Integration of human papillomavirus correlates with high levels of viral oncogene transcripts in cervical carcinogenesis

Chih Ming Ho, Bor Heng Lee, Shwu Fen Chang, Tsai Yen Chien, Shih Hung Huang, Chiu Cho Yan, Weng Fang Cheng

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A prospective, cross-sectional study was conducted to investigate the correlation between the integration of high-risk human papillomavirus and disease severity of cervical lesions. 720 liquid-based cytology specimens including 422 normal cytology, 78 low-grade squamous intraepithelial lesions, 172 high-grade squamous intraepithelial lesions, and 48 women with cervical cancers were examined using HPV blot and type-specific E6 PCR. Positive HPV DNA types 16, 18, 52 and 58 were examined for viral DNA using real-time PCR. Expression of E6 transcripts was 89.5% (pure integration), 71.7% (mixed type), and 47.1% (pure episomal) (p<0.0001). Geometric mean levels ranged from 110.6 (episomal form) to 508.4 (mixed form), and 5966.2 (integration form) by real-time PCR (p<0.0001). Geometric mean levels of E6 transcript in HPV 16, 18, 52, and 58 correlated with the severity of cervical lesions and the physical integration state of the viral genome (p<0.0001). We conclude that this is the first paper to point out that integration of high-risk HPVs not only 16 and 18 but also 52 and 58 is correlated with high levels of oncogene transcripts from normal cervix, CIN to cervical cancer.

Original languageEnglish
Pages (from-to)124-130
Number of pages7
JournalVirus Research
Volume161
Issue number2
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Human papillomavirus 16
Oncogenes
Uterine Cervical Neoplasms
Cell Biology
Real-Time Polymerase Chain Reaction
Carcinogenesis
Human papillomavirus 18
Viral Genome
Viral DNA
Cervix Uteri
Cross-Sectional Studies
Polymerase Chain Reaction
DNA
Squamous Intraepithelial Lesions of the Cervix

Keywords

  • Cervical cancer
  • Human papillomavirus
  • Integration
  • Physical state

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Integration of human papillomavirus correlates with high levels of viral oncogene transcripts in cervical carcinogenesis. / Ho, Chih Ming; Lee, Bor Heng; Chang, Shwu Fen; Chien, Tsai Yen; Huang, Shih Hung; Yan, Chiu Cho; Cheng, Weng Fang.

In: Virus Research, Vol. 161, No. 2, 11.2011, p. 124-130.

Research output: Contribution to journalArticle

Ho, Chih Ming ; Lee, Bor Heng ; Chang, Shwu Fen ; Chien, Tsai Yen ; Huang, Shih Hung ; Yan, Chiu Cho ; Cheng, Weng Fang. / Integration of human papillomavirus correlates with high levels of viral oncogene transcripts in cervical carcinogenesis. In: Virus Research. 2011 ; Vol. 161, No. 2. pp. 124-130.
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