Insulin-like growth factor II mRNA-binding protein 3 expression promotes tumor formation and invasion and predicts poor prognosis in oral squamous cell carcinoma

Chiao Ying Lin, Szu Ta Chen, Yung Ming Jeng, Cheng Chang Yeh, Han Yi Chou, Yi Ting Deng, Cheng Chi Chang, Mark Yen Ping Kuo

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Insulin-like growth factor II mRNA-binding protein 3 (IGF2BP3), an oncofetal RNA-binding protein, has been implicated in the enhancement of proliferation and invasion in various cancers. This study aimed to investigate the clinical significance and functional role of IGF2BP3 expression in oral squamous cell carcinoma (OSCC). Methods: IGF2BP3 expression in 93 OSCC patients was investigated using immunohistochemical staining and correlated with clinical parameters and patients' survival. The effect of IGF2BP3 on cell invasion ability was evaluated by RNA interference in OSCC cell line. Results: High expression of IGF2BP3 in OSCC was significantly correlated with large tumor size and lymph node metastasis. Kaplan-Meier analysis revealed that oral cancer patients with high IGF2BP3 expression had a significantly lower 5-year survival (P=0.0017). Multivariate analysis of clinical samples demonstrated IGF2BP3 to be an independent prognosis factor (P=0.003). Moreover, the IGF2BP3 shRNA significantly suppressed the invasion ability of OSCC in vitro, and the knockdown of endogenous IGF2BP3 expression also inhibited tumor formation in vivo. Conclusions: IGF2BP3 enhances cell invasion ability and tumorigenicity in human OSCC in vitro and in vivo. IGF2BP3 is an independent prognostic factor in patients with OSCC. Targeting of IGF2BP3 could potentially suppress the tumor growth and metastasis to improve the outcome of patients with OSCC.

Original languageEnglish
Pages (from-to)699-705
Number of pages7
JournalJournal of Oral Pathology and Medicine
Volume40
Issue number9
DOIs
Publication statusPublished - Oct 2011

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Insulin-Like Growth Factor II
Squamous Cell Carcinoma
Carrier Proteins
Messenger RNA
Neoplasms
Neoplasm Metastasis
RNA-Binding Proteins
Survival
Mouth Neoplasms
Kaplan-Meier Estimate
RNA Interference
Small Interfering RNA
Multivariate Analysis
Lymph Nodes
Staining and Labeling
Cell Line
Growth

Keywords

  • Invasion
  • Oral squamous cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Otorhinolaryngology
  • Oral Surgery
  • Periodontics

Cite this

Insulin-like growth factor II mRNA-binding protein 3 expression promotes tumor formation and invasion and predicts poor prognosis in oral squamous cell carcinoma. / Lin, Chiao Ying; Chen, Szu Ta; Jeng, Yung Ming; Yeh, Cheng Chang; Chou, Han Yi; Deng, Yi Ting; Chang, Cheng Chi; Kuo, Mark Yen Ping.

In: Journal of Oral Pathology and Medicine, Vol. 40, No. 9, 10.2011, p. 699-705.

Research output: Contribution to journalArticle

Lin, Chiao Ying ; Chen, Szu Ta ; Jeng, Yung Ming ; Yeh, Cheng Chang ; Chou, Han Yi ; Deng, Yi Ting ; Chang, Cheng Chi ; Kuo, Mark Yen Ping. / Insulin-like growth factor II mRNA-binding protein 3 expression promotes tumor formation and invasion and predicts poor prognosis in oral squamous cell carcinoma. In: Journal of Oral Pathology and Medicine. 2011 ; Vol. 40, No. 9. pp. 699-705.
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