Institutional spread of clonally related Serratia marcescens isolates with a novel AmpC cephalosporinase (S4): a 4-year experience in Taiwan

Wen Liang Yu, Wen Chien Ko, Kuo-Chen Cheng, Hui En Chen, Ching Chien Lee, Yin Ching Chuang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Resistance of Serratia marcescens, a nosocomial pathogen of Enterobacteriaceae, to the extended-spectrum β-lactams is usually mediated by an overproduced AmpC cephalosporinase. We aimed to characterize the molecular epidemiology and AmpC of S. marcescens isolates recovered from 1 medical center in southern Taiwan. AmpC-encoding genes for SRT families were investigated by polymerase chain reaction and DNA sequencing. From August 1999 through July 2003, 69 nonrepetitive bloodstream isolates were enrolled. Excluding 11 isolates, which also produced an extended-spectrum β-lactamase, 58 isolates carried an AmpC-encoding gene, including a novel S4 gene with 98% identity to SRT-1 gene (n = 50), SRT-2 gene (n = 3), SST-1 gene (n = 1), and others (n = 4). Isolates with S4 exhibited a phenotype of resistance to cefotaxime (CTX) but not ceftazidime. Genotype analysis by pulsed-field gel electrophoresis revealed that 45 (90%) of the isolates carrying S4 gene belonged to 2 major epidemic clones, including types A (n = 28) and B (n = 17). In conclusion, the AmpC-like S4 β-lactamase may confer CTX resistance of the S. marcescens population. Strains carrying the S4 gene with prolonged dissemination were closely related.

Original languageEnglish
Pages (from-to)460-467
Number of pages8
JournalDiagnostic Microbiology and Infectious Disease
Volume61
Issue number4
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Cephalosporinase
Serratia marcescens
Taiwan
Genes
Cefotaxime
Lactams
Ceftazidime
Molecular Epidemiology
Pulsed Field Gel Electrophoresis
Enterobacteriaceae
DNA Sequence Analysis
Clone Cells
Genotype
Phenotype
Polymerase Chain Reaction

Keywords

  • AmpC
  • Epidemiology
  • Serratia marcescens
  • SRT family

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

Cite this

Institutional spread of clonally related Serratia marcescens isolates with a novel AmpC cephalosporinase (S4) : a 4-year experience in Taiwan. / Yu, Wen Liang; Ko, Wen Chien; Cheng, Kuo-Chen; Chen, Hui En; Lee, Ching Chien; Chuang, Yin Ching.

In: Diagnostic Microbiology and Infectious Disease, Vol. 61, No. 4, 08.2008, p. 460-467.

Research output: Contribution to journalArticle

Yu, Wen Liang ; Ko, Wen Chien ; Cheng, Kuo-Chen ; Chen, Hui En ; Lee, Ching Chien ; Chuang, Yin Ching. / Institutional spread of clonally related Serratia marcescens isolates with a novel AmpC cephalosporinase (S4) : a 4-year experience in Taiwan. In: Diagnostic Microbiology and Infectious Disease. 2008 ; Vol. 61, No. 4. pp. 460-467.
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abstract = "Resistance of Serratia marcescens, a nosocomial pathogen of Enterobacteriaceae, to the extended-spectrum β-lactams is usually mediated by an overproduced AmpC cephalosporinase. We aimed to characterize the molecular epidemiology and AmpC of S. marcescens isolates recovered from 1 medical center in southern Taiwan. AmpC-encoding genes for SRT families were investigated by polymerase chain reaction and DNA sequencing. From August 1999 through July 2003, 69 nonrepetitive bloodstream isolates were enrolled. Excluding 11 isolates, which also produced an extended-spectrum β-lactamase, 58 isolates carried an AmpC-encoding gene, including a novel S4 gene with 98{\%} identity to SRT-1 gene (n = 50), SRT-2 gene (n = 3), SST-1 gene (n = 1), and others (n = 4). Isolates with S4 exhibited a phenotype of resistance to cefotaxime (CTX) but not ceftazidime. Genotype analysis by pulsed-field gel electrophoresis revealed that 45 (90{\%}) of the isolates carrying S4 gene belonged to 2 major epidemic clones, including types A (n = 28) and B (n = 17). In conclusion, the AmpC-like S4 β-lactamase may confer CTX resistance of the S. marcescens population. Strains carrying the S4 gene with prolonged dissemination were closely related.",
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