Abstract
Treatment of quiescent Balb c 3T3 cells with insulin-like growth factor I (IGF I) resulted in the stimulation of proto-oncogene c-jun transcription. Cells exposed to cycloheximide and IGF I together showed super-induction of c-jun transcripts. Nuclear run-off assay revealed that IGF I up-regulated c-jun only while cycloheximide was present. The stability of c-jun mRNA was markedly increased in the cells treated with IGF I. These results suggest that IGF I controls the expression of c-jun by increasing the transcriptional activity and stabilizing the existing transcripts.
| Original language | English |
|---|---|
| Pages (from-to) | 524-531 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 183 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 16 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Insulin-like growth factor I stimulates transcription of the c-jun proto-oncogene in Balb c 3T3 cells. / Chiou, Shean Tai; Chang, Wen Chang.
In: Biochemical and Biophysical Research Communications, Vol. 183, No. 2, 16.03.1992, p. 524-531.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Insulin-like growth factor I stimulates transcription of the c-jun proto-oncogene in Balb c 3T3 cells
AU - Chiou, Shean Tai
AU - Chang, Wen Chang
PY - 1992/3/16
Y1 - 1992/3/16
N2 - Treatment of quiescent Balb c 3T3 cells with insulin-like growth factor I (IGF I) resulted in the stimulation of proto-oncogene c-jun transcription. Cells exposed to cycloheximide and IGF I together showed super-induction of c-jun transcripts. Nuclear run-off assay revealed that IGF I up-regulated c-jun only while cycloheximide was present. The stability of c-jun mRNA was markedly increased in the cells treated with IGF I. These results suggest that IGF I controls the expression of c-jun by increasing the transcriptional activity and stabilizing the existing transcripts.
AB - Treatment of quiescent Balb c 3T3 cells with insulin-like growth factor I (IGF I) resulted in the stimulation of proto-oncogene c-jun transcription. Cells exposed to cycloheximide and IGF I together showed super-induction of c-jun transcripts. Nuclear run-off assay revealed that IGF I up-regulated c-jun only while cycloheximide was present. The stability of c-jun mRNA was markedly increased in the cells treated with IGF I. These results suggest that IGF I controls the expression of c-jun by increasing the transcriptional activity and stabilizing the existing transcripts.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026579876&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(92)90513-K
DO - 10.1016/0006-291X(92)90513-K
M3 - Article
VL - 183
SP - 524
EP - 531
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -