Intérêt d'une combinaison gélatine-agarose cryodesséchée pour l'extraction sélective de la fibronectine à partir du plasma humain

T. Burnouf, M. Allary, J. Guilbaud, E. Boschetti, J. Saint-Blancard

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We report here a simple procedure for the isolation of human plasma fibronectin by affinity adsorption on a new lyophylized adsorbent which is obtained by mixing and polymerizing agarose with gelatin. This method permits to obtain fibronectin and a fibronectin-depleted plasma without dilution. The yield of fibronectin is about 20 to 25 per cent. Fibronectin was identified by cross-reactions against specific antibodies. Its purity (≥99 per cent) was controled by immunoelectrophoresis and SDS-PAGE. Furthermore, its biological activity was demonstrated by a spreading test performed on BHK cells. Such a test can be used to determine the specific activity of extracted fibronectin considering that the number of spreaded cells placed in presence of exogenous fibronectin (5 μg/ml of culture medium) was 4.5 to 5 fold-superior to the control, after 30 minutes of incubation at 37°C.

Original languageFrench
Pages (from-to)559-570
Number of pages12
JournalRevue Francaise de Transfusion et Immuno-Hematologie
Volume24
Issue number6
DOIs
Publication statusPublished - 1981
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)
  • Hematology

Cite this

Intérêt d'une combinaison gélatine-agarose cryodesséchée pour l'extraction sélective de la fibronectine à partir du plasma humain. / Burnouf, T.; Allary, M.; Guilbaud, J.; Boschetti, E.; Saint-Blancard, J.

In: Revue Francaise de Transfusion et Immuno-Hematologie, Vol. 24, No. 6, 1981, p. 559-570.

Research output: Contribution to journalArticle

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AB - We report here a simple procedure for the isolation of human plasma fibronectin by affinity adsorption on a new lyophylized adsorbent which is obtained by mixing and polymerizing agarose with gelatin. This method permits to obtain fibronectin and a fibronectin-depleted plasma without dilution. The yield of fibronectin is about 20 to 25 per cent. Fibronectin was identified by cross-reactions against specific antibodies. Its purity (≥99 per cent) was controled by immunoelectrophoresis and SDS-PAGE. Furthermore, its biological activity was demonstrated by a spreading test performed on BHK cells. Such a test can be used to determine the specific activity of extracted fibronectin considering that the number of spreaded cells placed in presence of exogenous fibronectin (5 μg/ml of culture medium) was 4.5 to 5 fold-superior to the control, after 30 minutes of incubation at 37°C.

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