Integration of independent component analysis with near-infrared spectroscopy for analysis of bioactive components in the medicinal plant Gentiana scabra Bunge

Yung Kun Chuang, I. Chang Yang, Yangming Martin Lo, Chao Yin Tsai, Suming Chen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Independent component (IC) analysis was applied to near-infrared spectroscopy for analysis of gentiopicroside and swertiamarin; the two bioactive components of Gentiana scabra Bunge. ICs that are highly correlated with the two bioactive components were selected for the analysis of tissue cultures, shoots and roots, which were found to distribute in three different positions within the domain [two-dimensional (2D) and 3D] constructed by the ICs. This setup could be used for quantitative determination of respective contents of gentiopicroside and swertiamarin within the plants. For gentiopicroside, the spectral calibration model based on the second derivative spectra produced the best effect in the wavelength ranges of 600-700 nm, 1600-1700 nm, and 2000-2300 nm (correlation coefficient of calibration = 0.847, standard error of calibration = 0.865%, and standard error of validation = 0.909%). For swertiamarin, a spectral calibration model based on the first derivative spectra produced the best effect in the wavelength ranges of 600-800 nm and 2200-2300 nm (correlation coefficient of calibration = 0.948, standard error of calibration = 0.168%, and standard error of validation = 0.216%). Both models showed a satisfactory predictability. This study successfully established qualitative and quantitative correlations for gentiopicroside and swertiamarin with near-infrared spectra, enabling rapid and accurate inspection on the bioactive components of G. scabra Bunge at different growth stages.

Original languageEnglish
Pages (from-to)336-344
Number of pages9
JournalJournal of Food and Drug Analysis
Volume22
Issue number3
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Gentiana scabra Bunge
  • Gentiopicroside
  • Independent component analysis
  • Near-infrared spectroscopy
  • Swertiamarin

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

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