Insulin resistance induced by glucosamine in fructose-fed rats.

C. S. Chien, S. C. Cheng, H. T. Wu, C. W. Tsao, J. T. Cheng

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Glucosamine has been widely used to treat osteoporosis in clinic and it shows an effect on hexosamine biosynthetic pathway in glucose-induced insulin resistance. However, glucosamine and chronic hyperglycemia is not correlative. Thus, we used C (2)C (12) cell line to carry out the uptake assay of 2-[14C]-deoxy-d-glucose and [3H]-glucosamine. Glucosamine inhibited the in vitro glucose uptake in a concentration-dependent manner. The glucose transporter GLUT4 prepared for [3H]-glucosamine uptake showed a concentration-dependent competition between glucose and glucosamine uptake. The effects of glucosamine on glucose tolerance and homeostasis model assessment (HOMA) were also determined in normal Wistar and fructose-fed rats. Both plasma glucose levels and/or HOMA index were not changed in normal rats treated with glucosamine as compared with the saline-treated control. However, we found that glucosamine exhibited an effect on the expression of farnesoid X receptor in liver to exacerbate the values of HOMA and accelerate the development of insulin resistance in fructose-fed rats. Thus, glucosamine should be applied with caution in type-2 diabetic patients.

Original languageEnglish
Pages (from-to)542-547
Number of pages6
JournalHormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme
Volume41
Issue number7
DOIs
Publication statusPublished - Jul 1 2009
Externally publishedYes

Fingerprint

Glucosamine
Fructose
Insulin Resistance
Rats
Insulin
Glucose
Homeostasis
Hexosamines
Facilitative Glucose Transport Proteins
Biosynthetic Pathways
Hyperglycemia
Liver
Osteoporosis
Assays
Cells
Plasmas
Cell Line

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Insulin resistance induced by glucosamine in fructose-fed rats. / Chien, C. S.; Cheng, S. C.; Wu, H. T.; Tsao, C. W.; Cheng, J. T.

In: Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme, Vol. 41, No. 7, 01.07.2009, p. 542-547.

Research output: Contribution to journalArticle

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