the real sorting situation, we counted the amount of sperm in collected sample by flow cytometry and tried to establish the statistic model. Though the flow cytometry, we could detect the sperm fluorescent distribution and count the size of sperm population. Nevertheless, t-test with 95% confidence level also shown significant difference in live/dead sperm ratio. Therefore, from the combined result among flow cytometry, fluorescent stain, and the statistic model test, it is confirmed that live sperms were able to cross the laminar stream created by the device and reach the sorting chamber with corresponding range from non-motile sperms to debris, as well as from living to dead ones. It is concluded that the proposed method is cost effective and is possible to serve as a protocol for rapid sperm quantitative assessment.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages332-335
Number of pages4
Volume25
Edition8
DOIs
Publication statusPublished - 2009
EventWorld Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors - Munich, Germany
Duration: Sep 7 2009Sep 12 2009

Other

OtherWorld Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors
CountryGermany
CityMunich
Period9/7/099/12/09

Fingerprint

Flow cytometry
Fluidics
Sorting
Coloring Agents
Debris
Statistics
Bisbenzimidazole
Propidium
Health
Costs

Keywords

  • Fluorescence
  • Micro fluidic device
  • Sperm motility

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Pan, L-C., Hsu, F. C., Wu, Y. Y., Tseng, F. G., Yao, D. J., Tsai, Y. L., & Hwang, J-L. (2009). Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment. In IFMBE Proceedings (8 ed., Vol. 25, pp. 332-335) https://doi.org/10.1007/978-3-642-03887-7-96

Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment. / Pan, Li-Chern; Hsu, Fang Chi; Wu, Yun Ying; Tseng, Fan Gang; Yao, Da Jen; Tsai, Yieh Loong; Hwang, Jiann-Loung.

IFMBE Proceedings. Vol. 25 8. ed. 2009. p. 332-335.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pan, L-C, Hsu, FC, Wu, YY, Tseng, FG, Yao, DJ, Tsai, YL & Hwang, J-L 2009, Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment. in IFMBE Proceedings. 8 edn, vol. 25, pp. 332-335, World Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors, Munich, Germany, 9/7/09. https://doi.org/10.1007/978-3-642-03887-7-96
Pan, Li-Chern ; Hsu, Fang Chi ; Wu, Yun Ying ; Tseng, Fan Gang ; Yao, Da Jen ; Tsai, Yieh Loong ; Hwang, Jiann-Loung. / Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment. IFMBE Proceedings. Vol. 25 8. ed. 2009. pp. 332-335
@inproceedings{fe3b1df1b3704b42b12c477d5860aa3a,
title = "Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment",
abstract = "Since male abnormality has now been reported to account at least thirty percent of couples with fertility problem. Therefore, distinguishing and classification of sperm has become more and more important, especially when Assisted Reproductive Technology (ART) like in vitro fertilization (IVF) is involved. However, traditional sperm sorting methods all requires manual selection, which is tedious and often cause sperm damage during the screen process. Although, new design from our recent study, seemed to point for a possibility to make these kind of MISS device capable of separating sperms into various mobility sub-class. In addition, the Hoechst 33258 and propidium iodide double staining results, also confirmed that the most motile and health sperm is indeed able to leave the laminar stream and being sorted out at outlet-h, with least dead sperm or debris. Meanwhile, we observed the dead sperm had been dominate from clear red stains and live sperm are not as active as is corresponded with more diffused blue stains. In order to confirm the real sorting situation, we counted the amount of sperm in collected sample by flow cytometry and tried to establish the statistic model. Though the flow cytometry, we could detect the sperm fluorescent distribution and count the size of sperm population. Nevertheless, t-test with 95{\%} confidence level also shown significant difference in live/dead sperm ratio. Therefore, from the combined result among flow cytometry, fluorescent stain, and the statistic model test, it is confirmed that live sperms were able to cross the laminar stream created by the device and reach the sorting chamber with corresponding range from non-motile sperms to debris, as well as from living to dead ones. It is concluded that the proposed method is cost effective and is possible to serve as a protocol for rapid sperm quantitative assessment.",
keywords = "Fluorescence, Micro fluidic device, Sperm motility",
author = "Li-Chern Pan and Hsu, {Fang Chi} and Wu, {Yun Ying} and Tseng, {Fan Gang} and Yao, {Da Jen} and Tsai, {Yieh Loong} and Jiann-Loung Hwang",
year = "2009",
doi = "10.1007/978-3-642-03887-7-96",
language = "English",
isbn = "9783642038860",
volume = "25",
pages = "332--335",
booktitle = "IFMBE Proceedings",
edition = "8",

}

TY - GEN

T1 - Integration of micro fluidic bio-chip design and automatic fluorescent identification for rapid sperm mobility assessment

AU - Pan, Li-Chern

AU - Hsu, Fang Chi

AU - Wu, Yun Ying

AU - Tseng, Fan Gang

AU - Yao, Da Jen

AU - Tsai, Yieh Loong

AU - Hwang, Jiann-Loung

PY - 2009

Y1 - 2009

N2 - Since male abnormality has now been reported to account at least thirty percent of couples with fertility problem. Therefore, distinguishing and classification of sperm has become more and more important, especially when Assisted Reproductive Technology (ART) like in vitro fertilization (IVF) is involved. However, traditional sperm sorting methods all requires manual selection, which is tedious and often cause sperm damage during the screen process. Although, new design from our recent study, seemed to point for a possibility to make these kind of MISS device capable of separating sperms into various mobility sub-class. In addition, the Hoechst 33258 and propidium iodide double staining results, also confirmed that the most motile and health sperm is indeed able to leave the laminar stream and being sorted out at outlet-h, with least dead sperm or debris. Meanwhile, we observed the dead sperm had been dominate from clear red stains and live sperm are not as active as is corresponded with more diffused blue stains. In order to confirm the real sorting situation, we counted the amount of sperm in collected sample by flow cytometry and tried to establish the statistic model. Though the flow cytometry, we could detect the sperm fluorescent distribution and count the size of sperm population. Nevertheless, t-test with 95% confidence level also shown significant difference in live/dead sperm ratio. Therefore, from the combined result among flow cytometry, fluorescent stain, and the statistic model test, it is confirmed that live sperms were able to cross the laminar stream created by the device and reach the sorting chamber with corresponding range from non-motile sperms to debris, as well as from living to dead ones. It is concluded that the proposed method is cost effective and is possible to serve as a protocol for rapid sperm quantitative assessment.

AB - Since male abnormality has now been reported to account at least thirty percent of couples with fertility problem. Therefore, distinguishing and classification of sperm has become more and more important, especially when Assisted Reproductive Technology (ART) like in vitro fertilization (IVF) is involved. However, traditional sperm sorting methods all requires manual selection, which is tedious and often cause sperm damage during the screen process. Although, new design from our recent study, seemed to point for a possibility to make these kind of MISS device capable of separating sperms into various mobility sub-class. In addition, the Hoechst 33258 and propidium iodide double staining results, also confirmed that the most motile and health sperm is indeed able to leave the laminar stream and being sorted out at outlet-h, with least dead sperm or debris. Meanwhile, we observed the dead sperm had been dominate from clear red stains and live sperm are not as active as is corresponded with more diffused blue stains. In order to confirm the real sorting situation, we counted the amount of sperm in collected sample by flow cytometry and tried to establish the statistic model. Though the flow cytometry, we could detect the sperm fluorescent distribution and count the size of sperm population. Nevertheless, t-test with 95% confidence level also shown significant difference in live/dead sperm ratio. Therefore, from the combined result among flow cytometry, fluorescent stain, and the statistic model test, it is confirmed that live sperms were able to cross the laminar stream created by the device and reach the sorting chamber with corresponding range from non-motile sperms to debris, as well as from living to dead ones. It is concluded that the proposed method is cost effective and is possible to serve as a protocol for rapid sperm quantitative assessment.

KW - Fluorescence

KW - Micro fluidic device

KW - Sperm motility

UR - http://www.scopus.com/inward/record.url?scp=77949894503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77949894503&partnerID=8YFLogxK

U2 - 10.1007/978-3-642-03887-7-96

DO - 10.1007/978-3-642-03887-7-96

M3 - Conference contribution

AN - SCOPUS:77949894503

SN - 9783642038860

VL - 25

SP - 332

EP - 335

BT - IFMBE Proceedings

ER -