Integration of high-risk human papillomavirus DNA correlates with HLA genotype aberration and reduced HLA class I molecule expression in human cervical carcinoma

Bor Ching Sheu, Shin Heng Chiou, Wen Chun Chang, Song Nan Chow, Ho Hsiung Lin, Ruey Jien Chen, Su Cheng Huang, Hong Nerng Ho, Su Ming Hsu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In human cervical cancer (CC), local immunity against this human papilloma virus (HPV)-associated neoplasia has been signified. To stratify the possibility of HPV integration on HLA mutations, we measured the genotypic and phenotypic integrity of all available HLA class I loci in 30 cases of CC. Paired normal and cancer genomic DNA was analyzed with DNA typing trays, including 57 subtypes of HLA-A, 120 subtypes of HLA-B, and 60 subtypes of HLA-C. We demonstrated significant mutations of HLA genotype with reduced HLA molecule expression in CC. HPV coincide in >70% cases of aberrant HLA genes. Southern blot analysis revealed the presence of HPV DNA within the mutated HLA foci. Our study reveals a plausible role of HPV integration in the contexts of aberrant HLA genotypes in CC cells. Disruptions of the HLA genes can be possible tactics of HPV to attain the potential carcinogenetic purposes, and thus the cancer immune escape.

Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalClinical Immunology
Volume115
Issue number3
DOIs
Publication statusPublished - Jun 1 2005
Externally publishedYes

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Keywords

  • Cancer
  • DNA
  • Genotype
  • HLA
  • Human papilloma virus
  • Mutation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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